产品订购信息：
英文名称  Anti-PTF1A 

中文名称   *特异转录因子PTF1A抗体品牌 

别    名  PTF1-p48; bHLH transcription factor p48; bHLHa29; Class A basic helix-loop-helix protein 29; Class II bHLH protein PTF1A; Exocrine pancreas specific transcription factor p48; p48 DNA binding subunit of transcription factor PTF1; p48 DNA-binding subunit of transcription factor PTF1; Pancreas specific transcription factor 1a; Pancreas transcription factor 1 subunit alpha; Pancreas-specific transcription factor 1a; PTF 1A; PTF1 p48; PTF1-p48; Ptf1a; PTF1A_HUMAN; PTF1P48.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Pig, Cow 

产品类型   一抗  

研究领域     细胞生物 转录调节因子 表观遗传学

蛋白分子量  predicted molecular weight: 35kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PTF1A 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

*特异转录因子PTF1A抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 PTF1 is a pancreas-specific protein that functions as a component of the trimeric pancreas transcription factor 1 (PTF1) complex. Localizing to the nucleus, PTF1 contains one basic helix-loop-helix (bHLH) domain and is believed to play an important role in mammalian pancreatic development, functioning as a transcription factor that regulates the specification of all three pancreatic cell types. PTF1 interacts with RBP-Jⅹ and, together, they cooperate in regulating the expression of PDX-1 (pancreas/duodenum homeobox protein 1), a key regulator of pancreatic islet development and insulin gene transcription in beta-cells. Loss of functional PTF1 can cause pancreatic progenitors to take on the normal fates of duodenal epithelia. Mutations in the gene encoding PTF1 lead to diabetes mellitus and cerebellar hypoplasia/agenesis, suggesting that PTF1 also plays and important role in cerebellar neurogenesis.

Function : Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells (By similarity). Plays an important role in cerebellar development.

Subcellular Location : Nucleus. Cytoplasm. In chronic pancreatitis associated with pancreas cancer preferentially accumulates in the cytoplasm of acinar/ductular complexes. In the cytoplasm loses its ability to form the PTF1 complex.

Tissue Specificity : Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.

Similarity : Contains 1 basic helix-loop-helix (bHLH) domain.

Database links : UniProtKB/Swiss-Prot: Q7RTS3.1

CD4(Human cluster Of differentiation) ELISA Kit 人CD4分子Multi-class antibodies规格： 48T

Anti-CXCR3/CD183 细胞表面趋化因子受体3抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Mouse Anti-human IgG(3D3)/Gold 胶体金标记的鼠抗人IgG单克隆抗体 规格 0.5ml

Human hypothetical protein LOC677340 ELISA Kit 人假定蛋白LOC677340 96T

RNF12 英文名称： 环指蛋白12抗体 0.2ml

ICAM1 英文名称： 细胞间粘附分子-1抗体 0.1ml

Anti-CXCR3/CD183 细胞表面趋化因子受体3抗体Multi-class antibodies规格： 0.2ml

Gentamicin/FITC 荧光素标记庆大霉素Multi-class antibodies规格： 1ml

Anti-CGP-39/YKL-40 软骨糖蛋白39抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh ODF2/Cenexin1 尾部结构蛋白抗体 规格 0.1ml

Fluorescent Mounting Media 抗荧光淬灭剂 3ml

TIF1 gamma 英文名称： 转录中介因子Tif1γ抗体 0.2ml

CT13 英文名称： /抗原13抗体 0.2ml

Anti-CGP-39/YKL-40 软骨糖蛋白39抗体Multi-class antibodies规格： 0.1ml

Anti-Phospho-NDRG1 (Ser330)/FITC 荧光素标记磷酸化分化相关基因NDRG1抗体IgGMulti-class antibodies规格： 0.2ml

Anti-VEGF-A/FITC 荧光素标记内皮生长因子A抗体IgGMulti-class antibodies规格： 0.2ml

β淀粉样肽1-42 Anti-β-Amyloid 1-42 CT 0.1ml

Mouse Anti-human IgM/Bio 生物素标记的小鼠抗人IgM 0.3ml

GHRHR 英文名称： 生长激素释放因子受体抗体 0.1ml

Rhesus antibody Rh PROK1/PRK1/EG-VEGF 腺衍生内皮生长因子抗体 规格 0.1ml

Anti-VEGF-A/FITC 荧光素标记内皮生长因子A抗体IgGMulti-class antibodies规格： 0.2ml

大鼠抗红细胞抗体(RBC)ELISA试剂盒 ，英文名： RBC ELISA Kit

人白介素1可溶性受体Ⅰ(IL-1sRⅠ)ELISA检测试剂盒Humansolubleierleukin-1receptorⅠ,IL-1sRⅠELISAkit 96T/48T

病毒通用型(FMDV-U)核酸检测试剂盒(-PCR法) 48T

CLIAKitforNON(Humannonmethylatedoligonucleotide0ELISAKit人非甲基化寡核苷酸规格：48T/96T

体外非细胞系统细胞色素P450亚酶CYP2E(AH)活性荧光定量检测试剂盒20次

ELISAKitIDUA人αL艾杜糖苷酸酶规格：48T/96T

大鼠白介素2(IL-2)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人硫酸脑苷酯(SFT)免疫试剂盒 Human sulfatide,SFT ELISA Kit

PlaHumanVitaminC,VCELISAKit植物维生素C(VC)ELISA试剂盒规格：96T/48T

石蜡切片组织PAR-2蛋白表达NBT显色光学显微镜检测试剂盒10/20次

Humanpyruvatekinase,PKELISA试剂盒人同酸激酶(PK)ELISA试剂盒规格：96T/48T

Humani-iodothyronine，T3ELISAKit人三甲状腺原氨酸(T3)ELISA试剂盒规格：96T/48T

*特异转录因子PTF1A抗体品牌 大鼠超氧化物歧化酶(SOD)ELISA试剂盒 ，英文名： SOD ELISA Kit

Mouse C type naiuretic peptide (CNP) ELISA Kit 小鼠C型尿肽(CNP)ELISA试剂盒

ELISA 小鼠羟甲基赖氨酸(mouse CML) 48T/96T 进口分装

CLIAKitforLDL-IC(MouseLDL-IC)ELISAKit小鼠免疫复合物规格：48T/96T

通用型KSHV(KAPOSISARCOMA-ASSOCIATEDHERPESVIRUS)病毒定量PCR扩增检测试剂盒20次

ELISAKitsVCAM-1猴可溶性细胞粘附分子1规格：48T/96T

抗体的生物素化标记实验要点：

1. *特异转录因子PTF1A抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）*特异转录因子PTF1A抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。