抗体的生物素化标记实验要点：

1. 核因子κB抑制蛋白α抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-NFKBIA/IKB alpha 

中文名称   核因子κB抑制蛋白α抗体费用 

别    名  Inhibitor of KB alpha; I kappa B alpha; I(Kappa)B(alpha); IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat 

产品类型   一抗  

研究领域     免疫学 信号转导 转录调节因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 36kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human IKB alpha 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

核因子κB抑制蛋白α抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.

Function : Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.Subunit : Interacts with RELA; the interaction requires the nuclear import signal. Interacts with NKIRAS1 and NKIRAS2. Part of a 70-90 kDa complex at least consisting of CHUK, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with HBV protein X. Interacts with RWDD3; the interaction enhances sumoylation. Interacts (when phosphorylated at the 2 serine residues in the destruction motif D-S-G-X(2,3,4)-S) with BTRC. Associates with the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC; the association is mediated via interaction with BTRC. Part of a SCF(BTRC)-like complex lacking CUL1, which is associated with RELA; RELA interacts directly with NFKBIA. Interacts with PRMT2. Interacts with PRKACA in platelets; this interaction is disrupted by thrombin and collagen. Interacts with HIF1AN.Subcellular Location : Cytoplasm. Nucleus.Tissue Specificity : Highly expressed in lymph node, thymus followed by liver, brain, muscle, kidney, gastrointestinal and reproductive tract.Post-translational modifications : Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.Sumoylated; sumoylation requires the presence of the nuclear import signal.Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.DISEASE : Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.Similarity : Belongs to the NF-kappa-B inhibitor family.Contains 5 ANK repeats.Database links :UniProtKB/Swiss-Prot: P25963.1Entrez Gene: 282291 CowEntrez Gene: 4792 HumanEntrez Gene: 18035 MouseEntrez Gene: 406188 PigEntrez Gene: 25493 RatOmim: 164008 HumanSwissProt: P25963 HumanSwissProt: Q9Z1E3 Mouse转录调节因子（Transcriptin Regulators）

抗体的鉴定：

1）核因子κB抑制蛋白α抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-GABA-AR Alpha1/FITC 荧光素标记γ1氨基受体α1抗体IgGMulti-class antibodies规格： 0.2ml

Anti-NuMA/FITC 荧光素标记核有丝分裂器NuMA蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh BRLF1 立即早期癌基因BRLF1抗体(基因) 规格 0.2ml

Integrin Beta chain (ITG- Beta3/ITGB3) 整合素-β3(抗原) 0.5mg

HRH2 英文名称： 组胺受体H2抗体 0.1ml

Rhesus antibody Rh rhIL-18 重组人白细胞介素18抗体 规格 0.1ml

Anti-NuMA/FITC 荧光素标记核有丝分裂器NuMA蛋白抗体IgGMulti-class antibodies规格： 0.2ml

CD4(Rat Troponin T) ELISA Kit 小鼠CD4分子Multi-class antibodies规格： 48T

Anti-CD2/FITC 荧光素标记CD2抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh E-cadherin/CD324 上皮钙粘附分子抗体 规格 0.1ml

sPLA2 (Rat secreted phospholipase A2,sPLA2) ELISA Kit 大鼠分泌型磷脂酶A2 96T

MTMR2 英文名称： 肌管素相关蛋白2抗体 0.2ml

Angiopoietin 2 英文名称： 生成素2抗体 0.1ml

Anti-CD2/FITC 荧光素标记CD2抗体IgGMulti-class antibodies规格： 0.2ml

Anti-phosphoM-CSF Receptor(Tyr809) /FITC 荧光素标记兔抗人、大、小鼠磷酸化巨噬细胞集落刺激因子受体抗体IgGMulti-class antibodies规格： 0.2ml

Anti-NRP-2/FITC 荧光素标记神经纤毛蛋白-2抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh BNIP1/TRG8 Bcl2相互作用蛋白1抗体(转化相关基因8蛋白) 规格 0.1ml

GLP-2(Glucagon-like peptide-2) 胰素样肽-2(多肽抗原) 0.5mg

HIPK2 英文名称： Fas相互作用蛋白激酶2抗体 0.2ml

Rhesus antibody Rh Reg3 beta 胰岛β细胞生长因子Reg IIIα 抗体 规格 0.2ml

Anti-NRP-2/FITC 荧光素标记神经纤毛蛋白-2抗体IgGMulti-class antibodies规格： 0.2ml

MSRVMedium，Modified

L-BacterialHypertonicSaltBroth

缓冲蛋白胨水(BPW) Buffered Peptone Water 250 用于沙门氏菌前增菌培养，亦可用于李氏菌前增菌培养(GB、SN标准)

pH7.2磷酸盐缓冲液250g/瓶0.03mol/L，用于样品制备和稀释(国家消毒技术规范)incubationmediapH7.2磷酸盐缓冲液250g/瓶0.03mol/L，用于样品制备和稀释(国家消毒技术规范)

RODAC接触皿(TSA)(6.5cm) 10个/包 用于环境、器皿、设备和表面的无菌检测

科玛嘉大肠杆菌显色培养基M0302-0ml/瓶

技术琼脂 (3号琼脂) (LP0013) Oxoid 500g incubation media 技术琼脂 (3号琼脂) (LP0013) Oxoid 500g

鹿皮色曲霉 支/瓶

ModifiedLaurylSulfateTryptoseBroth-VancoMycin

AntibioticAgarNO.2

核因子κB抑制蛋白α抗体费用 亚碲酸肉汤培养基基础 250(g)  incubation media 亚碲酸肉汤培养基基础 250(g)

葡萄球菌增菌肉汤 250(g)  incubation media 葡萄球菌增菌肉汤 250(g)

葡萄球菌选择性琼脂 250(g)  incubation media 葡萄球菌选择性琼脂 250(g)

胺蓝-DNA琼脂 100(g)  incubation media 胺蓝-DNA琼脂 100(g)