抗体的生物素化标记实验要点：

1. T细胞激活核转录因子4抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-NF-ATc4 

中文名称   T细胞激活核转录因子4抗体规格 

别    名  NFATC4 cytoplasmic 4; NF ATc4; NF-AT3; NF-ATc4; NFAC4_HUMAN; NFAT3; NFATc4; Nuclear factor of activated T cells cytoplasmic 4; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 4; Nuclear factor of activated T-cells; nuclear factor of activated T-cells cytoplasmic 4; Nuclear factor of activated T-cells cytoplasmic calcineurin-dependent 4; T cell transcription factor NFAT3; T-cell transcription factor NFAT3.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep 

产品类型   一抗  

研究领域    细胞生物 免疫学 信号转导 淋巴细胞 t-淋巴细胞 表观遗传学

蛋白分子量  predicted molecular weight: 95kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human NF-ATc4 (475-525aa) 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

T细胞激活核转录因子4抗体规格 产品应用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The product of this gene is a member of the nuclearfactors of activated T cells DNA-binding transcription complex.This complex consists of at least two components: a preexistingcytosolic component that translocates to the nucleus upon T cellreceptor (TCR) stimulation and an inducible nuclear component.Other members of this family of nuclear factors of activated Tcells also participate in the formation of this complex. Theproduct of this gene plays a role in the inducible expression ofcytokine genes in T cells, especially in the induction of the IL-2and IL-4. Alternatively spliced transcript variants encodingdifferent isoforms have been noted for this gene.

Function : Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.Subunit : Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers. Interacts with CREBBP, GATA4, IRAK1, MAPK8, MAPK9 and RPS6KA3.Subcellular Location : Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.Tissue Specificity : Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus.Post-translational modifications : Phosphorylated by NFATC-kinases; dephosphorylated bycalcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1,MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and onSer-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.Ubiquitinated, leading to its degradation by the proteasomeand reduced transcriptional activity. Ubiquitination and reductionin transcriptional activity can be further facilitated throughGSK3B-dependent phosphorylation. Polyubiquitin linkage is mainlythrough 'Lys-48'.Similarity : Contains 1 IPT/TIG domain.Contains 1 RHD (Rel-like) domain.Database links : UniProtKB/Swiss-Prot: Q14934.2

抗体的鉴定：

1）T细胞激活核转录因子4抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

CD133 Anti-gen 造血干细胞抗原(多肽抗原)Multi-class antibodies规格： 0.5mg

Anti-CCL11/Eotaxin 1 嗜酸粒细胞趋化蛋白抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh PCDHA3 原钙粘附蛋白α3抗体 规格 0.2ml

IL18R1 Kit Human 人 IL18R1 / CD218a ELISA配对抗体 ELISA

Thyroxine Binding Globulin 英文名称： 甲状腺素结合球蛋白抗体 0.2ml

CD10 英文名称： CD10抗体 0.1ml

Anti-CCL11/Eotaxin 1 嗜酸粒细胞趋化蛋白抗体Multi-class antibodies规格： 0.2ml

CA(Mouse carbonic anhydrase) ELISA Kit 小鼠酶Multi-class antibodies规格： 48T

Anti-TNFRSF13B/TNFRSF14B/TACI/FITC 荧光素标记坏死因子受体超家族成员13B抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Donkey Anti-human IgG 驴抗人IgG 规格 1mg

5-HT (Rabbit 5-Hydroxytryptamine) ELISA Kit 兔子5羟色胺 96T

MLX 英文名称： 转化因子样蛋白4抗体 0.2ml

ARSB 英文名称： 芳基酯酶B抗体 0.2ml

Anti-TNFRSF13B/TNFRSF14B/TACI/FITC 荧光素标记坏死因子受体超家族成员13B抗体IgGMulti-class antibodies规格： 0.2ml

Anti-IL6R Alpha /FITC 荧光素标记白细胞介素6受体α抗体IgGMulti-class antibodies规格： 0.2ml

Anti-p58ipk/FITC 荧光素标记p58ipk抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh BECN1L1 Bcl-2同源结构域样蛋白1抗体 规格 0.2ml

Caspase-1 (ICE; CASP-1;P45) 天冬酸-胱酸特异性蛋白酶家族(抗原) 0.5mg

GPR85 英文名称： G蛋白偶联受体GPR85蛋白抗体 0.2ml

Rhesus antibody Rh RAG1/RNF74 环指蛋白74抗体(重组激活基因1) 规格 0.2ml

Anti-p58ipk/FITC 荧光素标记p58ipk抗体IgGMulti-class antibodies规格： 0.2ml

牛肝颗粒同肝汤配套使用

Fraser Broth Base incubation media Fraser Broth Base

变形杆菌 支/瓶

啶酮酸5.0mg/支*5每支添加于225mlHBJ005中制成LB1肉汤

玫瑰红琼脂 250g 用于霉菌、酵母菌计数

欧洲药典培养基(EP标准)

李斯特菌显色培养基 1000 mL/瓶 incubation media 李斯特菌显色培养基 1000 mL/瓶

李氏增菌液LB1冻干配套试剂 10支 每支添加于225ml(026040)中配成LB1增菌液。

培养基PMediumP(Reinforedmediumforclostridia)用于梭菌的增菌培养和计数。

APTAgar

T细胞激活核转录因子4抗体规格 生化鉴定管  规格：  20支/盒  用途：  细菌生化鉴定

基酸脱羧酶对照生化鉴定管  规格：  20支/盒  用途：  细菌生化鉴定

赖酸脱羧酶生化鉴定管  规格：  20支/盒  用途：  细菌生化鉴定

鸟酸脱羧酶生化鉴定管  规格：  20支/盒  用途：  细菌生化鉴定