产品订购信息：
英文名称  Anti-PDZK1 

中文名称   PDZ结构域PDZK1蛋白抗体说明书 

别    名  CAP70; CFTR associated protein of 70 kDa; CFTR associated protein, 70-KD; CFTR-associated protein of 70 kDa; CLAMP; D3Ertd537e; Dietary Pi-regulated RNA-1; Diphor-1; mPDZK1; Na(+)/H(+) exchange regulatory cofactor NHE-RF3; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; Na/Pi cotransporter C-terminal-associated protein; NaPi Cap1; NaPi-Cap1; NaPiCap1; NHERF 3; NHERF-3; NHERF3; NHRF3_HUMAN; OTTHUMP00000015572; PDZ domain containing 1; PDZ domain containing protein 1; PDZ domain-containing protein 1; PDZD1; PDZK1; Sodium hydrogen exchanger regulatory factor 3; Sodium-hydrogen exchanger regulatory factor 3; 1700023D20Rik; 2610507N21Rik; 4921513F16Rik; AI267131; AI314638; AL022680; C terminal linking and modulating protein.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Rabbit, Orangutan 

产品类型   一抗  

研究领域     细胞生物 免疫学 信号转导

蛋白分子量  predicted molecular weight: 57kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PDZK1 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

PDZ结构域PDZK1蛋白抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.

Function : A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function (By similarity).

Subunit : Interacts with PDZK1IP1 and ABCC2. Binds to the C-terminal region of SLC26A3. Interacts via its PDZ1 domain with the C-terminal domain of SCARB1. Forms a heterodimeric complex with SLC9A3R1. Interacts with AKAP2, BCR, CFTR, SLC22A12, SLC22A4, SLC22A5, SLC26A6, SLC9A3R2 and SLC17A1. Component of a complex, composed of PDZK1, SYNGAP1, KLHL17 and NMDA receptors. Interacts (via PDZ1 domain) directly with KLHL17; the interaction is important for integrity of actin cytoskeleton structures in neurons (By similarity).

Subcellular Location : Cytoplasm. Membrane. Associated with peripheral membranes. Localizes to the apical compartment of proximal tubular cells and to sinusoidal liver membranes.

Similarity : Belongs to the NHER family.

Contains 4 PDZ (DHR) domains.

Database links :

Entrez Gene: 5174 Human

Entrez Gene: 59020 Mouse

Entrez Gene: 100008900 Rabbit

Entrez Gene: 65144 Rat

Omim: 603831 Human

SwissProt: Q5T2W1 Human

SwissProt: Q9JIL4 Mouse

SwissProt: Q865P3 Rabbit

SwissProt: Q9JJ40 Rat

Unigene: 444751 Human

Unigene: 482226 Mouse

Unigene: 19842 Rat

IL-23(Human Interleukin 23) ELISA Kit 人白介素23Multi-class antibodies规格： 48T

Anti-CXCL7/NAP-2/PPBP 中性粒细胞趋化蛋白2抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Mouse anti-human IgG ascites 小鼠抗人IgG腹水 规格 1ml

SFT(Human sulfatide) ELISA Kit 人脑苷酯 96T

RelB 英文名称： 核转录因子NFKB-RelB蛋白抗体 0.2ml

Cyclin A1 英文名称： 周期素A1蛋白抗体 0.1ml

Anti-CXCL7/NAP-2/PPBP 中性粒细胞趋化蛋白2抗体Multi-class antibodies规格： 0.2ml

PR(Progestogerone receptor)Human 人孕激素受体Multi-class antibodies规格： 48T

Anti-Claudin 4 紧密连接蛋白4抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh NOC3L/FAD24 脂肪细胞分化因子24 规格 0.2ml

Human Beta Lactoglobulin, Beta-Lg ELISA Kit 人β乳球蛋白 96T

TUBA1C 英文名称： 微管相关蛋白α6抗体 0.1ml

C1orf112 英文名称： 1号染色体开放阅读框112抗体 0.2ml

Anti-Claudin 4 紧密连接蛋白4抗体Multi-class antibodies规格： 0.2ml

Anti-HRG- Beta/FITC 荧光素标记抗HRGβ抗体IgGMulti-class antibodies规格： 0.2ml

Anti-P-HP1/FITC 荧光素标记抗异染色质蛋白1磷酸化抗体(果蝇)IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ASPP1 p53凋亡刺激蛋白1抗体 规格 0.1ml

Donkey Anti-Guinea pig IgG/AP 碱性磷酸酶(AP)标记的驴抗豚鼠IgG 0.1ml

GLUD2 英文名称： 谷酸脱氢酶2抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-mouse IgG/PE-Cy5.5 PE-Cy5.5标记的兔抗小鼠IgG 规格 0.1ml

Anti-P-HP1/FITC 荧光素标记抗异染色质蛋白1磷酸化抗体(果蝇)IgGMulti-class antibodies规格： 0.2ml

大鼠可溶性血小板内皮细胞粘附分子1(sPECAM-1/sCD31)ELISA试剂盒 ，英文名： sPECAM-1/sCD31 ELISA Kit

人内皮细胞生长因子B(VEGF-B)ELISA检测试剂盒HumanVascularEndothelialcellGrowthFactorB,VEGF-BELISAKIT 96T/48T

食蟹猴C肽(C-Peptide)免疫试剂盒 Cynomologus Monkey C-Peptide ELISA Kit

CLIAKitforOPGL(MouseOsteoprotegerinLigand)ELISAKIT小鼠骨保护素配体规格：48T/96T

糖果糖精(saccharin)含量紫外光谱法定量检测试剂盒20次

ELISAKitAGPs人阿拉伯半乳糖蛋白规格：48T/96T

大鼠白细胞介素25(IL-25)ELISA试剂盒 96T/48T 试剂盒 组装/原装

人轮状病毒(RV)IgM 免疫试剂盒 Human Rotavirus,RV IgM ELISA Kit

MouseαN-acetylglucosaminidase,αNAGELISAKit小鼠αN已酰氨基葡糖苷酶(αNAG)ELISA试剂盒规格：96T/48T

石蜡切片组织EGFR蛋白表达NBT显色光学显微镜检测试剂盒10/20次

HumanProteinZELISA试剂盒人蛋白Z(ProteinZ)ELISA试剂盒规格：96T/48T

Humaissueinhibitorsofmetalloproteinase4，TIMP-4ELISAKit人基质金属蛋白酶抑制因子4(TIMP-4)ELISA试剂盒规格：96T/48T

PDZ结构域PDZK1蛋白抗体说明书 大鼠单核细胞趋化蛋白1(MCP-1)ELISA试剂盒 ，英文名： MCP-1 ELISA Kit

Porcine apolipoprotein B100 (apo-B100) ELISA Kit 猪载脂蛋白B100(apo-B100)ELISA试剂盒

ELISA 小鼠抗激素/加压素(mouse AVP/ADH) 48T/96T 进口分装

CLIAKitforLPAb-IgA(HumanLegionellaaibodyIgA)ELISAKit人军团菌抗体IgA规格：48T/96T

通用型DNA克隆试剂盒(不包括内切酶)10次

ELISAKitIL-1鸡白介素1规格：48T/96T

抗体的生物素化标记实验要点：

1. PDZ结构域PDZK1蛋白抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）PDZ结构域PDZK1蛋白抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。