α-中连蛋白抗体费用 英文名称  Anti-NF66/alpha Internexin

中文名称  α-中连蛋白抗体费用 

别    名 a-Internexin; Alpha-Internexin; Alpha-Internexin; 66 kDa neurofilament protein; Alpha Inx; INA; Internexin neuronal intermediate filament protein alpha; MGC12702; NEF 5; NEF5; Neurofilament 5 (66kD); Neurofilament 66; Neurofilament 66 tax binding protein; NF 66; NF-66; TXBP 1; TXBP1.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse

产品类型   一抗  

研究领域    神经生物学 干细胞 细胞粘附分子 细胞骨架

蛋白分子量  predicted molecular weight: 55kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human NF-66 C-terminus

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

α-中连蛋白抗体费用 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NFL, NFM and NFH. Neurofilaments may also include peripherin, alpha internexin, nestin and in some cases vimentin. Alpha internexin is a class IV neuronal intermediate filament that is able to self assemble. It is involved in the morphogenesis of neurons and found predominantly in the adult central nervous system. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NFL to form the filamentous backbone to which NFM and NFH attach to form the cross bridges. Antibodies to alpha internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up regulation of alpha internexin during neuronal regeneration.

Function : Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.Subcellular Location : CytoplasmicTissue Specificity : Found predominantly in adult CNS.Post-translational modifications : O-glycosylated (By similarity).Phosphorylated upon DNA damage, probably by ATM or ATR.Similarity : Belongs to the intermediate filament family.

实验流程：

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