抗体的生物素化标记实验要点：

1. 膜相关蛋白样蛋白NUMBL抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-NUMBL 

中文名称   膜相关蛋白样蛋白NUMBL抗体说明书 

别    名  CAG 3A; CAG3A; CTG 3a; CTG3a; NBL; NUMB Drosophilia Homolog Like; Numb homolog (Drosophila) like; Numb homolog like; Numb like protein; NUMB R; Numb-like protein; Numb-R; Numb-related protein; NUMBL; NUMBL_HUMAN; NUMBR; TNRC 23; TNRC23.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep 

产品类型   一抗  

研究领域    细胞生物 神经生物学 信号转导 细胞凋亡 转录调节因子 细胞分化 细胞膜蛋白

蛋白分子量  predicted molecular weight: 65kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human NUMBL (75-120aa) 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

膜相关蛋白样蛋白NUMBL抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 In Drosophila, neuronal cell fate decisions are directed by NUMB, a signaling adapter protein with two protein-protein interaction domains, namely a phosphotyrosine-binding domain and a proline-rich SH3-binding region (PRR). The mammalian NUMB homolog plays a role in the determination of cell fate during development and binds with a variety of proteins, including Eps15, LNX1 and Notch 1. NumbL (NUMB-like protein), also known as Numb-R, NBL, CAG3A, CTG3a, NUMBLIKE or TNRC23, is a 609 amino acid cytoplasmic protein that, like NUMB, is thought to play a role in cell fate. Expressed at high levels in developing brain tissue, NumbL contains one PID (phosphotyrosine interaction domain) and plays an important role in neuronal differentiation, possibly associating with Eps15 and Notch 1. In mice, deletion of the NumbL gene is associated with early embryonic death, suggesting an essential role for NumbL in early development.

Function : Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.Subunit : Interacts (via PTB domain) with MAP3K7IP2 (via C-terminal). Interacts (via C-terminal) with TRAF6 (via TRAF domains). Associates with EPS15 and NOTCH1.Subcellular Location : Cytoplasm. Symmetrically distributed throughout the cytoplasm in non dividing neuroblasts of the CNS.Similarity : Contains 1 PID domain.Database links : UniProtKB/Swiss-Prot: Q9Y6R0.1

抗体的鉴定：

1）膜相关蛋白样蛋白NUMBL抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-PS-1/FITC 荧光素标记早老素蛋白-1抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-mouse IgG1 kappa-chain Whole serum 兔抗小鼠k-链抗血清Multi-class antibodies规格： 1ml

白介素4抗体 Anti-IL-4 0.1ml

Rabbit anti-Guinea pig IgG whole serum 兔抗豚鼠IgG抗血清 1ml

Phospho-FGFR1 (Tyr154) 英文名称： 磷酸化碱性成纤维细胞生长因子受体1抗体 0.1ml

Rhesus antibody Rh Phospho-PSD93 (Tyr340) 磷酸化突触后密度蛋白93抗体 规格 0.1ml

Rabbit Anti-mouse IgG1 kappa-chain Whole serum 兔抗小鼠k-链抗血清Multi-class antibodies规格： 1ml

Muc-1/CD227 Anti-gen (mucin-1) 粘蛋白-1/上皮膜抗原Multi-class antibodies规格： 0.5mg

Anti-Anthrax 炭疽杆菌抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-Bax(Ser184) 磷酸化Bax抗体 规格 0.1ml

兔抗人 IgA/FITC 0.2ml 绿色荧光，Dako公司

VWCE 英文名称： VWCE抗体 0.2ml

DIRAS2 英文名称： DIRAS2蛋白抗体 0.2ml

Anti-Anthrax 炭疽杆菌抗体Multi-class antibodies规格： 0.2ml

Sec8 英文名称： 胞吐分泌蛋白Sec8抗体 0.2ml

EDRF1 英文名称： 红细胞分化相关因子1抗体 0.2ml

组织蛋白酶H抗体 Anti-cath-H/CH 0.1ml

Anti-UMOD/FITC 荧光素标记UMOD抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-Histone H3 (Thr11) 磷酸化组蛋白H3抗体 规格 0.1ml

CXCL10/IP-10 CXCL10趋化因子10/干扰素诱导蛋白10抗原Multi-class antibodies规格： 0.5mg

纤维二糖5每菌添加于

JMMediumBase

2216E琼脂 2216E　Agar 250 用于海生细菌的培养和计数

肠道菌增菌肉汤(EE肉汤)250g/瓶用于肠道菌的增菌培养incubationmedia肠道菌增菌肉汤(EE肉汤)250g/瓶用于肠道菌的增菌培养

品红亚琼脂平板(9cm) 10个/包 用于饮用水、水源水中总大肠菌群的选择性分离和确证

FluidTetrathionateMedium

金黄色葡萄球菌显色培养基 500 mL/瓶 incubation media 金黄色葡萄球菌显色培养基 500 mL/瓶

Fraser肉汤增菌液FB1配套试剂 2x5支 试剂A和试剂B各一支添加于225ml(026080)中配成FB1增菌液。

琼脂培养基MAgarmediumM(Triplesugar,ironagar)用于肠杆菌科细菌的生化反应筛选。

乳酸杆菌选择性琼脂 250g 用于乳酸杆菌检测和分离培养

膜相关蛋白样蛋白NUMBL抗体说明书 煌绿乳糖胆盐肉汤(BGLB) 250(g)  incubation media 煌绿乳糖胆盐肉汤(BGLB) 250(g)

煌绿乳糖胆盐肉汤(BGLB) 1000(g)  incubation media 煌绿乳糖胆盐肉汤(BGLB) 1000(g)

EC肉汤 250(g)  incubation media EC肉汤 250(g)

EC肉汤 1000(g)  incubation media EC肉汤 1000(g)