产品订购信息：
英文名称  Anti-NET1 

中文名称   去甲肾上腺素转运蛋白/神经递质去甲肾上腺素转运体抗体费用 

别    名  NAT1; NET; NET; NET1; Norepinephrine transporter; SLC6A2; SLC6A5; SLC6A5; Sodium dependent noradrenaline transporter; Solute carrier family 6 (neurotransmitter transporter norepinephrine) member 5; Solute carrier family 6 member 2; Solute carrier family 6 member 2.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Cow, Horse 

产品类型   一抗  

研究领域    心 细胞生物 神经生物学 信号转导 转录调节因子 转运蛋白

蛋白分子量  predicted molecular weight: 65kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human NET1 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

去甲肾上腺素转运蛋白/神经递质去甲肾上腺素转运体抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Catecholamine, a term used for the hormone adrenaline and its sequentially hydroxylated form noradrenaline, is involved in fight or flight responses. Noradrenaline is released from the post ganglionic sympathetic nerve endings and exerts its effects locally in the immediate vicinity of its release. In the CNS, noradrenaline is involved in a number of physiological responses including mood, sleep regulation, alertness and arousal, both cognitive and non-cognitive expression of behaviors, and control of the endocrine and autonomic nervous systems. Peripherally, noradrenaline is present in sympathetic nerve endings and has full control of the sympathetic nervous system. Noradrenaline released from pre-synaptic nerve terminals is reabsorbed (70-90%) by noradrenaline transporters and its biological effects are terminated. The noradrenaline transport via noradrenaline transporters is an active, Na+/Cl- dependent transport process mediated by noradrenaline transporters. Noradrenaline transporters constitute the primary mechanism for inactivation of synaptically released noradrenaline, are targets for multiple antidepressants and psychostimulants, and are deficient in affective and autonomic disorders. In rat brain, noradrenaline transporter is expressed in noradrenergic neuronal somata, axons and dendrites, and hippocampus and cortex, but is absent from epinephrine- and dopamine-containing neurons. At least 13 genetic variations have been reported in the noradrenaline transporter protein that affect noradrenaline re-uptake and concentrations in cerebrospinal fluid in humans. The association between these genetic variations in noradrenaline transporters and several psychiatric and cardiovascular disorders is just emerging. Recently, a single amino acid mutation (hNET-A457P) showed deficiency in noradrenaline transport in an orthostatic intolerance patient. Noradrenaline transporter protein consists of 617 amino acids and has 12 trans-membrane domains, a characteristic feature of many membrane associated solute transporters.

Function : Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.Subunit : Interacts with RHOA in its GTP- and GDP-bound states, and with CDC42 in its GTP-bound state. Interacts with the PDZ 1 domain of BAIAP1.Subcellular Location : Cytoplasm. Nucleus.Tissue Specificity : Widely expressed.Similarity : Contains 1 DH (DBL-homology) domain.Contains 1 PH domain.Database links : Swiss-Prot: Q7Z628.1

Anti-CK15/FITC 荧光素标记细胞角蛋白15抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Integrin avb3/FITC 荧光素标记整合素avb3抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CCL6/C10 嗜酸粒细胞趋化蛋白CCL6抗体 规格 0.2ml

IL-4(Interleukin-4) 白介素4抗原 0.5mg

IKAP 英文名称： IKK复合物相关蛋白抗体 0.1ml

Rhesus antibody Rh SP-D/PSPD 肺表面活性蛋白D抗体 规格 0.1ml

Anti-Integrin avb3/FITC 荧光素标记整合素avb3抗体IgGMulti-class antibodies规格： 0.2ml

LF/LTF(Mouse Lactoferrin) ELISA KIT 小鼠乳铁传递蛋白/乳铁蛋白Multi-class antibodies规格： 48T

Anti-Integrin Beta1/CD29(integrin beta 1)/FITC 荧光素标记整合素β1蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Dynamin 2 酶动力蛋白2抗体 规格 0.1ml

ADV IgM III 腺病毒 IgM Ⅲ型(间接法二步法)

MOS 英文名称： 原癌基因丝酸/苏酸蛋白激酶MOS抗体 0.2ml

beta Adducin 英文名称： 内收蛋白抗体 0.1ml

Anti-Integrin Beta1/CD29(integrin beta 1)/FITC 荧光素标记整合素β1蛋白抗体IgGMulti-class antibodies规格： 0.2ml

AMBP(Human alpha-1-microglobulin/bikunin precursor) ELISA Kit 人α1微球蛋白/bikunin前体Multi-class antibodies规格： 48T

Anti-OVA 鸡卵白蛋白/卵清蛋白抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh IASPP 细胞凋亡ASPP家族的另一个成员抗体 规格 0.1ml

BP180-Ab(Human anti-Bullous Pemphigoid 180 antibody) ELISA Kit 人抗BP180抗体 96T

PLEKHM3 英文名称： 血小板白细胞C激酶底物同源结构域M3抗体 0.2ml

CLK2 英文名称： 细胞分裂周期样激酶2抗体 0.2ml

Anti-OVA 鸡卵白蛋白/卵清蛋白抗体Multi-class antibodies规格： 0.1ml

SodiumChlorideBloodAgarBase

HelicobacterPyloriMedium

GN 增菌液 Gram Negative Enrichment Broth 250 主要用于志贺氏菌的增菌培养，亦可用于沙门氏菌增菌培养(GB标准)

结晶紫中性红胆盐葡萄糖琼脂(VRBDA)250g/瓶肠道菌计数琼脂，不含乳糖，用于肠道致病菌的选择性分离和计数(SN、ISO)incubationmedia结晶紫中性红胆盐葡萄糖琼脂(VRBDA)250g/瓶肠道菌计数琼脂，不含乳糖，用于肠道致病菌的选择性分离和计数(SN、ISO)

HB-PE自诱导培养基 250 用于基因工程菌大肠杆菌自诱导蛋白表达培养

ColiformChromogenicMedium

抗生素检定培养基2号(高pH) Antibiotic Agar NO.2 用于林可霉素，克林霉素等效价测定

WS琼脂 Wuhan Salmonella Agar 250克 分离沙门氏菌和志贺氏菌用

肠毒素产毒培养基250用于金黄色葡萄球菌肠毒素产毒试验incubationmedia肠毒素产毒培养基250用于金黄色葡萄球菌肠毒素产毒试验

DextroseSemisolidMedium

去甲肾上腺素转运蛋白/神经递质去甲肾上腺素转运体抗体费用 牛胆盐  规格：  100g  用途：  细菌培养基的选择性抑制剂

进口细菌学琼脂粉  规格：  10kg  用途：  培养基凝固剂

进口细菌学琼脂粉  规格：  500g  用途：  培养基凝固剂

技术琼脂粉  规格：  500g  用途：  培养基凝固剂

抗体的生物素化标记实验要点：

1. 去甲肾上腺素转运蛋白/神经递质去甲肾上腺素转运体抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）去甲肾上腺素转运蛋白/神经递质去甲肾上腺素转运体抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。