抗体的生物素化标记实验要点：

1. 磷酸化间变性激酶核相互作用伴侣蛋白抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-phospho-NIPA(Ser354) 

中文名称   磷酸化间变性激酶核相互作用伴侣蛋白抗体品牌 

别    名  NIPA (phospho S354); NIPA (phospho Ser354); p-NIPA (S354); p-NIPA (Ser354); hNIPA; Nuclear interacting partner of ALK; Nuclear interacting partner of anaplastic lymphoma kinase; ZC3HC1; Zinc finger C3HC type containing 1; NIPA_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Horse, Rabbit 

产品类型   一抗 磷酸化抗体  

研究领域     细胞生物 免疫学 染色质和核信号 信号转导 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 55kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthesised phosphopeptide derived from human NIPA around the phosphorylation site of Ser354 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

磷酸化间变性激酶核相互作用伴侣蛋白抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The regulated oscillation of protein expression is an essential mechanism of cell cycle control. The SCF class of E3 ubiquitin ligases is involved in this process by targeting cell cycle regulatory proteins for degradation by the proteasome, with the F-box subunit of the SCF specifically recruiting a given substrate to the SCF core. NIPA (nuclear interaction partner of ALK) is a human F-box-containing protein that defines an SCF-type E3 ligase (SCFNIPA) controlling mitotic entry. Assembly of this SCF complex is regulated by cell-cycle-dependent phosphorylation of NIPA, which restricts substrate ubiquitination activity to interphase. Nuclear cyclin B1 is a substrate of SCFNIPA. Inactivation of NIPA by RNAi results in nuclear accumulation of cyclin B1 in interphase, activation of cyclin B1-Cdk1 kinase activity, and premature mitotic entry. Thus, SCFNIPA-based ubiquitination may regulate S-phase completion and mitotic entry in the mammalian cell cycle.

Function : Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.Subunit : Interacts with the NPM-ALK fusion protein in a tyrosine phosphorylation-dependent manner. Interacts with SKP1. Component of a SCF(NIPA) E3 complex with SKP1, RBX1 and CUL1 when not phosphorylated on Ser-354. Interacts with CCNB1.Subcellular Location : Nuclear.Tissue Specificity : Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes.Post-translational modifications : hosphorylated. Phosphorylated on Ser residues at G2/M phase, but not during S and G0 phases. May also be weakly phosphorylated on Tyr residues. Ser-354 phosphorylation, a major site during the course of cell-cycle-dedendent phosphorylation, results in its dissociation from the SCF(NIPA) complex, thereby preventing CCNB1 degradation leading to mitotic entry.Similarity : Contains 1 C3HC-type zinc finger.Database links : UniProtKB/Swiss-Prot: Q86WB0.1

抗体的鉴定：

1）磷酸化间变性激酶核相互作用伴侣蛋白抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

IL-13(Interleukin-13) 白介素13抗原Multi-class antibodies规格： 0.5mg

Anti-phospho-Acinus(Ser1180) 磷酸化腺泡Acinus蛋白抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-CDKN1B/P27kip1 (Ser10) 磷酸化P27抗体/周期素依赖激酶抑制剂 规格 0.1ml

IgG 干粉 10mg/50mg/100mg 国产

Wnt16 英文名称： 信号通路Wnt16抗体 0.2ml

Delta 4 英文名称： Notch信号通路Delta样配体4抗体 0.1ml

Anti-phospho-Acinus(Ser1180) 磷酸化腺泡Acinus蛋白抗体Multi-class antibodies规格： 0.1ml

AMA(Mouse Anti-myocardial antibody) ELISA Kit 小鼠抗心肌抗体Multi-class antibodies规格： 48T

Anti-CD83/Cell surface protein HB15/FITC 荧光素标记CD83抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Doublecortin 双皮质素抗体 规格 0.1ml

CA50 上皮类癌相关抗原 96T

MGLUR2 + MGLUR3 英文名称： 促代谢型谷酸受体2+3抗体 0.2ml

ADCK2 英文名称： ADCK2蛋白抗体 0.2ml

Anti-CD83/Cell surface protein HB15/FITC 荧光素标记CD83抗体IgGMulti-class antibodies规格： 0.2ml

Anti-A/H5N1-M2 /Biotin 生物素标记A型病毒H5N1-M2蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Anti-PAX2/FITC 荧光素标记配对盒基因2抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh BCHE (NT) 丁酰胆碱酯酶抗体(N端) 规格 0.1ml

AQP-2 (aquaporin Protein-2) 水通道蛋白-2(抗原) 0.5mg

GABA A Receptor alpha 1 英文名称： G1基A型受体α1抗体 0.1ml

Rhesus antibody Rh Rabbit Anti-rat IgM/Cy7 Cy7标记的兔抗大鼠IgM 规格 0.1ml

Anti-PAX2/FITC 荧光素标记配对盒基因2抗体IgGMulti-class antibodies规格： 0.2ml

Bordet-GengouAgarMedium

DMEM(低糖)，液体 "含1000mg/l葡萄糖，L-谷氨酰胺， incubation media DMEM(低糖)，液体 "含1000mg/l葡萄糖，L-谷氨酰胺，

胶质芽孢杆菌 支/瓶

NA平板(加青霉素酶)(9cm)细菌计数、不含糖，可作血琼脂基础和传代用

XLDAgar

ThioglycollateMedium

马铃薯-葡萄糖琼脂培养基(不含氯霉素) 250(g) incubation media 马铃薯-葡萄糖琼脂培养基(不含氯霉素) 250(g)

察氏培养基 Czapek Dox Agar 250克 用于酵母菌的培养

多粘菌素B2.5万单位/支*5添加于HB4incubationmedia多粘菌素B2.5万单位/支*5添加于HB4131中

BufferedPeptoneWater

磷酸化间变性激酶核相互作用伴侣蛋白抗体品牌 亚铋琼脂(BS)  规格：  250g  用途：  用于沙门氏菌特别是伤寒沙门氏菌的选择性分离培养(GB 4789.4－2010，SN/T2206.1-2008和SN0170-92...

盐增菌液(SF)  规格：  100g  用途：  用于沙门氏菌选择性增菌培养。(GB18466-2005)

三糖铁琼脂（TSI）  规格：  250g  用途：  用于鉴别肠道菌发酵蔗糖、乳糖、葡萄糖及产生的生化反应（GB/T4789.36－2008，GB4789.4－2...

Pfizer 选择性肠球菌琼脂培养基  规格：  100g  用途：  用于多管发酵法测定水中肠球菌的确证试验(CJ/T148-2001和SN/T2206.5-2009)。