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14-3-3蛋白抗体说明书
  • 品牌:上海莼试
  • 产地:进口、国产
  • 货号:CS12324
  • 发布日期: 2019-02-25
  • 更新日期: 2024-03-28
产品详请
产地 进口、国产
品牌 上海莼试
保存条件 Store at -20 °C
货号 CS12324
应用范围 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
抗体名 Anti-pan 14-3-3
克隆性
靶点 详见说明书
适应物种 详见说明书
形态 详见说明书
宿主 详见说明书
亚型 IgG
标识物 详见说明书
浓度 1mg/1ml%
免疫原 KLH conjugated synthetic peptide derived from human pan 14-3-3

中文名称  14-3-3蛋白抗体说明书 

英文名称  Anti-pan 14-3-3

     14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta; KCIP 1; Protein 1054; Protein kinase C inhibitor protein 1; 14 3 3 protein delta; 14 3 3 protein gamma; YWHAB; YWHAZ; 14 3 3 protein Epsilon.

产品属性:

14-3-3蛋白抗体说明书       1mg/1ml

  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Pig, Cow, Sheep

产品类型   一抗  

研究领域     细胞生物 信号转导 细胞凋亡

蛋白分子量  predicted molecular weight: 28kDa 

       Lyophilized or Liquid

  KLH conjugated synthetic peptide derived from human pan 14-3-3

      IgG

纯化方法   affinity purified by Protein A

   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

14-3-3蛋白抗体说明书 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

(石蜡切片需做抗原修复) 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 14-3-3 are activates tyrosine and tryptophan hydroxylases in the presence of Ca (2+)/calmodulin-dependent protein kinase II, and strongly activates protein kinase C. Is probably a multifunctional regulator of the cell signaling processes mediated by both kinases. Activates the ADP-ribosyltransferase (exoS) activity of bacterial origin. 14-3-3 proteins are localized in neurons, and are axonally transported to the nerve terminals. They may be also present, at lower levels, in various other eukaryotic tissues. It belongs to the 14-3-3 family.

Function : Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions.

Subunit : Homodimer. Heterodimerizes with YWHAZ. Interacts with NDEL1, RGNEF and TIAM2 (By similarity). Interacts with HCV core protein. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B. Interacts with GAB2. Interacts with phosphorylated GRB10. Interacts with PKA-phosphorylated AANAT. Interacts with the phosphorylated (by AKT1) form of SRPK2.

Subcellular Location : Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Similarity : Belongs to the 14-3-3 family.

14-3-3蛋白是一个涉及调节细胞凋亡、促细胞分裂信号传导和细胞周期关卡的蛋白质家族。它被认为是通过与丝氨酸残基磷酸化的蛋白质的结合介导的信号传导中的关键调节物。通过与Bad(相关死亡因子)的结合, 14-3-3 蛋白由于将Bad隔离于胞液而防止了细胞凋亡。蛋白是14-3-3家族成员。它广泛分布于哺乳动物、两栖类、昆虫、植物和酵母菌的真核生物高度保守性多功能蛋白质。 目前已知至少有16个成员。此抗体识别分子量为30-31kDa的14-3-3蛋白αβγδε亚型。

实验流程:

全、新、优、品、好四大特点:

14-3-3蛋白抗体说明书 全:公司提供上万种产品,涵盖了生物试剂,elisa试剂盒,标准品,培养基,原装耗材,抗体、培养基、ATCC细胞等,基本上各种科研所需产品在我司都能找到。

新:产品更新速度较快,基本上每周都有新产品出现。

优:产品质量好,投诉比较少。

好:我公司具有优质的技术团队,产品一旦售出,实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

技术外包服务:

14-3-3蛋白抗体说明书 分子生物学:质粒抽提、PCRQ-PCRRT-PCR、分子生物学:基因合成、引物合成、基因测序、载体构建等

蛋白工程:原核、哺乳动物蛋白表达系统等

病毒包装:腺病毒、慢病毒等

抗体工程:磁珠分选、病理染色、WBELISAIPIFIHCFACSConfocal等等

细胞工程:细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

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