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蛋白激酶锚定蛋白抗体费用 英文名称  Anti-Neurobeachin

中文名称  蛋白激酶锚定蛋白抗体费用 

别    名 A-kinase anchor protein 550; d AKAP 550; Neurobeachin protein; Rugose protein; AKAP550; NBEA_DROME.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Fruit Fly

产品类型   一抗  

研究领域     细胞生物

蛋白分子量  predicted molecular weight: 395kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from Fruit fly Neurobeachin N-terminus

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

蛋白激酶锚定蛋白抗体费用 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Neurobeachin binds to type II regulatory subunits of protein kinase A and anchors/targets them to the membrane. It may anchor the kinase to cytoskeletal and/or organelle-associated proteins. Neurobeachin, is also a neuron-specific multidomain protein of 327 kDa with a high-affinity binding site for the type II regulatory subunit of protein kinase A. Neurobeachin is peripherally associated with pleomorphic tubulovesicular endomembranes near the trans sides of Golgi stacks and throughout the cell body and cell processes. It is also found in a subpopulation of synapses, where it is concentrated at the postsynaptic plasma membrane.

Function : Binds to type II regulatory subunits of protein kinase A and anchors/targets them to the membrane. May anchor the kinase to cytoskeletal and/or organelle-associated proteins. Required for correct retinal pattern formation and may function in cell fate determination through its interactions with the EGFR and Notch signaling pathways.Subunit : Interacts with RII subunit of PKA and components of the EGFR-mediated and Notch-mediated signaling pathways.Subcellular Location : Cytoplasm. Membrane; Peripheral membrane protein.Tissue Specificity : In early embryos, ubiquitous expression with elevated levels in ventral furrow and flanking mesectodermal cells, neuroblasts and mesoderm. Late embryos show reduced expression in epidermis and skeletal muscle and elevated in nervous system, gut endothelium, tracheal system and salivary gland. Larvae show expression in imaginal disks and many neural cells. Developing eye imaginal disk shows expression throughout the disk and in the region of the morphogenetic furrow. Ubiquitous expression in adults with higher levels in head region.Similarity : Belongs to the WD repeat neurobeachin family.Contains 1 BEACH domain.Contains 5 WD repeats.Database links : UniProtKB/Swiss-Prot: Q9W4E2.3AKAP位于心肌细胞膜浆面L型Ca2+通道旁边，通过锚定PKA，使其能迅速磷酸化Ca2+通道。有实验证明：当阻断PKA与AKMP的结合时，PKA对Ca2+通道的磷酸化作用就被阻断，说明AKMP锚定作用的关键性。

实验流程：

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