产品订购信息：
英文名称  Anti-Nm23/NME1/NME2 

中文名称   转移抑制基因抗体规格 

别    名  nucleoside dIPhosphatase kinase A; AWD; AWD, drosophila, homolog of; GAAD; Granzyme A activated DNase; GZMA activated DNase; Metastasis inhibition factor nm23; NB; NBS; NDK A; NDP kinase A; KDKA; NDPK-A; NDPKA; NDPKB; NM23; NM23 H1; NM23 long variant, included; NM23-H1; NM23-M1; NM23B; NM23H1B; NM23H1B, included; NME1; NME1-NME2 spliced read-through transcript, included; NME2; Non metastatic cells 1 protein; Non-metastatic cells 1, protein (NM23A) expressed in; Nonmetastatic cells 1, protein expressed in; Nonmetastatic protein 23; Nonmetastatic protein 23, homolog 1; Nucleoside diphosphate kinase A; Tumor metastatic process associated protein; Tumor metastatic process-associated protein.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow 

产品类型   一抗  

研究领域    

蛋白分子量  predicted molecular weight: 17kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Nm23 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

转移抑制基因抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the nei*oring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Function : Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination.Subunit : Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with SET and PRUNE.Subcellular Location : Cytoplasm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.Tissue Specificity : Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed.Similarity : Belongs to the NDK family.Database links : UniProtKB/Swiss-Prot: P15531.1nm23是一种转移抑制基因，主要用于胃肠道癌、、的多种的研究。大多数研究结果表明，nm23阳性表达与转移负相关，与的预后呈正相关，但也有人研究报道与转移或预后无相关性，说明其结论仍有争议。

MYS ELISA Kit 大鼠肌球蛋白Multi-class antibodies规格： 48T

Anti-5-HTR1A/FITC 荧光素标记5-羟色胺受体1A抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh FOLH1B 细胞生长抑制蛋白26抗体(特异性膜抗原样蛋白) 规格 0.2ml

IL-5(Human Interleukin 5) ELISA KIT 人白介素-5 96T

phospho-MAP4(Ser1073) 英文名称： 磷酸化微管相关蛋白4抗体 0.1ml

ADORA2B 英文名称： 腺苷A2b受体/神经生长因子1受体抗体 0.2ml

Anti-5-HTR1A/FITC 荧光素标记5-羟色胺受体1A抗体IgGMulti-class antibodies规格： 0.2ml

ADRA1A(Mouse adrenergic alpha-1A receptor) ELISA Kit 小鼠能a1A受体Multi-class antibodies规格： 48T

Anti-CCL12/MCP-5/FITC 荧光素标记单核细胞趋化蛋白5抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh EFHC2 EFHC2蛋白抗体 规格 0.2ml

CTX-2 ELISA Kit 小鼠骨退化特异标志物 96T

MPO 英文名称： 髓过氧化物酶抗体 0.1ml

ATP5E 英文名称： ATP5E蛋白抗体 0.2ml

Anti-CCL12/MCP-5/FITC 荧光素标记单核细胞趋化蛋白5抗体IgGMulti-class antibodies规格： 0.2ml

Anti-GLUT3/FITC 荧光素标记葡萄糖转运蛋白3抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Os05g/0477200 protein /FITC 荧光素标记抗水稻白叶枯病菌Os05g0477200蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Biglycan 骨/软骨蛋白多糖1抗体 规格 0.2ml

EGFR- V III 人表皮生长因子受体-8(多肽片断抗原) 0.5mg

HDAC8 英文名称： 组蛋白去乙酰化酶8抗体 0.1ml

Rhesus antibody Rh Rat secretory IgA 分泌型免疫球蛋白A抗体 规格 0.2ml

Anti-Os05g/0477200 protein /FITC 荧光素标记抗水稻白叶枯病菌Os05g0477200蛋白抗体IgGMulti-class antibodies规格： 0.2ml

霉菌培养基(含琼脂水氯霉素)250g用于霉菌的分离培养。

HE琼脂(HE) 250(g) incubation media HE琼脂(HE) 250(g)

XM-G琼脂培养基 XM-G Agar 300克

PALCAM琼脂250用于单增李氏菌选择性分离incubationmediaPALCAM琼脂250用于单增李氏菌选择性分离

Camp-BAPAgarBase,Modified

大肠菌群显色培养基l用于快速、准确检测大肠菌群培养24小时，显兰色

抗生素检定培养基1号(低pH) AntibioticAgar  用于磺苄青霉素效价检定

格蓝氏阴性杆菌增菌液 Gram Negative Enrichment Broth 250克 用于沙门氏菌和志贺菌的增菌培养

7.5%氯肉汤250用于金黄色葡萄球菌的增菌培养(GB标准)incubationmedia7.5%氯肉汤250用于金黄色葡萄球菌的增菌培养(GB标准)

葡萄糖半固体培养基 250g 用于志贺氏菌的复合生化试验(GB标准)

转移抑制基因抗体规格 胰蛋白胨（胰酪胨）  规格：  BR 400g  用途：  培养基原材料

细菌学蛋白胨  规格：  BR 400g  用途：  检测微生物的培养基原材料

细菌学蛋白胨  规格：  250g  用途：  培养基原材料

  规格：  微生物检测配套试剂  用途：

抗体的生物素化标记实验要点：

1. 转移抑制基因抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）转移抑制基因抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。