中文名称  多胺调制因子结合蛋白1抗体说明书 

英文名称  Anti-PMFBP1

别    名 PMF 1 binding protein; PMF-1-binding protein; PMFBP_HUMAN; Pmfbp1; Polyamine modulated factor 1 binding protein 1; Polyamine-modulated factor 1-binding protein 1.

产品属性：

多胺调制因子结合蛋白1抗体说明书 浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheepd

产品类型   一抗  

研究领域    细胞生物 发育生物学 干细胞

蛋白分子量  predicted molecular weight: 119kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from Human PMFBP1

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

多胺调制因子结合蛋白1抗体说明书 产品应用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 PMF-1 is a 205 amino acid protein involved in kinetochore formation. Localized to the nucleus, PMF-1 contains a coiled-coil domain which interacts with the leucine-zipper domain of Nrf2. This interaction regulates the transcription of SSAT, a regulatory enzyme for polyamine catabolism. PMF-1 is also a component of the MIS12 complex, which is required for kinetochore formation and chromosomal alignment and segregation. PMF-1 is expressed at highest levels in skeletal muscle and heart, with moderate expression in liver and kidney. PMFBP1 (Polyamine-modulated factor 1-binding protein 1) is a 1022 amino acid protein that binds PMF-1 and may be involved in general organization of the cytoskeleton. Due to evidence that PMFBP1 may play a role in sperm tail morphology, it may therefore affect fertility. There are three isoforms of PMFBP1 that are produced as a result of alternative splicing events.

Function : May play a role in sperm morphology especially the sperm tail and consequently affect fertility. May also be involved in the general organization of cellular cytoskeleton.Target information above from: UniProt accession Q8TBY8 The UniProt Consortium The Universal Protein Resource (UniProt) in 2010 Nucleic Acids Res. 38:D142-D148 (2010).

Database links : UniProtKB/Swiss-Prot: Q8TBY8.1

实验流程：

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技术外包服务:

★多胺调制因子结合蛋白1抗体说明书 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

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