抗体的生物素化标记实验要点：

1. B淋巴细胞链接激活蛋白抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-NTAL 

中文名称   B淋巴细胞链接激活蛋白抗体费用 

别    名  HSPC046; LAB; Lat2; Linker for activation of B cells; Linker for activation of B-cells; Linker for activation of T cells family member 2; Linker for activation of T cells transmembrane adaptor 2; Linker for activation of T-cells family member 2; Linker of Activated B cells; Membrane associated adapter molecule; Membrane-associated adapter molecule; Non T Cell Activation Linker; Non-T-cell activation linker; NTAL_HUMAN; WBSCR 5; Wbscr15; Williams Beuren syndrome chromosome region 15; Williams Beuren Syndrome chromosome region 5; Williams-Beuren syndrome chromosomal region 15 protein; Williams-Beuren syndrome chromosomal region 5 protein; WSCR5.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human 

产品类型   一抗  

研究领域    细胞生物 免疫学 信号转导

蛋白分子量  predicted molecular weight: 27kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human NTAL 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

B淋巴细胞链接激活蛋白抗体费用 产品应用    ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Non-T cell activation linker (NTAL), a transmembrane adaptor protein, is also designated membrane-associated adapter molecule, Williams-Beuren syndrome chromosome region 15 protein or LAB (linker of activated B cells). NTAL is present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells, and in monocytes and mast cells, but not in resting T lymphocytes. NTAL becomes rapidly tyrosine-phosphorylated upon cross-linking of the B cell receptor (BCR) or of high-affinity Fc g and Fc e receptors of myeloid cells and then associates with the cytoplasmic signaling molecules. NTAL is highly expressed in spleen, lymph node germinal centers and peripheral blood lymphocytes. Defects in the gene encoding for NTAL may cause the musculo-skeletal and cardio-vascular abnormalities that characterize the rare developmental disorder Williams-Beuren syndrome (WBS).

Function : Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.Subunit : When phosphorylated, interacts with GRB2. May also interact with SOS1, GAB1 and CBL.Subcellular Location : Cell membrane. Present in lipid rafts.Tissue Specificity : Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).Post-translational modifications : Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.May be polyubiquitinated.DISEASE : Note=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.

抗体的鉴定：

1）B淋巴细胞链接激活蛋白抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

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Rhesus antibody Rh CST9/Cystatin 9 胱抑素9/半胱氨酸蛋白酶抑制剂9抗体 规格 0.2ml

IL-1 Alpha 白介素1α/IL-1α 0.5mg

Kappa Light Chain 英文名称： k轻链抗体 0.1ml

Rhesus antibody Rh VCP 含缬酪肽蛋白抗体 规格 0.1ml

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ON(Mouse osteonectin) ELISA Kit 小鼠骨粘连蛋白Multi-class antibodies规格： 48T

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Rhesus antibody Rh Donkey Anti-Guinea pig IgG/Alexa Fluor 488 Alexa Fluor 488标记的驴抗豚鼠IgG 规格 0.1ml

Mouse phosphatidylserine,PS ELISA Kit 小鼠磷脂酰丝酸 96T

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Drosophilamedium

DPBS，(1X)，(IVD) "含1000mg/l葡萄糖，36mg/l酸， incubation media DPBS，(1X)，(IVD) "含1000mg/l葡萄糖，36mg/l酸，

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IncompleteAgarMedium

抗生素检定培养基9号250g白色念株菌悬液抗生素效价测定

卵黄氯琼脂培养基基础 250(g) incubation media 卵黄氯琼脂培养基基础 250(g)

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4-甲基伞形葡糖苷酸培养基（MUG培养基）  规格：  100g  用途：  用于大肠埃希氏杆菌快速检测。(《中华人民共和国药典》2010年版)