中文名称  神经生长因子受体相关蛋白1抗体说明书 

英文名称  Anti-NADE/NGFRAP1

别    名 BEX3; BEX3_HUMAN; Brain expressed X linked 3 (mouse) homolog; Brain-expressed X-linked protein 3; Nerve growth factor receptor (TNFRSF16) associated protein 1; Nerve growth factor receptor associated protein 1; Nerve growth factor receptor-associated protein 1; NGFRAP1; Ovarian granulosa cell 13.0 kDa protein HGR74; Ovarian granulosa cell protein; p75NTR associated cell death executor; p75NTR-associated cell death executor; Protein BEX3.

产品属性：

神经生长因子受体相关蛋白1抗体说明书 浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit

产品类型   一抗  

研究领域    细胞生物 神经生物学 细胞凋亡 细胞膜受体

蛋白分子量  predicted molecular weight: 13kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human NADE/NGFRAP1

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

神经生长因子受体相关蛋白1抗体说明书 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 May be a signaling adapter molecule involved in p75NTR-mediated apoptosis induced by NGF. Plays a role in zinc-triggered neuronal death (By similarity). May play an important role in the pathogenesis of neurogenetic diseases.

Tissue specificity: Found in ovarian granulosa cells, testis, prostate and seminal vesicle tissue. High levels also detected in liver.Function : May be a signaling adapter molecule involved inp75NTR-mediated apoptosis induced by NGF. Plays a role inzinc-triggered neuronal death (By similarity). May play animportant role in the pathogenesis of neurogenetic diseases.Subunit : Self-associates. Interacts with 14-3-3 epsilon (YWHAE).Interacts with DIABLO/SMAC (By similarity). Binds to the DEATHdomain of p75NTR/NGFR.Subcellular Location : Nucleus. Cytoplasm. Note=Shuttles betweenthe cytoplasm and the nucleus. Associates with replicatingmitochondria (By similarity).Tissue Specificity : Found in ovarian granulosa cells, testis,prostate and seminal vesicle tissue. High levels also detected inliver.Post-translational modifications : Ubiquitinated. Degraded by the proteasome (By similarity).Similarity : Belongs to the BEX family.Database links : UniProtKB/Swiss-Prot: Q00994.1

实验流程：

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技术外包服务:

★神经生长因子受体相关蛋白1抗体说明书 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

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