抗体的生物素化标记实验要点：

1. 丝氨酸/苏氨酸蛋白磷酸酶1调节亚基10抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-PNUTS/PPP1R10 

中文名称   丝氨酸/苏氨酸蛋白磷酸酶1调节亚基10抗体说明书 

别    名  CAT 53; CAT53; FB 19; FB19; FB19 protein; MHC class I region proline rich protein CAT53; p99; Phosphatase 1 nuclear targeting subunit; Phosphatase nuclear targeting subunit; PNUTS; PP1 binding protein of 114 kDa; PP1R10; Protein phosphatase 1 regulatory (inhibitor) subunit 10; Protein phosphatase 1 regulatory subunit 10; Serine/threonine protein phosphatase 1 regulatory subunit 10; PP1RA_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Sheep 

产品类型   一抗  

研究领域    神经生物学 信号转导 激酶和磷酸酶 Alzheimer's

蛋白分子量  predicted molecular weight: 99kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PNUTS/PPP1R10 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

丝氨酸/苏氨酸蛋白磷酸酶1调节亚基10抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Eukaryotic protein phosphorylation and dephosphorylation on serine and threonine residues regulates numerous cell functions, including division, homeostasis and apoptosis. A group of proteins that play a major role in this process are the serine/threonine protein phosphatases. Protein phosphatase (PP) holoenzyme is a trimeric complex that contains a regulatory subunit, a variable subunit and a catalytic subunit. Families of PP catalytic subunits include PP1, PP2A, PP2B, PP2C, PPX and PP5. Regulatory subunits include nuclear inhibitor of PP1 (NIPP1), PP1 nuclear targeting subunit (PNUTS), PP2A-A, PP2A-B, PP2A-B56, PP2A-C, PP2B-B and PR48. PNUTS, also designated CAT53 or FB19, is encoded by the gene PPP1R10. PNUTS acts as an inhibitor for the phosphatase activity of PP1 Alpha and PP1 Gamma. It is a nuclear protein primarily detected in nucleoplasmic bodies and within nucleoli. PNUTS expression levels are highest in brain, heart, lung, placenta, liver, kidney, pancreas and skeletal muscle.

Function : PPP1R10 (serine/threonine protein phosphatase 1 regulatory subunit 10), also known as PNUTS, is a targeting subunit of protein phosphatase 1 (PP1). Furthermore, PPP1R10 acts as an inhibitor of PPP1CA and PPP1CC phosphatase activities. Its inhibitory activity on PPP1CA is only observed when PPP1R10 is phosphorylated by Protein Kinase A (PKA). PPP1R10 contains a zinc finger motif and is predominantly localised within the nucleus. Recently, PPP1R10 has been shown to target PP1 to GABA(C) receptors, shuttling from the nucleus to the cell membrane in cells co-expressing this receptor. This highlights a role for this protein in the regulation of synaptic signal transduction. PPP1R10 has also been shown to regulate the activity of p53 in response to hypoxia.

Subunit : Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA or PPP1CB or PPP1CC. Interacts with PPP1CC. Interacts with PPP1CA, WDR82 and TOX4

Subcellular Location : Nucleus. Note=Found in discrete nucleoplasmic bodies and within nucleoli. Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase

Post-translational modifications : Phosphorylated on Ser-398 and Thr-400 by PKA within the region necessary for interaction with PPP1CA

Similarity : Contains 1 C3H1-type zinc finger.

Contains 1 TFIIS N-terminal domain.

Database links : UniProtKB/Swiss-Prot: Q96QC0.1

抗体的鉴定：

1）丝氨酸/苏氨酸蛋白磷酸酶1调节亚基10抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

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Rhesus antibody Rh LIR-8/CD85c/LILRB5 白球蛋白样受体8抗体 规格 0.2ml

PC/CPG(Human choline phosphoglyceride) ELISA Kit 人胆碱磷酸甘油酯 96T

RMND1 英文名称： 减数分裂同源蛋白1抗体 0.2ml

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Rhesus antibody Rh Nucleolar protein 3/Apoptosis repressor with CARD 核仁蛋白3抗体 规格 0.2ml

RNA plant 植物RNA提取的专用试剂 50ml

TBX18 英文名称： 转录因子Tbx18抗体 0.2ml

COL4A3BP 英文名称： IV型胶原α3结合蛋白抗体 0.2ml

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Rabbit Anti-rat IgG/RBITC 罗丹明标记的兔抗大鼠IgG 0.3ml

GIMAP6 英文名称： GTP酶IMAP家族成员6抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-human sIgA/Alexa Fluor 350 Alexa Fluor 350标记的兔抗人分泌型IgA 规格 0.1ml

Rabbit Anti-human sIgA/FITC 荧光素标记兔抗人分泌型IgA抗体Multi-class antibodies规格： 0.2ml

大鼠抗凝血酶Ⅲ抗体(AT-Ⅲ)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

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MouseIerferonβ,IFN-β/IFNBELISAKit小鼠β干扰素(IFN-β/IFNB)ELISA试剂盒规格：96T/48T

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大鼠白介素23(IL-23)ELISA试剂盒 ，英文名： IL-23 ELISA Kit

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ELISA 小鼠IgM(mouse IgM) 48T/96T 进口分装

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ELISAKitAch大鼠乙酰胆碱规格：48T/96T

丝氨酸/苏氨酸蛋白磷酸酶1调节亚基10抗体说明书 大鼠成纤维细胞生长因子15(FGF15)ELISA试剂盒 ，英文名： FGF15 ELISA Kit

Rat leukemia inhibitory factor receptor (LIFR) ELISA Kit 大鼠抑制因子受体(LIFR)ELISA试剂盒

RatSolubleprotein-100B,S-100BELISAKit 大鼠S100B蛋白(S-100B)ELISA试剂盒 96T/48T 进口分装

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