产品订购信息：
英文名称  Anti-H1N1 Nucleoprotein 

中文名称   A型核蛋白抗体规格 

别    名  Nucleoprotein; NP; Nucleocapsid protein; Protein N; Influenza A virus H1N1; H3N2 Nucleoprotein; H9N2 Nucleoprotein; H2N2 Nucleoprotein; H3N8 Nucleoprotein; H7N7 Nucleoprotein; H5N1 Nucleoprotein.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Influenza A virus 

产品类型   一抗  

研究领域    细胞生物 免疫学 细菌及

蛋白分子量  predicted molecular weight: 56kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from Influenza A virus H1N1 Nucleoprotein 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

A型核蛋白抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.

Function : Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.Subunit : Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases.Subcellular Location : Virion (Potential). Host nucleus.Similarity : Belongs to the influenza viruses nucleoprotein family.Database links : UniProtKB/Swiss-Prot: B4URE0.1

P53 大鼠P53蛋白Multi-class antibodies规格： 48T

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IgHV(Human immunoglobulin heavy chain variable region) ELISA Kit 人免疫球蛋白重链可变区 96T

SIGLEC10 英文名称： 唾液酸结合性免疫球蛋白样凝集素抗体 0.2ml

C21orf33 英文名称： 21号染色体开放阅读框33抗体 0.2ml

Anti-Phospho-EGFR(Tyr1173) 磷酸化表皮生长因子受体抗体Multi-class antibodies规格： 0.1ml

Anti-DcR2/CD264 /FITC 荧光素标记抗诱捕受体2抗体IgGMulti-class antibodies规格： 0.2ml

Anti-PDE4D/FITC 荧光素标记磷酸二酯酶4D抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Batroxobin 蛇毒巴曲酶抗体 规格 0.2ml

5-HTR3(5-hydroxytryptamine (serotonin) receptor 3a) 5-羟色胺受体3抗原 0.5mg

mGluR5 英文名称： 促代谢型谷酸受体5抗体 0.1ml

Rhesus antibody Rh Rabbit Anti-rat IgG/PE-Cy3 PE-Cy3标记的兔抗大鼠IgG 规格 0.1ml

Anti-PDE4D/FITC 荧光素标记磷酸二酯酶4D抗体IgGMulti-class antibodies规格： 0.2ml

Human hypothetical protein LOC433328 ELISA Kit 人假定蛋白LOC433328Multi-class antibodies规格： 48T

Anti-PAX1 配对盒基因1抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Hornerin/S100A18 角化蛋白S100A16抗体 规格 0.2ml

S-100B ELISA Kit 大鼠S100B蛋白 96T

PROCA1 英文名称： Cyclin A1相互作用蛋白抗体 0.2ml

CHMP1A 英文名称： 染色质修饰蛋白1A抗体 0.2ml

Anti-PAX1 配对盒基因1抗体Multi-class antibodies规格： 0.2ml

改良GAM琼脂抑菌剂加入HB8462改良GAM琼脂中

DMEM(高糖)，液体 "含4500mg/l葡萄糖，L-谷氨酰胺 incubation media DMEM(高糖)，液体 "含4500mg/l葡萄糖，L-谷氨酰胺

解脂假丝酵母 与柱花草共生固氮 支/瓶

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0.1%PeptoneBrother

Li-MupirocinMRSMedium,Modified

马铃薯葡萄糖琼脂(PDA) Potato Dextrose Agar 用于霉菌,酵母菌计数(GB标准)

蛋白胨C Peptonized milk 100克 BR

黄豆粉液体培养基250g/瓶用于放线杆菌的培养incubationmedia黄豆粉液体培养基250g/瓶用于放线杆菌的培养

葡萄糖琼脂 250g 用于细菌的综合生化试验

A型核蛋白抗体规格 抗生素检定培养基5号  规格：  100g  用途：  供两性霉素B检定菌种啤酒酵母培养用（中华人民共和国药典2010版）。

抗生素检定培养基4号  规格：  100g  用途：  用于两性霉素B的效价及残留的微生物学检定（中华人民共和国药典2010版）。

抗生素检定培养基3号  规格：  100g  用途：  用于庆大霉素效价的微生物学检定及藤黄八叠球菌菌悬液制备（中华人民共和国药典2010版）。

抗生素检定培养基2号（高PH）  规格：  250g  用途：  用于磷霉素、大观霉素、新霉素、罗红霉素等效价及残留的微生物学检定(中华人民共和国药典2010版...

抗体的生物素化标记实验要点：

1. A型核蛋白抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）A型核蛋白抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。