中文名称  磷酸化中心粒蛋白Nudel抗体费用 

英文名称  Anti-phospho-NDEL1(Ser242)

别    名 NDEL1(phospho S242); Nudel(p-Ser242); A. nidulans; DKFZp451M0318; ENDOOLIGOPEPTIDASE A; EOPA; MITAP 1; MITAP1; Mitosin associated protein 1; Mitosin associated protein MITAP1; Mitosin-associated protein 1; Ndel 1; Ndel1; NDEL1_HUMAN; Nuclear distribution gene E like homolog 1; Nuclear distribution protein nudE like 1; Nuclear distribution protein nudE-like 1; NUDE like protein; NudE nuclear distribution gene E homolog like 1 A. nidulans; NudE nuclear distribution gene E homolog like 1; NUDEL; Protein Nudel.

产品属性：

磷酸化中心粒蛋白Nudel抗体费用 浓    度  1mg/1ml

规 格  0.1ml/100μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit

产品类型   一抗 磷酸化抗体  

研究领域    细胞生物 免疫学 神经生物学 信号转导 细胞周期蛋白 细胞分化

蛋白分子量  predicted molecular weight: 38kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human NDEL1 around the phosphorylation site of Ser242

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化中心粒蛋白Nudel抗体费用 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Nudel is important for normal cortical development. It is invovled in microtubule organization, nuclear translocation, and neuronal positioning in concert with various other factors (including Lis1, Pafah1b1, Pahfah1b2, dynein, dynorphin A and cdk5). Western blot analysis of mouse tissues shows abundant expression of Nudel in brain and testis, and much lower expression in heart, liver, kidney, and skeletal muscle. In fractionated rat brain, Nudel and Lis1 are both found in fractions enriched for postsynaptic density proteins. Immunostaining of embryonic day 18 mouse brain sections revealed staining of migrating neurons and thalamocortical axons of the intermediate zone of the developing cerebral cortex, as well as several other developing brain regions. The deduced protein contains 345 amino acids and has a calculated molecular mass of 38.4 kDa. It has a coiled coil motif (residues 19 to 201), followed by several potential phosphorylation sites for casein kinase II, protein kinase C or CDK5. Nudel shares about 50% identity with mouse and human NUDE proteins.

Function : Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.Subunit : Interacts with YWHAE. Interacts directly with NEFL and indirectly with NEFH. Interacts with microtubules (By similarity). Self-associates. Interacts with DISC1, dynein, dynactin, tubulin gamma, KATNA1, KATNB1, PAFAH1B1, PCM1 and PCNT. Interacts (via C-terminus) with CENPF. Interacts with ZNF365.Subcellular Location : Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.Tissue Specificity : Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.Post-translational modifications : Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.Palmitoylation at Cys-273 reduces affinity for dynein.Similarity : Belongs to the nudE family.Database links : UniProtKB/Swiss-Prot: Q9GZM8.1中心粒蛋白Nudel是动物细胞中，中心体微管组织的主要细胞器，是细胞质动力蛋白。

实验流程：

全、新、优、品、好四大特点：

磷酸化中心粒蛋白Nudel抗体费用 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

技术外包服务:

★磷酸化中心粒蛋白Nudel抗体费用 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

正在热销的相关产品：

Anti-Plexin B1/Semaphorin receptor/FITC 荧光素标记神经丛蛋白B1抗体IgGMulti-class antibodies规格： 0.2ml

标准胎血清Multi-class antibodies规格： 200ml

人类状瘤病毒抗体 Anti-HPV 0.1ml

Goat anti-Rabbit IgG whole serum 羊抗兔IgG抗血清 1ml

Fibronectin 英文名称： 纤维连接蛋白抗体 0.1ml

Rhesus antibody Rh Phospho-RCC1 (Ser11) 磷酸化染色体浓缩调控蛋白1抗体 规格 0.1ml

标准胎血清Multi-class antibodies规格： 200ml

GAD-67 (glutamate decarboxylase 67) 谷氨酸脱羧酶-67抗原Multi-class antibodies规格： 0.5mg

CLEC7A Dectin-1 / CLEC7A 兔单抗 (PE) Mouse

Rhesus antibody Rh phospho-DDX58 (Ser8) 磷酸化DDX58抗体 规格 0.1ml

纤维素膜(15×60cm 0.45um) 1张 Pall-Gelman

ZNF312 英文名称： 锌指蛋白312抗体 0.1ml

DCIR/CLEC4A 英文名称： C型凝集素结构域家族4成员A抗体 0.2ml

CLEC7A Dectin-1 / CLEC7A 兔单抗 (PE) Mouse

ZBTB48 英文名称： 锌指蛋白855抗体 0.2ml

DACT1 英文名称： 新基因3蛋白抗体 0.2ml

膜粘连蛋白 V抗体 Anti-Annexin V 0.1ml

Anti-Zyxin/FITC 荧光素标记斑联蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-ERK1/MAPK-1/2(Thr183/185) 磷酸化原活化蛋白激酶1/2抗体 规格 0.1ml

DCX(Doublecortin) 双皮质素抗原Multi-class antibodies规格： 0.5mg

铁细菌培养基250g用于铁细菌的分离培养

DPBS，(10X)，(IVD) 含钙镁离子，不含酚红 14080-055 500ml incubation media DPBS，(10X)，(IVD) 含钙镁离子，不含酚红 14080-055 500ml

寄生曲霉 病原菌 支/瓶

PALCAMAgar

沉降菌测试培养基

多粘菌素B/支*5用水溶解后添加于HB0256中

卵黄琼脂基础 Egg Yolk Agar Base 加入卵黄，用于厌氧梭状芽孢杆菌的分离培养

葡萄糖胰酪胨琼脂 Dextrose Tryptone Agar 250克 嗜热菌芽孢(需氧芽孢总数、平酸芽孢和厌氧芽孢)分离培养

Korser氏枸椽酸盐肉汤于细菌枸椽酸盐试验(SN标准)incubationmediaKorser氏枸椽酸盐肉汤于细菌枸椽酸盐试验(SN标准)

PBSbroth

磷酸化中心粒蛋白Nudel抗体费用 四酸亮绿培养基基础（TTB）  规格：  250g  用途：  用于沙门氏菌选择性增菌培养（《中华人民共和国药典》2010年版）。

曙红亚甲蓝琼脂培养基（EMB）  规格：  250g  用途：  用于药品中大肠杆菌、沙门氏菌检测。（《中华人民共和国药典》2010年版）

三糖铁琼脂培养基（TSI）  规格：  250g  用途：  用于鉴别肠道菌发酵蔗糖、乳糖、葡萄糖及产生的生化反应（《中华人民共和国药典》2010年版...

营养肉汤培养基（普通肉汤培养基）  规格：  250g  用途：  用于一般细菌培养、复壮、增菌等，也可用于消毒剂定性消毒效果测定。（《中华人民共和国药典》20...