产品订购信息：
英文名称  Anti-PHKB 

中文名称   磷酸化酶β抗体规格 

别    名  Phosphorylase B; Phosphorylase kinase B; Phosphorylase kinase beta polypeptide; Phosphorylase kinase beta subunit; DKFZp781E15103; FLJ41698; KPBB_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit 

产品类型   一抗  

研究领域     细胞生物 免疫学 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 124kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PHKB 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化酶β抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.[provided by RefSeq, Feb 2010].

Function : Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.

Subunit : Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

Subcellular Location : Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).

Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.

DISEASE : Defects in PHKB are the cause of glycogen storage disease type 9B (GSD9B) [MIM:261750]; also known as phosphorylase kinase deficiency of liver and muscle (PKD). GSD9B is a metabolic disorder characterized by hepathomegaly, only slightly elevated transaminases and plasma lipids, clinical improvement with increasing age, and remarkably no clinical muscle involvement. Biochemical observations suggest that this mild phenotype is caused by an incomplete holoenzyme that lacks the beta subunit, but that may possess residual activity.

Similarity : Belongs to the phosphorylase b kinase regulatory chain family.

磷酸化酶A是磷酸化酶的活性状态，而磷酸化酶B是其无活性状态。6-磷酸葡萄糖结合磷酸化酶后，磷酸化酶由无活性变为活化态，的加长,之后，又回到原来无活性状态，它充当了个桥梁作用。

LIF ELISA Kit 大鼠抑制因子Multi-class antibodies规格： 48T

Anti-AIMP2/JTV1/FITC 荧光素标记抗AIMP2抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh FBXL3 FBXL3蛋白抗体 规格 0.2ml

UK ELISA Kit 大鼠尿激酶 96T

BBS6 英文名称： 巴德-毕德氏综合征蛋白BBS6抗体 0.2ml

A4GNT 英文名称： α1,4-N-乙酰葡糖胺转移酶α4Gn-T抗体 0.2ml

Anti-AIMP2/JTV1/FITC 荧光素标记抗AIMP2抗体IgGMulti-class antibodies规格： 0.2ml

Eukaryotic aspartyl protease(Rice) 天冬氨酸蛋白酶家族蛋白(水稻蛋白的一种)(抗原)Multi-class antibodies规格： 0.5mg

Anti-CCL25/CCR-9 细胞表面趋化因子受体9抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh PAX6 转录因子Pax6抗体 规格 0.2ml

BAMBI Kit Human 人 BAMBI / NMA ELISA配对抗体 ELISA

TRPV6 英文名称： 钙转运蛋白1抗体 0.1ml

Claudin 5 英文名称： 紧密连接蛋白5抗体 0.1ml

Anti-CCL25/CCR-9 细胞表面趋化因子受体9抗体Multi-class antibodies规格： 0.2ml

Notch1/MOTC(Neurogenic locus notch homolog protein 1) 跨膜受体蛋白Notch-1抗原Multi-class antibodies规格： 0.5mg

Anti-AQP3 水通道蛋白-3抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-ATG4D(Ser467) 磷酸化自噬相关蛋白4D抗体 规格 0.1ml

山羊抗人 IgG(H+L)/HRP 0.1ml 美国Jackson公司分装

VGLL1 英文名称： 转录辅助因子退变样蛋白1抗体 0.2ml

DENND2C 英文名称： MAP激酶激活死亡域蛋白2C抗体 0.2ml

Anti-AQP3 水通道蛋白-3抗体Multi-class antibodies规格： 0.1ml

人羊膜上皮细胞

BRL 大鼠肝细胞

XPNPEP2 Others Human 人 XPNPEP2 / X-Pro aminopeptidase 2 人细胞裂解液 (阳性对照)

CL-0126JAR(人胎盘细胞)5×106cells/瓶×2

人肺微内皮细胞裂解物HPMECL

人正常基质永生化细胞;WPMY-1 人心肌成纤维细胞完全培养基 100mL

CL-0114Hs 578T(人癌细胞)5×106cells/瓶×2

KIR2DL4 Others Human 人 KIR2DL4 / CD158D CHO细胞裂解液 (阳性对照)

人外根壳细胞裂解物HHORSCL

牛源细胞 细胞，HCCLM3细胞 C3细胞，小鼠细胞

人胚肾细胞(SV40T基因修饰);293T/17

COCH Others Human 人 COCH / Cochlin 人细胞裂解液 (阳性对照)

磷酸化酶β抗体规格 人羊膜上皮细胞

BRL 大鼠肝细胞

XPNPEP2 Others Human 人 XPNPEP2 / X-Pro aminopeptidase 2 人细胞裂解液 (阳性对照)

CL-0126JAR(人胎盘细胞)5×106cells/瓶×2

人肺微内皮细胞裂解物HPMECL

人正常基质永生化细胞;WPMY-1 人心肌成纤维细胞完全培养基 100mL

抗体的生物素化标记实验要点：

1. 磷酸化酶β抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）磷酸化酶β抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。