抗体的生物素化标记实验要点：

1. PCID1蛋白抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-PCID1 

中文名称   PCID1蛋白抗体费用 

别    名  B5 receptor; Dendritic cell protein; eIF3m; EIF3M_HUMAN; Eukaryotic translation initiation factor 3 subunit M; Fetal lung protein B5; FLJ29030; GA17; hfl B5; hFL-B5; PCI domain containing 1 (herpesvirus entry mediator); PCI domain-containing protein 1; PCID1.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Sheep 

产品类型   一抗  

研究领域    细胞生物 免疫学 细菌及

蛋白分子量  predicted molecular weight: 42kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PCID1 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

PCID1蛋白抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2).

Function : Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2).

Subunit : Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3

Subcellular Location : Cytoplasm (By similarity).

Similarity : Belongs to the eIF-3 subunit M family.

Contains 1 PCI domain.

Database links :

Entrez Gene: 421602 Chicken

Entrez Gene: 10480 Human

Entrez Gene: 98221 Mouse

Entrez Gene: 295975 Rat

Omim: 609641 Human

SwissProt: Q7L2H7 Human

SwissProt: Q99JX4 Mouse

Unigene: 502244 Human

Unigene: 379278 Mouse

抗体的鉴定：

1）PCID1蛋白抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

TAb(Human Thyroid Antibody) ELISA Kit 人甲状腺抗体Multi-class antibodies规格： 48T

Anti-ING1/p33 生长抑制因子基因1抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh LOX-1/OLR1 凝集素样氧化型受体抗体 规格 0.2ml

aFABP(Human adipocyte fatty acid-binding protein) ELISA Kit 人脂肪细胞型脂肪酸结合蛋白 96T

Relaxin 1 英文名称： 松弛肽/松弛素抗体 0.2ml

C12ORF68 英文名称： 12号染色体开放阅读框68抗体 0.2ml

Anti-ING1/p33 生长抑制因子基因1抗体Multi-class antibodies规格： 0.1ml

human-VEGF/ELISA 人内皮生长因子Multi-class antibodies规格： 48T

Anti-Claudin-11 少突胶质细胞跨膜蛋白抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh nNos/NOS-1 合成酶-1抗体(神经型) 规格 0.1ml

Human vitronectin,VNR ELISA Kit 人玻连蛋白/体外粘连蛋白 96T

TREM1 英文名称： 髓系细胞触发受体1抗体 0.2ml

CRTC3 英文名称： CREB调节转录激活因子3抗体 0.2ml

Anti-Claudin-11 少突胶质细胞跨膜蛋白抗体Multi-class antibodies规格： 0.1ml

Anti-LRIG1/FITC 荧光素标记抗LRIG1抗体IgGMulti-class antibodies规格： 0.2ml

Anti-SYPH1/FITC 荧光素标记synphilin-1抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Adenylate kinase 2/AK2 腺苷酸激酶1抗体 规格 0.2ml

Rabbit Anti-Goat IgG/PE-CY5 PE-CY5标记的兔抗羊IgG 0.1ml

GluR1 英文名称： 谷酸受体1抗体 0.1ml

Rhesus antibody Rh Rabbit Anti-chicken IgM/Bio 生物素标记的兔抗鸡IgM 规格 0.1ml

Anti-SYPH1/FITC 荧光素标记synphilin-1抗体IgGMulti-class antibodies规格： 0.2ml

大鼠可溶性样凋亡微弱诱导剂(sTWEAK)ELISA试剂盒 ，英文名： sTWEAK ELISA Kit

人内皮细胞粘附分子1(VCAM-1/CD106)ELISA检测试剂盒HumanVascuolarcelladhesionmolecule1,VCAM-1ELISAkit 96T/48T

双酚A(BPA)免疫试剂盒 Bisphenol A,BPA ELISA Kit

CLIAKitforOPN(HumanOsteopoin)ELISAKit人骨桥素规格：48T/96T

糖果糖精(saccharin)含量高效液相色谱法定量检测试剂盒20次

人癌基因蛋白质p190/bcr-ablELISAKit人癌基因蛋白质p190/bcr-ablELISAKit，48T/96T人癌基因蛋白质p190/bcr-ablELISAKit，48T/96T规格：48T/96T

大鼠白细胞抗原DR(HLA-DR)ELISA试剂盒 ，英文名： HLA-DR ELISA Kit

Lactamase inhibitors (BLI) ELISA Kit 小鼠β内酰胺酶抑制剂(BLI)ELISA试剂盒

ELISA 小鼠Foxp3(mouse Foxp3) 48T/96T 进口分装

CLIAKitforirGH(HumanImmunoreactivegrowthhormone)ELISAKit人免疫反应性生长激素规格：48T/96T

通用型二氧化碳(CO2)浓度酶偶联反应比色法定量检测试剂盒50次

ELISAKitTNF-α大鼠坏死因子α规格：48T/96T

PCID1蛋白抗体费用 大鼠单核细胞趋化蛋白2(MCP-2/CCL8)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人膜联蛋白Ⅴ(ANX-Ⅴ)免疫试剂盒 Human Annexin Ⅴ,ANX-Ⅴ ELISA Kit

MouseThyroidStimulatingHormone,TSHELISAKit小鼠促甲状腺素(TSH)ELISA试剂盒规格：96T/48T

肝脾组织样品固着液(Helly固着液)50毫升

HumanPolymorphonuclearElastase,PMNElastaseELISA试剂盒人多形核白细胞弹性蛋白酶(PMNElastase)ELISA试剂盒规格：96T/48T

Huma-cellspecificGTPase,TgtpELISAKit人T细胞特异性鸟苷三磷酸酶(Tgtp)ELISA试剂盒规格：96T/48T