抗体的生物素化标记实验要点：

1. 谷氨酸受体3A+3B抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-NMDAR3A + 3B 

中文名称   谷氨酸受体3A+3B抗体说明书 

别    名  Glutamate Receptor Ionotropic N methyl D aspartate 3A; Glutamate Receptor Ionotropic N methyl D aspartate 3B; Glutamate Receptor Ionotropic N-methyl-D-aspartate 3A; Glutamate Receptor Ionotropic N-methyl-D-aspartate 3B; GRIN3A; GRIN3B; NMDA receptor subunit 3A; NMDA receptor subunit 3B; NMDA Type Glutamate Receptor Subunit NR3B Precursor; NMDAR-L; NR3A; NR3B; NMD3B_HUMAN； NMD3A_HUMAN。

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Rabbit 

产品类型   一抗  

研究领域    神经生物学 通道蛋白 细胞膜受体

蛋白分子量  predicted molecular weight: 123+111kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human NMDAR3A + 3B 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

谷氨酸受体3A+3B抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Glutamate receptors mediate most excitatory neurotransmission in the brain and play an important role in neural plasticity, neural development and neuro-degeneration. Ionotropic glutamate receptors are categorized into NMDA receptors and kainate/AMPA receptors, both of which contain glutamate-gated, cation-specific ion channels. Kainate/AMPA receptors co-localize with NMDA receptors in many synapses and consist of seven structurally related subunits designated GluR-1 to 7. The kainate/AMPA receptors are primarily responsible for fast excitatory neurotransmission by glutamate, whereas the NMDA receptors exhibit slow kinesis of Ca2+ ions and a high permeability for Ca2+ ions. One such NMDA receptor, NR3B, is expressed in motor neurons and forms cation channels impermeable to calcium, which can resist many open-channel blockers. NR3B functions in the brain as an excitatory glycine receptor, modifying the normal role of glycine as an inhibitory neurotransmitter.

Function : NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine.Subunit : Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Does not form functional homomeric channels. Found in a complex with GRIN1, GRIN2A or GRIN2B and PPP2CB. Probably interacts with PPP2CB. No complex with PPP2CB is detected when NMDARs are stimulated by NMDASubcellular Location : Cell membrane; Multi-pass membrane protein (By similarity). Cell junction, synapse, postsynaptic cell membrane (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Note=Enriched in postsynaptic plasma membrane and postsynaptic densities. Requires the presence of GRIN1 to be targeted at the plasma membranePost-translational modifications : N-glycosylatedSimilarity : Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR3A/GRIN3A subfamily.Database links : UniProtKB/Swiss-Prot: Q8TCU5.2 UniProtKB/Swiss-Prot: O60391.2

抗体的鉴定：

1）谷氨酸受体3A+3B抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

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Anti-SIP1 运动神经元存活蛋白结合蛋白1抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Goat Anti-rat IgG/FITC FITC标记的羊抗大鼠IgG 规格 0.3ml

IL-33 ELISA Kit (human) 人白介素23 96T

Notch 2 英文名称： 跨膜受体蛋白Notch-2抗体 0.1ml

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Anti-SIP1 运动神经元存活蛋白结合蛋白1抗体Multi-class antibodies规格： 0.1ml

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Rhesus antibody Rh DRK1/Kv2.1 通道蛋白DRK1抗体 规格 0.2ml

GCP-2/CXCL6(Human granulocyte chemotactic protein-2,GCP-2) ELISA Kit 人粒细胞趋化蛋白-2 96T

Moesin 英文名称： 膜突蛋白抗体 0.1ml

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Anti-CD44/RBITC 红色荧光素罗丹明标记CD44抗体IgGMulti-class antibodies规格： 0.2ml

ADM(Human adrenomedullin) ELISA Kit 人肾上腺髓质素Multi-class antibodies规格： 48T

Anti-ox-LDL 氧化抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh KNDC1 脑蛋白9抗体 规格 0.2ml

IL-4 ELISA KIT 大鼠白介素4 96T

PSD93 英文名称： 突触后密度蛋白93抗体 0.2ml

CSN7b 英文名称： 信号转导蛋白9复合体7b抗体 0.2ml

Anti-ox-LDL 氧化抗体Multi-class antibodies规格： 0.2ml

抗生素检定培养基2号(高pH)250g用于林可霉素，克林霉素等效价测定

HC琼脂基础 250 incubation media HC琼脂基础 250

科玛嘉大肠杆O157菌显色培养基 1000ml 此培养基用于分离和鉴定大肠杆菌O157

BLB培养基250用于双歧杆菌的分离培养incubationmediaBLB培养基250用于双歧杆菌的分离培养

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抗生素检定培养基5号 250(g) incubation media 抗生素检定培养基5号 250(g)

MFC培养基 250g 用于水中粪大肠菌群的滤膜法测定(GB/T5057.12-2006)。

改良马丁培养基MartinMedium,Modified用于药品及生物制品霉菌无菌检验

ReinforcedClostridiumAgar

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