抗体的生物素化标记实验要点：

1. 磷酸化2型神经抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-Phospho-NF2(Ser518) 

中文名称   磷酸化2型神经抗体说明书 

别    名  Merlin(Phospho-Ser518); NF2(phospho S518); neurofibromatosis type 2; CAN; BANF; bilateral acoustic neuroma; moesin ezrin radixin like protein; Moesin ezrin radizin like protein; merlin; Neurofibromatosis 2; ACN; Bilateral acoustic neuroma; MERL_HUMAN; Merlin; Moesin ezrin radizin like; Moesin-ezrin-radixin-like protein; Neurofibromatosis type 2; Neurofibromin-2; NF 2; Nf2; Neurofibromatosis2; Neurofibromin 2; Neurofibromin2; Schwannomerlin; NF-2; SCH; Schwannomin.

浓    度   1mg/1ml

规 格  0.1ml/100μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit 

产品类型   一抗 磷酸化抗体  

研究领域     细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 转录调节因子

蛋白分子量  predicted molecular weight: 70kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Merlin around the phosphorylation site of Ser518 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化2型神经抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Neurofibromin 2 (NF2) is a tumor suppressor gene encoding the protein Merlin. Merlin is closely related to the ERM (ezrin, radixin, moesin) family of proteins. The precise funtion of Merlin is not clear. It is thought to provide a link between the actin cytoskeleten and membrane associated proteins, playing a role in transduction of extracellular signals. It has been implicated in cell proliferation and cellular motility. Mutations in the NF2 gene cause neurofibromatosis type II, a condition characterised by the development of tumors in the central nervous system.

Function : Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.Subunit : Interacts with SLC9A3R1, HGS and AGAP2. Interacts with LAYN (By similarity). Interacts with SGSM3. Interacts (via FERM domain) with MPP1. Interacts with WWC1. Interacts with the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex. The unphosphorylated form interacts (via FERM domain) with VPRBP/DCAF1.Subcellular Location : Isoform 1: Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=In a fibroblastic cell line, isoform 1 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with VPRBP in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions.Isoform 7: Cytoplasm, perinuclear region. Cytoplasmic granule. Note=Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 7 is absent from ruffling membranes and filopodia.Isoform 9: Cytoplasm, perinuclear region. Cytoplasmic granule. Note=Observed in cytoplasmic granules concentrated in a perinuclear location. Isoform 9 is absent from ruffling membranes and filopodia.Isoform 10: Nucleus. Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, perinuclear region. Cytoplasmic granule. Cytoplasm, cytoskeleton. Note=In a fibroblastic cell line, isoform 10 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia.Tissue Specificity : Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.Post-translational modifications : Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail.Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation.DISEASE : Neurofibromatosis 2 (NF2) [MIM:101000]: Genetic disorder characterized by bilateral vestibular schwannomas (formerly called acoustic neuromas), schwannomas of other cranial and peripheral nerves, meningiomas, and ependymomas. It is inherited in an autosomal dominant fashion with full penetrance. Affected individuals generally develop symptoms of eighth-nerve dysfunction in early adulthood, including deafness and balance disorder. Although the tumors of NF2 are histologically benign, their anatomic location makes management difficult, and patients suffer great morbidity and mortality. Note=The disease is caused by mutations affecting the gene represented in this entry.Schwannomatosis (SCHWA) [MIM:162091]: Schwannomas are benign tumors of the peripheral nerve sheath that usually occur singly in otherwise normal individuals. Multiple schwannomas in the same individual suggest an underlying tumor-predisposition syndrome. The most common such syndrome is NF2. The hallmark of NF2 is the development of bilateral vestibular-nerve schwannomas; but two-thirds or more of all NF2-affected individuals develop schwannomas in other locations, and dermal schwannomas may precede vestibular tumors in NF2-affected children. There have been several reports of individuals with multiple schwannomas who do not show evidence of vestibular schwannoma. Clinical report suggests that schwannomatosis is a clinical entity distinct from other forms of neurofibromatosis. Note=The disease is caused by mutations affecting the gene represented in this entry.Mesothelioma, malignant (MESOM) [MIM:156240]: An aggressive neoplasm of the serosal lining of the chest. It appears as broad sheets of cells, with some regions containing spindle-shaped, sarcoma-like cells and other regions showing adenomatous patterns. Pleural mesotheliomas have been linked to exposure to asbestos. Note=The disease may be caused by mutations affecting the gene represented in this entry.Similarity : Contains 1 FERM domain.Database links : UniProtKB/Swiss-Prot: P35240.1Ⅱ型神经(neurofibromatosisⅡ,NF2)又称merlin蛋白在细胞与细胞的信号传递中起着关键作用，可能与其它类型也有关联。

