中心粒蛋白Nudel抗体规格 英文名称  Anti-NUDEL/NDEL1

中文名称  中心粒蛋白Nudel抗体规格 

别    名 A. nidulans; DKFZp451M0318; ENDOOLIGOPEPTIDASE A; EOPA; MITAP 1; MITAP1; Mitosin associated protein 1; Mitosin associated protein MITAP1; Mitosin-associated protein 1; Ndel 1; Ndel1; NDEL1_HUMAN; Nuclear distribution gene E like homolog 1; Nuclear distribution protein nudE like 1; Nuclear distribution protein nudE-like 1; NUDE like protein; NudE nuclear distribution gene E homolog like 1 A. nidulans; NudE nuclear distribution gene E homolog like 1; NUDEL; Protein Nudel.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit

产品类型   一抗  

研究领域    细胞生物 免疫学 染色质和核信号 神经生物学 信号转导 细胞周期蛋白

蛋白分子量  predicted molecular weight: 38kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human NUDEL (252-293aa)

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

中心粒蛋白Nudel抗体规格 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Nudel is important for normal cortical development. It is invovled in microtubule organization, nuclear translocation, and neuronal positioning in concert with various other factors (including Lis1, Pafah1b1, Pahfah1b2, dynein, dynorphin A and cdk5). Western blot analysis of mouse tissues shows abundant expression of Nudel in brain and testis, and much lower expression in heart, liver, kidney, and skeletal muscle. In fractionated rat brain, Nudel and Lis1 are both found in fractions enriched for postsynaptic density proteins. Immunostaining of embryonic day 18 mouse brain sections revealed staining of migrating neurons and thalamocortical axons of the intermediate zone of the developing cerebral cortex, as well as several other developing brain regions. The deduced protein contains 345 amino acids and has a calculated molecular mass of 38.4 kDa. It has a coiled coil motif (residues 19 to 201), followed by several potential phosphorylation sites for casein kinase II, protein kinase C or CDK5. Nudel shares about 50% identity with mouse and human NUDE proteins.

Function : Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.Subunit : Interacts with YWHAE. Interacts directly with NEFL and indirectly with NEFH. Interacts with microtubules (By similarity). Self-associates. Interacts with DISC1, dynein, dynactin, tubulin gamma, KATNA1, KATNB1, PAFAH1B1, PCM1 and PCNT. Interacts (via C-terminus) with CENPF. Interacts with ZNF365.Subcellular Location : Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.Tissue Specificity : Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.Post-translational modifications : Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.Palmitoylation at Cys-273 reduces affinity for dynein.Similarity : Belongs to the nudE family.Database links : UniProtKB/Swiss-Prot: Q9GZM8.1中心粒蛋白Nudel是动物细胞中，中心体微管组织的主要细胞器，是细胞质动力蛋白。

实验流程：

全、新、优、品、好四大特点：

中心粒蛋白Nudel抗体规格 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

正在热销的相关产品：

AFP ELISA Kit 大鼠甲种胎儿球蛋白/甲胎蛋白Multi-class antibodies规格： 48T

单抗 单抗Multi-class antibodies规格：

Rhesus antibody Rh FRMD1 FRMD1蛋白抗体 规格 0.2ml

AGEs ELISA Kit 大鼠晚期糖基化终末产物 96T

Neurofascin 英文名称： 神经束蛋白抗体 0.1ml

AHNAK 英文名称： 神经细胞分化相关蛋白AHNAK抗体 0.1ml

单抗 单抗Multi-class antibodies规格：

phospho-FAK(pTyr577) 磷酸化粘着斑激酶抗原Multi-class antibodies规格： 0.5mg

桥连整合因子蛋白抗体 Anti-BIN1 0.1ml

Rhesus antibody Rh Phospho-EEF2 (Thr56) 磷酸化真核翻译延长因子2抗体 规格 0.1ml

品名 规格 备注

ZNF342 英文名称： 锌指蛋白342抗体 0.2ml

phospho-DAP Kinase 1 (Ser 308) 英文名称： 磷酸化凋亡相关蛋白激酶1抗体 0.1ml

桥连整合因子蛋白抗体 Anti-BIN1 0.1ml

ZBTB34 英文名称： 锌指蛋白ZBTB34抗体 0.2ml

Doppel 英文名称： 朊蛋白DPL抗体 0.2ml

抗体 Anti-Anthrax 0.1ml

Anti-Torc2/Crtc2/FITC 荧光素标记CREB转录共激活因子TORC2抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-ERK1(Thr202/Tyr204) 磷酸化原活化蛋白激酶1抗体 规格 0.1ml

Defensin Beta1(human) 防御素β1抗原(人)Multi-class antibodies规格： 0.5mg

EB增菌液250g用于单增李氏菌选择性增菌培养(SN标准)

DNA酶甲基绿琼脂基础 Dnase Agar Base with Methyl Green 用于弯曲杆菌的DNA酶水解试验(GB标准)

BL培养基 Bile Lactose Broth 250克 大肠杆菌和绿脓杆菌的增菌培养

22soVitaleX添加剂0支/盒每支用于Thayer-Martin选择性琼脂基础用于制备Thayer-Martin选择性琼脂或MTMⅡ琼脂incubationmedia22soVitaleX添加剂0支/盒每支用于Thayer-Martin选择性琼脂基础用于制备Thayer-Martin选择性琼脂或MTMⅡ琼脂

结晶紫中性红胆盐葡萄糖琼脂(VRBGA) 250g 用于奶粉中阪崎杆菌的分离培养(FDA、SN方法)

HB0235-7

卵磷脂吐温80营养琼脂 1000(g) incubation media 卵磷脂吐温80营养琼脂 1000(g)

大肠菌群大肠杆菌显色培养基(ECC) 1000ml 用于大肠菌群和大肠杆菌的快速检测和计数

改良rv培养基250incubationmedia改良rv培养基250

ReinforcedClostridiumAgar

中心粒蛋白Nudel抗体规格 绿脓菌素测定用培养基（PDP）  规格：  250g  用途：  用于鉴别绿脓杆菌产生绿脓菌素试验。(《中华人民共和国药典》2010年版)

胆盐乳糖培养基（BL）  规格：  250g  用途：  用于药品中大肠杆菌和绿脓杆菌的增菌培养。（《中华人民共和国药典》2010年版）

冻干血浆  规格：  0.5ml/支X10  用途：  用于金黄色葡萄球菌血浆凝固酶试验。

卵黄琼脂培养基基础  规格：  250g  用途：  用于金黄色葡萄球菌的选择性分离培养和计数(《中华人民共和国药典》2010年版)。

技术外包服务:

★中心粒蛋白Nudel抗体规格 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。