抗体的生物素化标记实验要点：

1. 磷脂酰肌醇激酶PIK3-γ抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-PIK3 gamma 

中文名称   磷脂酰肌醇激酶PIK3-γ抗体品牌 

别    名  PK3CG_HUMAN; PI 3 Kinase catalytic subunit gamma; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform; PI3-kinase subunit gamma; PI3K-gamma; PI3Kgamma; PtdIns-3-kinase subunit gamma; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma; PtdIns-3-kinase subunit p110-gamma; p110gamma; Phosphoinositide-3-kinase catalytic gamma polypeptide; Serine/threonine protein kinase PIK3CG; 1 phosphatidylinositol 3 kinase; p120-PI3K; p120 PI3K; 5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma; 5-bisphosphate 3-kinase catalytic subunit gamma isoform; p110 gamma; Phosphatidylinositol 3 kinase catalytic 110 kD gamma; Phosphatidylinositol 3 kinase gamma, p110 gamma; Phosphatidylinositol 3 kinase, catalytic, gamma polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit gamma; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit gamma isoform; Phosphatidylinositol-4; Phosphoinositide 3 kinase catalytic gamma polypeptide; Phosphoinositide 3 kinase gamma catalytic subunit; PI3-kinase subunit gamma; PI3CG; PI3K; PIK3; Pik3cg; PtdIns-3-kinase subunit gamma; PtdIns-3-kinase subunit p110-gamma; Serine/threonine protein kinase PIK3CG.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Rabbit 

产品类型   一抗  

研究领域     免疫学 信号转导 激酶和磷酸酶 G蛋白偶联受体 G蛋白信号

蛋白分子量  predicted molecular weight: 121kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PIK3 gamma 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

磷脂酰肌醇激酶PIK3-γ抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. [provided by RefSeq, Jul 2008].

Function : Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Links G-protein coupled receptor activation to PIP3 production. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in T-lymphocyte migration. Regulates T-lymphocyte proliferation and cytokine production. Together with PIK3CD participates in T-lymphocyte development. Required for B-lymphocyte development and signaling. Together with PIK3CD participates in neutrophil respiratory burst. Together with PIK3CD is involved in neutrophil chemotaxis and extravasation. Together with PIK3CB promotes platelet aggregation and thrombosis. Regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism. May have also a lipid kinase activity-dependent function in platelet aggregation. Involved in endothelial progenitor cell migration. Negative regulator of cardiac contractility. Modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels. Regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to ADRBK1 and by non-muscle tropomyosin phosphorylation. Also has serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. May also have a scaffolding role in modulating cardiac contractility. Contributes to cardiac hypertrophy under pathological stress. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.

Subunit : Heterodimer of a catalytic subunit PIK3CG and a PIK3R5 or PIK3R6 regulatory subunit. Interacts with ADRBK1 through the PIK helical domain. Interaction with ADRBK1 is required for targeting to agonist-occupied receptor. Interacts with PDE3B. Interacts with TPM2. Interacts with EPHA8; regulates integrin-mediated cell adhesion to substrate. Interacts with HRAS1; the interaction is required for membrane recruitment and beta-gamma G protein dimer-dependent activation of the PI3K gamma complex PIK3CG:PIK3R6 (By similarity).

Subcellular Location : Cytoplasm. Cell membrane.

Similarity : Belongs to the PI3/PI4-kinase family.

Contains 1 C2 PI3K-type domain.

Contains 1 PI3K-ABD domain.

Contains 1 PI3K-RBD domain.

Contains 1 PI3K/PI4K domain.

Contains 1 PIK helical domain.

Database links : UniProtKB/Swiss-Prot: P48736.3

抗体的鉴定：

1）磷脂酰肌醇激酶PIK3-γ抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

TSGF ELISA Kit 大鼠特异生长因子/相关因子Multi-class antibodies规格： 48T

Anti-TSARG4 生精细胞凋亡相关基因4抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh GHDC GHDC蛋白抗体 规格 0.2ml

TSGF(Mouse Tumor Specific Growth Facter/tumor supplied group of factor) ELISA KIT 小鼠特异生长因子/相关因子 96T

NDRG4 英文名称： 基因NDRG4抗体 0.2ml

ATPase Na+/ K+ beta 2 英文名称： ATP酶通道蛋白抗体 0.1ml

Anti-TSARG4 生精细胞凋亡相关基因4抗体Multi-class antibodies规格： 0.2ml

TP I (topoisomerase I ) 拓普西异构酶Ⅰ抗原Multi-class antibodies规格： 0.5mg

Anti-phosphor-Caldesmon(Ser789) 磷酸化钙介质素抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh PDHA1/PDH-E1α 酸脱氢酶α1抗体 规格 0.2ml

Bax 浓缩液 0.1ml 进口分装

TRF1 英文名称： 端粒体复制结合因子1抗体 0.1ml

C1orf33 英文名称： 1号染色体开放阅读框33抗体 0.2ml

Anti-phosphor-Caldesmon(Ser789) 磷酸化钙介质素抗体Multi-class antibodies规格： 0.2ml

Anti-Lingo-1/FITC 荧光素标记Nogo受体作用蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Astrocyte/FITC 荧光素标记兔抗人星形胶质细胞抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ADRB2 能受体β2抗体(β2-AR ) 规格 0.1ml

Rabbit Anti-Goat IgM/Gold 胶体金标记的兔抗羊IgM 2ml

GODZ 英文名称： 棕榈酰转移酶GODZ抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-dog IgG/APC APC标记的兔抗狗IgG 规格 0.1ml

Anti-Astrocyte/FITC 荧光素标记兔抗人星形胶质细胞抗体IgGMulti-class antibodies规格： 0.2ml

人骨髓间充质干细胞(hMSCs)

NCI-H524 人小细胞

FST Others Human 人 FST / Follistatin 人细胞裂解液 (阳性对照)

mCF, 小鼠成纤维细胞

293来源病毒包装细胞;ΦA-GP

人;HFL1 人淋巴成纤维细胞完全培养基 100mL

CL-0216SMMC-7721(人细胞)5×106cells/瓶×2

MST4 Others Human 人 MST4 杆状病毒-昆虫细胞裂解液 (阳性对照)

人真皮成纤维细胞-胎儿cDNAHDF-f cDNA

5637细胞，人细胞 人胚,CCC-HPF-1细胞 骨骼肌细胞Many types of cells包装：5 × 105次方(1ml)

狗肾恶性症细胞;DH82

THPO Others Human 人 Thrombopoietin / THPO / TPO / TPO 人细胞裂解液 (阳性对照)

磷脂酰肌醇激酶PIK3-γ抗体品牌 人骨髓间充质干细胞(hMSCs)

NCI-H524 人小细胞

FST Others Human 人 FST / Follistatin 人细胞裂解液 (阳性对照)

mCF, 小鼠成纤维细胞

293来源病毒包装细胞;ΦA-GP

人;HFL1 人淋巴成纤维细胞完全培养基 100mL