抗体的鉴定：

1）磷酸化2型神经抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

CD4(Rabbit Anti-Human Mouse rat CD4 Palyclonal Anti-body) CD4抗原Multi-class antibodies规格： 0.5mg

Anti-Phospho-HP1 gamma (Ser83) 磷酸化染色质相关蛋白1-γ抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh PCDHB15/PCDHB22 原钙粘蛋白15抗体 规格 0.2ml

IL17RA Kit Human 人 IL17RA / CD217 ELISA配对抗体 ELISA

THRA1+2 英文名称： 甲状腺激素受体α1+α2抗体 0.2ml

Collagen I 英文名称： Ⅰ型胶原抗体 0.1ml

Anti-Phospho-HP1 gamma (Ser83) 磷酸化染色质相关蛋白1-γ抗体Multi-class antibodies规格： 0.1ml

DHEA(Mouse Dehydroepiandrosterone) ELISA Kit 小鼠脱氢表雄酮Multi-class antibodies规格： 48T

Anti-CD226/DNAM-1/FITC 荧光素标记CD226抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Donkey Anti-human IgG/Alexa Fluor 488 Alexa Fluor 488标记的驴抗人IgG 规格 0.1ml

AMPK ELISA Kit 鱼磷酸化腺苷酸活化蛋白激酶 96T

MLF1 英文名称： 髓细胞性蛋白1抗体 0.2ml

ADAM17 英文名称： 坏死因子α转换酶 0.1ml

Anti-CD226/DNAM-1/FITC 荧光素标记CD226抗体IgGMulti-class antibodies规格： 0.2ml

HSP-27(Human heat shock protein 27) ELISA Kit 人热休克蛋白27Multi-class antibodies规格： 48T

Anti-P73 protein P73抑制基因抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh HSD17B1 羟类固醇脱氢酶17β-HSD抗体 规格 0.1ml

ATG(Human anti-thymocyte globulin) ELISA Kit 人抗胸腺细胞球蛋白 96T

PDCD2L 英文名称： 程序性细胞死亡2样抗体 0.2ml

CKAP1 英文名称： 细胞骨架相关蛋白1/微管蛋白折叠辅助因子B抗体 0.2ml

Anti-P73 protein P73抑制基因抗体Multi-class antibodies规格： 0.1ml

肉膏酵母葡萄糖琼脂250g用于厌氧菌培养

Fluid A incubation media Fluid A

岛青霉 能防治烟草赤星病，对阳性细菌、大肠杆菌、白色假丝酵母无作用。 支/瓶

ColumbiaBloodAgar

CornMeatMedium

改良CCD琼脂基础(mCCD)250g用于弯曲杆菌的分离培养(GB标准)

李斯特氏菌显色培养基 Listera Chromogenic Medium 用于李斯特氏菌的显色培养，单增李斯特氏菌显蓝色，外围有一不透明环

LPM琼脂 LPM Agar 250 用于单增李氏菌选择性分离(加入七叶苷和柠檬酸铁铵)(FDA标准)

3%氯碱性蛋白胨水250g/瓶用于副溶血性弧菌选择性增菌incubationmedia3%氯碱性蛋白胨水250g/瓶用于副溶血性弧菌选择性增菌

DoubleLactoseBileFermen

磷酸化2型神经抗体说明书 菊糖  规格：  20支/盒  用途：  细菌生化鉴定

蕈糖  规格：  20支/盒  用途：  细菌生化鉴定

粘液酸盐质控肉汤  规格：  20支/盒  用途：  细菌生化鉴定

粘液酸盐测试肉汤  规格：  20支/盒  用途：  细菌生化鉴定