产品订购信息：
英文名称  Anti-PER2/Period circadian protein 2 

中文名称   节律蛋白2抗体规格 

别    名  Circadian clock protein PERIOD 2; FASPS; hPER 2; hPER2; KIAA0347; PER 2; PER2; Period 2; Period 2 isoform 1; Period circadian protein 2; Period circadian protein homolog 2; Period homolog 2; Period2; FASPS; PER2_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Cow, Horse 

产品类型   一抗  

研究领域     免疫学 染色质和核信号 神经生物学 信号转导 细胞凋亡

蛋白分子量  predicted molecular weight: 138kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PER2 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

节律蛋白2抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 PER2, a mammalian homologue of the Drosophila period gene, shares a 40% homology with PER1 including the protein dimerization PAS domain. PER2 is a circadian regulator that may act as a transcription factor. It behaves as a negative element in circadian transcriptional loop. PER2 does not appear to bind DNA, suggesting indirect transcriptional inhibition. Expression oscillates with a 24 hour rhythm in the suprachiasmatic nucleus (SCN) and the whole eyes. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between PER1 and PER2 oscillations. The expression rhythms appear to originate from retina.

Function : Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK NPAS2-BMAL1

Subunit : Homodimer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with PER1 and PER3, and through a C-terminal domain, with CRY1 and CRY2. Interaction with CSNK1D or CSNK1E promotes nuclear location of PER proteins. Interacts, via its second PAS domain, with TIMELESS in vitro. Interacts with NFIL3 (By similarity). Interacts with PML (By similarity).

Subcellular Location : Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.

Post-translational modifications : Phosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation.

DISEASE : Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.

Similarity : Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 2 PAS (PER-ARNT-SIM) domains.

Database links : UniProtKB/Swiss-Prot: O15055.2

生物钟基因Per2与, 凋亡有着重要的关联，是机体维持正常节律所必需的基因, Per2在。

SLC/CCL21(Human secondary lymphoid-tissue chemokine) ELISA Kit 人二级淋巴组织趋化因子Multi-class antibodies规格： 48T

Anti-GTP cyclohydrolase 1/GTP-CH-1 三磷酸鸟苷环水解酶抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh MMP19 基质金属蛋白酶18/19 规格 0.2ml

MMP-5(Human matrix metalloproteinase 5) ELISA Kit 人基质金属蛋白酶5 96T

Reelin 英文名称： 络丝蛋白抗体 0.1ml

Claudin 8 英文名称： 紧密连接蛋白8抗体 0.2ml

Anti-GTP cyclohydrolase 1/GTP-CH-1 三磷酸鸟苷环水解酶抗体Multi-class antibodies规格： 0.2ml

Hsp-60(Mouse heat shock protein 60)ELISA Kit 小鼠热休克蛋白60Multi-class antibodies规格： 48T

Anti-CLEC13D/MMR1/CD206/FITC 荧光素标记巨噬细胞甘露糖受体抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh DISC1 (CT) DISC1 C端抗体 规格 0.2ml

Streptavidin/PE PE标记链霉亲和素 0.1ml

LRRTM2 英文名称： 富含亮酸重复跨膜神经元蛋白2抗体 0.2ml

新霉素检测试剂盒 96孔/盒 动物组织/水产/蜂蜜/奶

Anti-CLEC13D/MMR1/CD206/FITC 荧光素标记巨噬细胞甘露糖受体抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-HSL (Ser660)/FITC 荧光素标记磷酸化荷尔蒙敏感脂肪酶抗体IgGMulti-class antibodies规格： 0.2ml

Anti-PIBF/FITC 荧光素标记孕激素诱导阻断因子抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ASM/Acid sphingomyelinase 酸性神经鞘磷脂酶抗体 规格 0.1ml

Donkey Anti-Guinea pig IgG/Cy3 Cy3标记的驴抗豚鼠IgG 0.1ml

GLUT6 英文名称： 葡萄糖转运蛋白6抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-mouse IgG/Gold 胶体金标记的兔抗小鼠IgG 规格 0.5ml

Anti-PIBF/FITC 荧光素标记孕激素诱导阻断因子抗体IgGMulti-class antibodies规格： 0.2ml

大鼠粒细胞集落刺激因子(G-CSF)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人神经丝蛋白H(NF-H)免疫试剂盒 Human NF-H ELISA Kit

MouseHelicobacterpyloriIgG,Hp-IgGELISAKit小鼠幽门螺旋菌IgG(Hp-IgG)ELISA试剂盒规格：96T/48T

Androgeeceptor(抗人;抗兔;抗小鼠;抗大鼠)100微克

Humanmembranecofactorprotein,MCPELISA试剂盒人膜辅蛋白(MCP/CD46)ELISA试剂盒规格：96T/48T

Humanplasminogenactivatorinhibitor1，PAI-1ELISAKit人纤溶酶原激活物抑制因子1(PAI-1)ELISA试剂盒规格：96T/48T

大鼠半乳凝素3(GAL3)ELISA试剂盒 ，英文名： GAL3 ELISA Kit

Rat gonadoopin releasing hormone (GH) ELISA Kit 大鼠激素释放激素(GH)ELISA试剂盒

NudeMouseClaracellprotein,CC16ELISAKit 裸鼠克拉拉细胞蛋白(CC16)ELISA试剂盒 96T/48T 进口分装

CLIAKitforColIII(HumanCollageypeIII)ELISAKIT人Ⅲ型胶原规格：48T/96T

细胞乙酰化组蛋白H4染色质免疫沉淀分析(CHIP)试剂盒10次

RatCiatesyhase,CSELISAKit大鼠柠檬酸合酶(CS)ELISA试剂盒规格：96T/48T

节律蛋白2抗体规格 大鼠胆固醇(CH)ELISA试剂盒 96T/48T 试剂盒 组装/原装

人膜联蛋白Ⅵ(ANX-Ⅵ)免疫试剂盒 Human Annexin Ⅵ,ANX-Ⅵ ELISA Kit

Mousethrombusprecursorprotein,TpPELISAKit小鼠前体蛋白(TpP)ELISA试剂盒规格：96T/48T

钙ATP酶PMCA蛋白表达西方杂交分析试剂盒5次

HumanPoliomyelitisVirus,PV-IgGELISA试剂盒人抗副病毒IgG抗体(ai-PIVIgG)ELISA试剂盒规格：96T/48T

Humaargetedribonuclease,ELISAKit人靶向核糖核酸酶()ELISA试剂盒规格：96T/48T

抗体的生物素化标记实验要点：

1. 节律蛋白2抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）节律蛋白2抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。