抗体的生物素化标记实验要点：

1. 核孔糖蛋白P62抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-Nucleoporin p62 

中文名称   核孔糖蛋白P62抗体说明书 

别    名  NUP62_HUMAN; 62 kDa nucleoporin; DKFZp547L134; FLJ20822; FLJ43869; MGC841; Nuclear pore glycoprotein p62; nucleoporin 62kDa; nucleoporin p62; nucleoporin p62KD; NUP62; NUP62 protein; Nucleoporin Nup62; p62.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit 

产品类型   一抗  

研究领域    细胞生物 免疫学 染色质和核信号 神经生物学 信号转导 细胞凋亡 生长因子和激素 细胞类型标志物 表观遗传学

蛋白分子量  predicted molecular weight: 53kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Nucleoporin p62 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

核孔糖蛋白P62抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq].

Function : Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex.Subunit : Component of the p62 complex, a complex at least composed of NUP62, NUP54, and NUPL1 (By similarity). Interacts with C11orf73/Hikeshi.Subcellular Location : Nucleus, nuclear pore complex. Cytoplasm, cytoskeleton, spindle pole. Note=Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle.Post-translational modifications : O-glycosylated. Contains about 10 N-acetylglucosamine side chain sites predicted for the entire protein, amongst which only one in the C-terminal.DISEASE : Infantile striatonigral degeneration (SNDI) [MIM:271930]: Neurological disorder characterized by symmetrical degeneration of the caudate nucleus, putamen, and occasionally the globus pallidus, with little involvement of the rest of the brain. The clinical features include developmental regression, choreoathetosis, dystonia, spasticity, dysphagia, failure to thrive, nystagmus, optic atrophy, and mental retardation. Note=The disease is caused by mutations affecting the gene represented in this entry.Similarity : Belongs to the nucleoporin NSP1/NUP62 family.Database links : UniProtKB/Swiss-Prot: P37198.3

抗体的鉴定：

1）核孔糖蛋白P62抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-RPS6/FITC 荧光素标记S6核糖体蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Tachyplesin I(Tachyplesin-1 precursor ) 抗菌肽Multi-class antibodies规格： 0.5mg

中脑核仁蛋白1抗体 Anti-Midnolin isoform Protein 1 0.1ml

S-100/S100A1 英文名称： S100A1抗体 0.1ml

Fetuin beta 英文名称： 胎球蛋白B/Fetuin β抗体 0.2ml

Rhesus antibody Rh Phospho-PEA15(Ser116) 磷酸化星形胶质细胞PEA15抗体 规格 0.1ml

Tachyplesin I(Tachyplesin-1 precursor ) 抗菌肽Multi-class antibodies规格： 0.5mg

GPA33(glycoprotein A33 transmembrane) 糖蛋白A33(跨膜)抗原Multi-class antibodies规格： 0.5mg

MMP1 MMP-1 鼠单抗 (PE) Human

Rhesus antibody Rh phospho-CTNNBIP1 (Thr43/Ser50) 磷酸化β链接素相互作用蛋白1抗体 规格 0.1ml

Western洗涤液 100ml 自产

ZBTB39 英文名称： 锌指蛋白ZBTB39抗体 0.2ml

DNA PKcs 英文名称： DNA依赖蛋白激酶催化亚基抗体 0.1ml

MMP1 MMP-1 鼠单抗 (PE) Human

5HT5B Receptor 英文名称： 5-羟色胺受体5B抗体 0.1ml

ENPP6 英文名称： 磷酸酶/磷酸二酯酶家族成员6抗体 0.2ml

三磷酸腺苷结合盒亚家族G1抗体 Anti-ABCG1 0.1ml

Anti-WWOX /FITC 荧光素标记包含氧化还原酶的WW域抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-FLT3 (Tyr969) 磷酸化FMS样酪氨酸激酶3 规格 0.1ml

Collagen IV (Anti-Collagen Type IV ) IV型胶原抗体(Collagen Ⅳ Ⅳ 型胶原)Multi-class antibodies规格： 0.5mg

卫矛醇半固体琼脂培养基，用于细菌卫予醇发酵试验(SN标准)

EthylVioletAziodeBroth

葡萄球菌增菌肉汤 Staphylococcus Enrichment Broth 250 用于凝固酶阳性葡萄球菌的选择性增菌

Nitsch&Nitsch培养基250g/瓶用于植物组织培养incubationmediaNitsch&Nitsch培养基250g/瓶用于植物组织培养

含225ml7.5%氯肉汤均质袋 10个/包 用于金葡菌前增菌培养

SLBBroth

亮绿胆盐肉汤 标准方法培养基用于水和食品中大肠菌群的确认和完全测试 500g incubation media 亮绿胆盐肉汤 标准方法培养基用于水和食品中大肠菌群的确认和完全测试 500g

斑褶菇 支/瓶

BufferedPeptoneWater

Nitratepeptonewatermedium

核孔糖蛋白P62抗体说明书 10%NaCl胨水生化鉴定管  规格：  20支/盒  用途：  用于弧菌的耐盐试验。

9%NaCl胨水生化鉴定管  规格：  20支/盒  用途：  用于弧菌的耐盐试验。

8%NaCl胨水生化鉴定管  规格：  20支/盒  用途：  用于弧菌的耐盐试验。

7%NaCl胨水生化鉴定管  规格：  20支/盒  用途：  用于弧菌的耐盐试验。