胸苷磷酸化酶抗体规格 英文名称  Anti-TP/thymidine phosphorylase

中文名称  胸苷磷酸化酶抗体规格 

别    名 ECGF 1; PDEGF; ECGF1; Endothelial cell growth factor 1; Endothelial cell growth factor 1 platelet derived; Gliostatin; hPD ECGF; MNGIE; PD ECGF; PDECGF; PDEGF; Platelet derived endothelial growth factor; TdRPase; TP; TYMP; Thymidine Phosphorylase; TYPH_HUMAN; TdRPase.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human

产品类型   一抗  

研究领域     细胞生物 免疫学 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 50kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Thymidine Phosphorylase

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

胸苷磷酸化酶抗体规格 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 In the presence of inorganic orthophosphate, the platelet-derived endothelial growth factor (PD-ECGF) thymidine phosphorylase (TP) (gliostatin) catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites.

Function : May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.

Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.

Subunit : Homodimer.

Similarity : Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Database links : UniProtKB/Swiss-Prot: P19971.2

胸苷磷酸化酶（TP）是一种分子量为50-55kDa的蛋白，在氟嘧啶转化过程中起重要作用。TP具有生成活性，与血小板源性内皮细胞生长因子的结构和作用相似，因此又被称为相关生长因子。其可能通过生成作用促进癌细胞的生长和转移。

实验流程：

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