产品订购信息：
英文名称  Anti-PLEKHM1 

中文名称   石骨症相关蛋白PLEKHM1抗体品牌 

别    名  162 kDa adapter protein; AP162; PH domain-containing family M member 1; PKHM1_HUMAN; Pleckstrin homology domain-containing family M member 1; PLEKHM1.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Pig, Horse, Rabbit 

产品类型   一抗  

研究领域    细胞生物 免疫学

蛋白分子量  predicted molecular weight: 117kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PLEKHM1 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

石骨症相关蛋白PLEKHM1抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Involved in vesicular transport in the osteoclast (By similarity). May have a role in sialyl-lex-mediated transduction of apoptotic signals.

Tissue specificity: Expressed in placenta, liver, prostate, thymus, spleen, ovary, colon, colon carcinoma and peripheral blood lymphocytes (PBL). Weakly expressed in brain, lung, kidney, and testis. No expression in heart, skeletal muscle, pancreas and small intestine. Predominantly expressed in the breast carcinoma cell line MCF-7.

Involvement in disease:Defects in PLEKHM1 are the cause of osteopetrosis autosomal recessive type 6 (OPTB6); also known as autosomal recessive osteopetrosis intermediate form. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts.

Function : Involved in vesicular transport in the osteoclast (Bysimilarity). May have a role in sialyl-lex-mediated transduction ofapoptotic signals.

Subcellular Location : Cytoplasm (Potential).

Tissue Specificity : Expressed in placenta, liver, prostate,thymus, spleen, ovary, colon, colon carcinoma and peripheral bloodlymphocytes (PBL). Weakly expressed in brain, lung, kidney, andtestis. No expression in heart, skeletal muscle, pancreas and smallintestine. Predominantly expressed in the breast carcinoma cellline MCF-7.

DISEASE : Defects in PLEKHM1 are the cause of osteopetrosisautosomal recessive type 6 (OPTB6) [MIM:611497]; also known asautosomal recessive osteopetrosis intermediate form. Osteopetrosisis a rare genetic disease characterized by abnormally dense bone,due to defective resorption of immature bone. The disorder occursin two forms: a severe autosomal recessive form occurring in utero,infancy, or childhood, and a benign autosomal dominant formoccurring in adolescence or adulthood. Autosomal recessiveosteopetrosis is usually associated with normal or elevated amountof non-functional osteoclasts.

Similarity : Contains 2 PH domains.

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 RUN domain.

Database links : UniProtKB/Swiss-Prot: Q9Y4G2.3

CYFRA21-1(Human cytokeratin fragment antigen 21-1) ELISA Kit 人细胞角蛋白21-1片段Multi-class antibodies规格： 48T

Anti-Phospho-HER3 (Tyr1222) 磷酸化HER3受体抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh MIA2 抑制活性蛋白2抗体 规格 0.2ml

uPA/PLAU(Human urokinase-type plasminogen activator) ELISA Kit 人尿激酶型纤溶酶原激活物 96T

RNF149 英文名称： 环指蛋白149抗体 0.2ml

CEP72 英文名称： 中心体蛋白72抗体 0.1ml

Anti-Phospho-HER3 (Tyr1222) 磷酸化HER3受体抗体Multi-class antibodies规格： 0.1ml

MPO(Mouse myeloperoxidase) ELISA Kit 小鼠髓过氧化物酶Multi-class antibodies规格： 48T

Anti-CEBP- Alpha /CLEBP Alpha /FITC 荧光素标记转录调节因子C/EBP α 抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Donkey Anti-Goat IgG/FITC FITC标记的驴抗羊IgG 规格 0.3ml

IFN- Gamma ELISA Kit(mouse) 小鼠γ干扰素 96T

phospho-LRP5(Thr1492) 英文名称： 磷酸化受体相关蛋白5抗体 0.1ml

Phospho-ATG4C(Ser398) 英文名称： 磷酸化自噬相关蛋白4C抗体 0.1ml

Anti-CEBP- Alpha /CLEBP Alpha /FITC 荧光素标记转录调节因子C/EBP α 抗体IgGMulti-class antibodies规格： 0.2ml

Anti-HDAC10/FITC 荧光素标记组蛋白去乙酰化酶10抗体IgGMulti-class antibodies规格： 0.2ml

Anti-PEG10 /FITC 荧光素标记高表达基因抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh BAALC 脑和急性胞浆蛋白抗体 规格 0.2ml

Actin Alpha /Alpha-SMA (Actin alpha , smooth muscle aorta) 肌动蛋白(抗原) 0.5mg

GGPS1 英文名称： 法尼基二磷酸合酶1抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-pig IgG/Cy3 Cy3标记的兔抗猪IgG 规格 0.1ml

Anti-PEG10 /FITC 荧光素标记高表达基因抗体IgGMulti-class antibodies规格： 0.2ml

大鼠巨噬细胞炎性蛋白3β(MIP-3β/ELC/CCL19)ELISA试剂盒 ，英文名： MIP-3β/ELC/CCL19 ELISA Kit

人白介素6(IL-6)ELISA检测试剂盒HumanIerleukin6,IL-6ELISAKIT 96T/48T

高致病性猪蓝耳病病毒(PRRSV-M)核酸检测试剂盒(-PCR法) 48T

CLIAKitforNKBELISAKit(Ret)大鼠神经肽B规格：48T/96T

体液3-磷酸甘油脱氢酶(GAPDH)活性酶动力比色法定量检测试剂盒20次

ELISAKitTCR人T细胞受体规格：48T/96T

大鼠白介素1β(IL-1B)ELISA试剂盒 ，英文名： IL-1B ELISA Kit

Hamster receptor activator of nuclear factor kappa B ligand (RANKL) ELISA Kit 仓鼠核因子κB受体活化因子配基(RANKL)ELISA试剂盒

MouseypsinELISAKit 小鼠胰蛋白酶(ypsin)ELISA试剂盒 96T/48T 进口分装

CLIAKitforHumanN-Acetyl-Ser-Asp-Lys-Pro,AcSDKPELISAKit人N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸规格：48T/96T

铜离子膜通道转运功能检测试剂盒20次

ELISAKitsCD40L大鼠可溶性白细胞分化抗原40配体规格：48T/96T

石骨症相关蛋白PLEKHM1抗体品牌 大鼠层连蛋白/板层素(LN)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人免疫球蛋白E Fc段受体Ⅱ(FcεRⅡ/CD23)免疫试剂盒 Human Receptor Ⅱ for the Fc region of immunoglobulin E,FcεRⅡ ELISA Kit

MouseVascularEndothelialcellGrowthFactor,VEGFELISAKit小鼠内皮细胞生长因子(VEGF)ELISA试剂盒规格：96T/48T

石蜡切片组织CASPASE-2蛋白表达NBT显色光学显微镜检测试剂盒10/20次

HumanProstaglandinE2,PG-E2ELISA试剂盒人素E2(PGE2)ELISA试剂盒规格：96T/48T

Humahymopein,TP-5ELISAKit人胸腺五肽(TP-5)ELISA试剂盒规格：96T/48T

抗体的生物素化标记实验要点：

1. 石骨症相关蛋白PLEKHM1抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）石骨症相关蛋白PLEKHM1抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。