产品订购信息：
英文名称  Anti-p53BP1/53BP1 

中文名称   p53结合蛋白1抗体品牌 

别    名  53 BP1; FLJ41424; MGC138366; p202; p53 binding protein 1; p53 BP1; p53BP1; TP53 BP1; TP53BP1; Tumor protein 53 binding protein 1; Tumor protein p53 binding protein 1; Tumor suppressor p53 binding protein 1; TP53B_HUMAN; tumor protein 53-binding protein, 1.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Dog, Rabbit 

产品类型   一抗  

研究领域     免疫学

蛋白分子量  predicted molecular weight: 213kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human p53BP1/53BP1 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

p53结合蛋白1抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 p53 binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, p53 binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.

Function : May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.

Subunit : Interacts with IFI202A (By similarity). Binds to the central domain of p53/TP53. May form homooligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H4 that has been dimethylated at 'Lys-20' (H4K20me2). Has low affinity for histone H4 containing monomethylated 'Lys-20' (H4K20me1). Does not bind histone H4 containing unmethylated or trimethylated 'Lys-20' (H4K20me3). Has low affinity for histone H3 that has been dimethylated on 'Lys-79'. Has very low affinity for histone H3 that has been monomethylated on 'Lys-79' (in vitro). Does not bind unmethylated histone H3. Interacts with MUM1/EXPAND1. Interacts with CHEK2; modulates CHEK2 phosphorylation at 'Thr-68' in response to infrared. Interacts with MSL1; this interaction may be required for MSL1 DNA repair activity, but not for histone acetyltransferase activity.

Subcellular Location : Nucleus. Chromosome; centromere; kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.

Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.

DISEASE : Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.

Similarity : Contains 2 BRCT domains.

Database links : UniProtKB/Swiss-Prot: Q12888.2

p53结合蛋白1(53BP1)是细胞周期检查点激酶蛋白，是新近发现的DNA损伤修复通路中两个非常重要的蛋白激酶MDC1和53BP1蛋白之一,目前多用于方面的研究。

bFGF-4(Human basic fibroblast growth factor 4) ELISA Kit 人碱性成纤维细胞生长因子4Multi-class antibodies规格： 48T

Anti-GRP94/gp96 葡萄糖调节蛋白94抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh MMP-7 基质金属蛋白酶-7抗体 规格 0.1ml

MMP-8(Human Matrix metalloproteinase 8/Neutrophil collagenase) ELISA kit 人基质金属蛋白酶8/中性粒细胞胶原酶 96T

RASA1 英文名称： Rho GTP酶激活蛋白1/骨肥大综合征相关蛋白抗体 0.2ml

CRELD2 英文名称： 富含半胱酸与表皮生长因子样蛋白2抗体 0.2ml

Anti-GRP94/gp96 葡萄糖调节蛋白94抗体Multi-class antibodies规格： 0.1ml

Substance P 人P物质Multi-class antibodies规格： 48T

Anti-Connexin 43 Cx-43蛋白抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh NHLRC1 肌痉挛相关EPM2蛋白抗体 规格 0.2ml

Human Attacin,Att ELISA Kit 人抗菌肽 96T

TNFAIP2 英文名称： 坏死因子诱导蛋白2(TNFα-IP 2)抗体 0.2ml

Connexin 31.1 英文名称： 间隙连接蛋白31.1抗体 0.1ml

Anti-Connexin 43 Cx-43蛋白抗体Multi-class antibodies规格： 0.1ml

Anti-MAFbx/Fbx32/Atrogin-1/FITC 荧光素标记泛素蛋白连接酶抗体IgGMulti-class antibodies规格： 0.2ml

Anti-TEM 1/CD248/FITC 荧光素标记内涎蛋白/内皮唾液酸蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ADAMTS7(NT) 整合素样金属蛋白酶与凝血酶1型-7抗体 (N端抗体) 规格 0.1ml

Rabbit Anti-chicken IgM/FITC FITC标记的兔抗鸡IgM 0.3ml

GNAT3 英文名称： G蛋白转录因子α3抗体 0.2ml

Rhesus antibody Rh Rabbit anti-chicken IgG whole serum 兔抗鸡IgG抗血清 规格 1ml

Anti-TEM 1/CD248/FITC 荧光素标记内涎蛋白/内皮唾液酸蛋白抗体IgGMulti-class antibodies规格： 0.2ml

大鼠免疫球蛋白E(IgE)ELISA试剂盒 ，英文名： IgE ELISA Kit

人内皮抑素(ES)ELISA检测试剂盒HumanEndostatin,ESELISAKit 96T/48T

鸭生长激素1(GH1)免疫试剂盒 Duck growth hormone growth hormone 1,GH1 ELISA kit

CLIAKitforPACAP(Humanpituitaryadenylatecyclaseactivatingpolypeptide)ELISAKit人垂体腺苷酸环化酶激活肽规格：48T/96T

水中肠球菌(Eerococci)基因荧光定量检测试剂盒20次

ELISAKitLCA/CD45人白细胞共同抗原规格：48T/96T

大鼠不对称二甲基精酸(ADMA)ELISA试剂盒 ，英文名： ADMA ELISA Kit

Mouse p53 (p53) ELISA Kit 小鼠p53(p53)ELISA试剂盒

ELISA 小鼠多巴胺(mouse Dopamine) 48T/96T 进口分装

CLIAKitforLBP(Humanlipolysaccharidebindingprotein)ELISAKit人脂多糖结合蛋白规格：48T/96T

通用型vero毒素大肠杆菌志贺氏毒素-2(VTEC-Shigatoxin2)试剂盒20次

ELISAKitLv/Vn红螯光壳螯虾卵黄脂磷蛋白规格：48T/96T

p53结合蛋白1抗体品牌 大鼠低氧诱导因子1α(HIF-1α)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人内皮型合成酶(eNOS)免疫试剂盒 Human Endothelial niic oxide syhase,eNOS ELISA Kit

MousesubstancePreceptor,SP-RELISAKit小鼠P物质受体(SP-R)ELISA试剂盒规格：96T/48T

冰冻切片组织线粒体复合物I蛋白表达NBT显色光学显微镜检测试剂盒10/20次

HumanplateletmembraneglycoproteinⅣ,GP-ⅣELISA试剂盒人血小板膜糖蛋白Ⅳ(GP-Ⅳ)ELISA试剂盒规格：96T/48T

HumanSomelysin-1,ST1ELISAKit人基质裂解素(ST1)ELISA试剂盒规格：96T/48T

抗体的生物素化标记实验要点：

1. p53结合蛋白1抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）p53结合蛋白1抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。