产品订购信息：
英文名称  Anti-OTX2 

中文名称   转录因子OTX2抗体费用 

别    名  CPHD6; Homeobox protein OTX2; MCOPS 5; MCOPS5; MGC45000; Orthodenticle 2; Orthodenticle homeobox 2; Orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Orthodenticle2; Otx 2; otx2; OTX2_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Sheep 

产品类型   一抗  

研究领域    发育生物学 神经生物学 干细胞 环指蛋白

蛋白分子量  predicted molecular weight: 32kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human OTX2 (15-105aa) 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

转录因子OTX2抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Transcription factors, OTX1 and OTX2, are two murine homologs of the Drosophila orthodenticle (OTD), show a limited amino acid sequence divergence. OTX1 and OTX2 play an important role during early and later events required for proper brain development in that they are involved in the processes of induction, specification and regionalization of the brain. OTX1 is involved in corticogenesis, sensory organ development and pituitary functions, while OTX2 is necessary earlier in development, for the correct anterior neural plate specification and organization of the primitive streak. OTX2 is also required in the early specification of the neuroectoderm, which is destined to become the fore-midbrain, and both OTX1 and OTX2 co-operate in patterning the developing brain through a dosage-dependent mechanism. A molecular mechanism depending on a precise threshold of OTX proteins is necessary for the correct positioning of the isthmic region and for anterior brain patterning. The genes which encode OTX1 and OTX2 map to human chromosomes 2p13 and 14q21-q22, respectively.

Function : Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

Subcellular Location : Nucleus.

Tissue Specificity : Expressed in brain.

DISEASE : Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures.

Similarity : Belongs to the paired homeobox family. Bicoid subfamily.

Contains 1 homeobox DNA-binding domain.

Database links : UniProtKB/Swiss-Prot: P32243.1

Anti-APEX1/Ref-1 /FITC 荧光素标记氧化还原因子1抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-PLC gamma 1 (Ser1248)/FITC 荧光素标记磷酸化磷酯酶Cγ1抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh COMMD10 铜代谢结构域蛋白10抗体 规格 0.2ml

TPO(Thyroid Peroxidase) 甲状腺过氧化物酶(抗原) 0.5mg

JHD1 英文名称： JmjC结构域组蛋白去甲基化酶H3-K36抗体 0.1ml

Rhesus antibody Rh Tuberin/TSC2 马铃薯球蛋白(性硬化)抗体 规格 0.2ml

Anti-Phospho-PLC gamma 1 (Ser1248)/FITC 荧光素标记磷酸化磷酯酶Cγ1抗体IgGMulti-class antibodies规格： 0.2ml

Survivin 细胞凋亡抑制因子的一种(抗原)Multi-class antibodies规格： 0.5mg

Anti-CD82/KAI1 转移抑制蛋白1抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh OVCA1 相关基因1抗体 规格 0.2ml

CFH Kit Human 人 Complement Factor H / CFH ELISA配对抗体 ELISA

TNFR1 英文名称： 坏死因子受体1抗体 0.1ml

Phospho-CRMP2 (Thr514+Ser518) 英文名称： 磷酸化二氢嘧啶酶样2抗体 0.1ml

Anti-CD82/KAI1 转移抑制蛋白1抗体Multi-class antibodies规格： 0.1ml

IL-2 ELISA KIT 大鼠白介素2Multi-class antibodies规格： 48T

Anti-BAD(phospho Ser75,Ser99,Ser118) /FITC 荧光素标记磷酸化相关死亡促进因子抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ETHE1 乙基脑病蛋白抗体 规格 0.2ml

HSP47 ELISA Kit 大鼠热休克蛋白47 96T

MYPT1 英文名称： 肌球蛋酸酶抗体 0.1ml

Amylin 英文名称： 相关肽/胰岛淀粉样肽抗体 0.1ml

Anti-BAD(phospho Ser75,Ser99,Ser118) /FITC 荧光素标记磷酸化相关死亡促进因子抗体IgGMulti-class antibodies规格： 0.2ml

PGF Protein Mouse 重组小鼠 PIGF / PLGF 蛋白

HL-60 人原髓细胞细胞

F9 Others Mouse 小鼠 Coagulation Factor IX / FIX / F9 人细胞裂解液 (阳性对照)

COLO 320DM 人结直肠腺癌细胞 COLO 320DM human colorectal adenocarcinoma cells RPMI-1640(GIBCO)+10%FBS

恒河猴胚肾细胞;FRhK-4 [FRhK4]

大鼠周神经成纤维细胞(RPNF)(5×105) HEC-1A, 人细胞系 Human

CL-0383MDA-MB-436(人癌细胞)5×106cells/瓶×2

CXADR Others Mouse 小鼠 CXADR / CAR 人细胞裂解液 (阳性对照)

人角膜上皮细胞总RNAHCEpiC NA

ZR75-1细胞，人癌细胞 129小鼠ES细胞,D3细胞 WM35, 人细胞

MV3(人细胞) 5×106cells/瓶×2

HUAEC Pellet 人脐动脉内皮细胞团块 > 1 mio.cells 神经细胞培养基NM-prf

转录因子OTX2抗体费用 PGF Protein Mouse 重组小鼠 PIGF / PLGF 蛋白

HL-60 人原髓细胞细胞

F9 Others Mouse 小鼠 Coagulation Factor IX / FIX / F9 人细胞裂解液 (阳性对照)

COLO 320DM 人结直肠腺癌细胞 COLO 320DM human colorectal adenocarcinoma cells RPMI-1640(GIBCO)+10%FBS

恒河猴胚肾细胞;FRhK-4 [FRhK4]

大鼠周神经成纤维细胞(RPNF)(5×105) HEC-1A, 人细胞系 Human

抗体的生物素化标记实验要点：

1. 转录因子OTX2抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）转录因子OTX2抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。