产品订购信息：
英文名称  Anti-NOS-3/eNOS 

中文名称   一氧化氮合成酶-3（内皮型）抗体说明书 

别    名  cNOS; eNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig 

产品类型   一抗  

研究领域    神经生物学

蛋白分子量  predicted molecular weight: 133kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human NOS-3 C-terminus 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

一氧化氮合成酶-3（内皮型）抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).

eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.Function : Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.Subunit : Homodimer. Interacts with NOSIP and NOSTRIN.Subcellular Location : Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.Tissue Specificity : Platelets, placenta, liver and kidney.Post-translational modifications : Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity.Similarity : Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.Database links :UniProtKB/Swiss-Prot: P29474.3 humanUniProtKB/Swiss-Prot: Q62600.4 ratUniProtKB/Swiss-Prot: P70313.4 mouseEntrez Gene: 287024 CowEntrez Gene: 403784 DogEntrez Gene: 4846 HumanEntrez Gene: 18127 MouseEntrez Gene: 397557 PigEntrez Gene: 24600 RatOmim: 163729 HumanSwissProt: P29473 Cow合成与降解（Synthesis and Degradation)催化生物体内一氧化氮（NO）生成的酶。分神经型一氧化氮合成的酶（nNOS or NOS-1）、诱导型一氧化氮合成的酶（iNOS or NOS-2）、内皮型一氧化氮合成的酶（eNOS or NOS-3）。此抗体识别分子量为135kDa的内皮型一氧化氮合成的酶。

Anti-Aurora A/Aurora B/Aurora C /FITC 荧光素标记兔抗人、大、小鼠有丝分裂激酶A、B、C抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-GCN2 (Thr898) /FITC 荧光素标记兔抗人、大、小鼠磷酸化蛋白激酶GCN2蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CLDND1 膜蛋白CLDND1抗体 规格 0.1ml

IL-6(Interleukin-6) 白介素6(白细胞介素-6)(多肽抗原) 0.5mg

Integrin beta 1 英文名称： 整合素β1/Integrin β1抗体 0.1ml

Rhesus antibody Rh TRIM47 星形细胞瘤高表达蛋白抗体(环指蛋白100) 规格 0.2ml

Anti-Phospho-GCN2 (Thr898) /FITC 荧光素标记兔抗人、大、小鼠磷酸化蛋白激酶GCN2蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Flt3L ELISA Kit 大鼠FMS样酪氨酸激酶3配体Multi-class antibodies规格： 48T

Anti-Apo-E/FITC 荧光素标记载脂蛋白E抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh FAM50C FAM50C蛋白抗体 规格 0.2ml

LBP ELISA Kit 大鼠脂多糖结合蛋白 96T

MPP7 英文名称： 棕榈酰化膜蛋白7抗体 0.1ml

ABCG5 英文名称： 三磷酸腺苷结合转运蛋白G超家族成员5抗体 0.2ml

Anti-Apo-E/FITC 荧光素标记载脂蛋白E抗体IgGMulti-class antibodies规格： 0.2ml

MMP-13(Human Matrix metalloproteinase 13) ELISA kit 人基质金属蛋白酶13Multi-class antibodies规格： 48T

Anti-OPN 骨桥蛋白抗体(分泌型磷蛋白1)人Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh ICAM5/Telencephalin 细胞间粘附分子5抗体 规格 0.1ml

TSH(Mouse Thyroid Stimulating Hormone)ELISA Kit 小鼠促甲状腺素 96T

PCDH8 英文名称： 原钙粘附蛋白8抗体 0.2ml

C17orf66 英文名称： 17号染色体开放阅读框66抗体 0.2ml

Anti-OPN 骨桥蛋白抗体(分泌型磷蛋白1)人Multi-class antibodies规格： 0.1ml

营养肉汤培养基(中国药典)250g用于药品中一般细菌的增菌培养

Hugh-Leifon葡萄糖肉汤(HLGB) 250(g) incubation media Hugh-Leifon葡萄糖肉汤(HLGB) 250(g)

胰酶牛肉汤 胰酶牛肉汤 1万毫升/袋 快疫疫苗用

碱性胆盐琼脂250用于分离霍乱弧菌incubationmedia碱性胆盐琼脂250用于分离霍乱弧菌

盐肉汤 250g 用于蜡样芽孢杆菌盐还原试验(SN标准)

血琼脂培养基基础24060250g加入脱纤维羊血或兔血，制成血琼脂培养基，用于营养要求较高的细菌的培养及溶血试验(GB4789..

卡里-布莱尔转运培养基 (CM0519) Oxoid incubation media 卡里-布莱尔转运培养基 (CM0519) Oxoid

苏云金芽孢杆菌戈尔斯德变种 模式菌株 支/瓶

m-TEC琼脂250g用于水中耐热细菌滤膜计数

MH肉汤(MHB) 250g 用于抗生素敏感试验

一氧化氮合成酶-3（内皮型）抗体说明书 PYG液体培养基冻干配套试剂  规格：  2种*5支/盒  用途：  试剂A和试剂B各一支添加于100ml (027340)PYG液体培养基基础

胰月示-亚盐-环丝酸培养基冻干配套试剂  规格：  10支/盒  用途：  每支添加于100ml（024045）中配成胰月示-亚盐-环丝酸培养基

纤维二糖多粘菌素E（CC）琼脂基础冻干配套试剂  规格：  10支/盒  用途：  每支添加于50ml 025110改良纤维二糖多粘菌素E（CC）琼脂基础

改良纤维二糖多粘菌素B多粘菌素E（mCPC）琼脂基础 冻干配套试剂  规格：  10支/盒  用途：  每支添加于50ml 025110改良纤维二糖多粘菌素B多粘菌素E（mCPC）琼脂基础

抗体的生物素化标记实验要点：

1. 一氧化氮合成酶-3（内皮型）抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）一氧化氮合成酶-3（内皮型）抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。