产品订购信息：
英文名称  Anti-NEK2 

中文名称   中心体相关蛋白激酶Nek2抗体费用 

别    名  Serine/threonine-protein kinase Nek2; HSPK 21; Never in mitosis A-related kinase 2; NimA-related protein kinase 2; NimA-like protein kinase 1;NEK2_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit 

产品类型   一抗  

研究领域     细胞生物 免疫学 细胞周期蛋白 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 52kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human NEK2 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

中心体相关蛋白激酶Nek2抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.

Function : Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.Subunit : Isoform 1, isoform 2 and isoform 4 form homo- and heterodimers. Interacts with NECAB3 and HMGA2 (By similarity). Isoform 1 interacts with CDC20, CTNB1, MAD1L1, MAPK, NEK11, NPM1, NDC80, PCNT and SGOL1. Isoform 1 interacts with STK3/MST2 (via SARAH domain) and SAV1 (via SARAH domain). Isoform 1 and isoform 2 interact with MAD2L1. Isoform 1 and isoform 4 interact with PPP1CA and PPP1CC.Subcellular Location : Isoform 1: Nucleus. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere (By similarity). Note=STK3/MST2 and SAV1 are required for its targeting to the centrosome. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end.Isoform 2: Cytoplasm. Note=Predominantly cytoplasmic. <> Isoform 4: Nucleus. Cytoplasm, cytoskeleton, centrosome. Note=Predominantly brnuclear.Tissue Specificity : Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors.Post-translational modifications : Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome.Similarity : Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.Contains 1 protein kinase domain.Database links : UniProtKB/Swiss-Prot: P51955.1

H1N1(Influenza A virus ,hemagglutinin) 病毒A抗原Multi-class antibodies规格： 0.5mg

Sox2 Sox2 鼠单抗 (FITC) Human

Rhesus antibody Rh phospho-c-Raf(Ser471) 磷酸化原癌基因c-Raf抗体 规格 0.1ml

细胞核蛋白与细胞浆蛋白抽提试剂 100次

Phospho-ZAP70 (Tyr315 + Tyr319) 英文名称： 磷酸化zeta相关蛋白70抗体 0.1ml

phospho-DDX5 (Tyr593) 英文名称： 磷酸化三磷酸腺苷依赖解旋酶ddx5抗体 0.1ml

Sox2 Sox2 鼠单抗 (FITC) Human

phospho-GSK-3 Beta(pSer9) 磷酸化葡萄糖合成激酶-3β抗原Multi-class antibodies规格： 0.5mg

S100A9 S100A9 鼠单抗 (PE) Human

Rhesus antibody Rh phospho-CREM (Ser271 +Ser 274) 磷酸化环磷酸腺苷反应元件调节蛋白抗体 规格 0.1ml

人淋巴细胞分离液(Lymphocyte Separation Medium) 250ml 国产

ZBED3 英文名称： 锌指蛋白BED3抗体 0.2ml

Desmocollin 2 英文名称： 桥粒糖蛋白2/桥粒糖蛋白3抗体 0.1ml

S100A9 S100A9 鼠单抗 (PE) Human

SIPA1L2 英文名称： 信号诱导增殖相关蛋白1样蛋白2抗体 0.2ml

EPLIN 英文名称： 恶性丢失蛋白EPLIN抗体 0.2ml

8-羟基脱氧鸟苷抗体 Anti-8-OHdG 0.1ml

Anti-WDR26/FITC 荧光素标记Gβ类似蛋白WDR26抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-FOXO4 (Ser197) 磷酸化叉头蛋白4抗体 规格 0.1ml

AU5 tag AU5 tag标签抗原Multi-class antibodies规格： 0.5mg

HelicobacterPyloriMedium

Fluid Selenite - Cystine Medium incubation media Fluid Selenite - Cystine Medium

巴西毛壳 支/瓶

碱性蛋白胨水225ml/袋*霍乱弧菌选择性增菌培养(SN标准)

7.5%SodiumChlorideBroth

PseudomonasCFCSelectiveAgaradditives

李斯特菌增菌肉汤 食品中李斯特菌的选择培养，用于非乳制品及加工后食品中单核细胞增生李斯特菌的选择性增加 500g incubation media 李斯特菌增菌肉汤 食品中李斯特菌的选择培养，用于非乳制品及加工后食品中单核细胞增生李斯特菌的选择性增加 500g

贝酵母 用于酿造白葡萄酒，耐酒精18%。 支/瓶

3%NaClAlkalinePeptoneWater

ThioglycollateMedium(withoutAgar)

中心体相关蛋白激酶Nek2抗体费用 1%NaCl蛋白胨水生化鉴定管  规格：  20支/盒  用途：  供耐的弧菌的靛基质试验用。

1%NaCl精酸双水解酶生化鉴定管  规格：  20支/盒  用途：  用于鉴别耐的弧菌精酸基酸脱羧基形成胺使培养基变碱的能力。（鉴定霍乱弧菌）

1%NaCl基酸脱羧酶对照生化鉴定管  规格：  20支/盒  用途：  用于鉴别耐的弧菌基酸基酸脱羧基形成胺使培养基变成碱的能力。（鉴定霍乱弧菌）

1%NaCl精酸脱羧酶生化鉴定管  规格：  20支/盒  用途：  用于鉴别耐的弧菌鸟酸基酸脱羧基形成胺使培养基变碱的能力。

抗体的生物素化标记实验要点：

1. 中心体相关蛋白激酶Nek2抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）中心体相关蛋白激酶Nek2抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。