抗体的生物素化标记实验要点：

1. 区带蛋白PZP抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-Pregnancy zone protein 

中文名称   区带蛋白PZP抗体规格 

别    名  A1m antibody; C3 and PZP like alpha-2 macroglobulin domain containing protein 6; Complement component 3 and pregnancy zone protein like alpha 2 macroglobulin domain containing protein 6; CPAMD6; PZP; PZP_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Cow, Horse, Rabbit 

产品类型   一抗  

研究领域    神经生物学 细胞粘附分子

蛋白分子量  predicted molecular weight: 161kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Pregnancy zone protein 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

区带蛋白PZP抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Pregnancy Zone protein is a 1,482 amino acid secreted protein that belongs to the protease inhibitor I39 family and exists as multiple alternatively spliced isoforms. Expressed predominately in plasma and in late-pregnancy sera, Pregnancy Zone protein functions as a disulfide-linked homotetramer that is able to trap and inhibit proteinases, thus playing a role in the regulation of protein splitting and small peptide formation. The gene encoding Pregnancy Zone protein maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and Trisomy 12p, which causes facial developmental defects and seizure disorders.

Function : Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Subunit : Homotetramer, which consists of two pairs of disulfide-linked chains.

Subcellular Location : Secreted

Database links : UniProtKB/Swiss-Prot: P20742.4

抗体的鉴定：

1）区带蛋白PZP抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

TAP(Human trypsinogen activation peptide) ELISA Kit 人胰蛋白酶原激活肽Multi-class antibodies规格： 48T

Anti-IAA (Indole-3-Acetic Acid) 吲哚乙酸抗体/植物生长素抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh MAPKAP Kinase 2 原活化蛋白激酶活化的蛋白激酶2抗体 规格 0.2ml

UGCG(Human UDP-glucose ceramide glucosyltransferase) ELISA Kit 人尿苷二磷酸葡萄糖神经酰胺葡萄糖基转移酶 96T

ROM1 英文名称： 视网膜感光细胞外节膜蛋白1抗体 0.2ml

CRIPTO 英文名称： 衍化生长因子抗体(N端) 0.2ml

Anti-IAA (Indole-3-Acetic Acid) 吲哚乙酸抗体/植物生长素抗体Multi-class antibodies规格： 0.2ml

NF-L(Neurofilament triplet L) 低分子量神经丝蛋白(抗原)Multi-class antibodies规格： 0.5mg

Anti-CD8 CD8抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh p73 alpha p53相关蛋白P73α抗体 规格 0.2ml

CST6 Kit Human 人 Cystatin E / CST6 ELISA配对抗体 ELISA

T2R6 英文名称： 味觉感受器蛋白T2R6抗体 0.2ml

C21orf55 英文名称： 21号染色体开放阅读框55抗体 0.2ml

Anti-CD8 CD8抗体Multi-class antibodies规格： 0.1ml

Anti-IGF-II/FITC 荧光素标记胰岛素样生长因子-II抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Plexin A2/FITC 荧光素标记神经丛蛋白A2抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ARMCX1 ARM重复X连锁蛋白ARMCX1抗体 规格 0.2ml

Donkey Anti-human IgG/RBITC 罗丹明标记的驴抗人IgG 0.1ml

GCP4 英文名称： γ-微管蛋白GCP4抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-Monkey IgG 兔抗猴IgG 规格 1mg

Anti-Plexin A2/FITC 荧光素标记神经丛蛋白A2抗体IgGMulti-class antibodies规格： 0.2ml

大鼠甲状腺素抗体(TAb)ELISA试剂盒 ，英文名： TAb ELISA Kit

人凋亡相关因子(FAS/CD95)ELISA检测试剂盒HumanFactor-relatedApoptosis,FASELISAKit 96T/48T

猪支原体(MHP)核酸检测试剂盒 48T

CLIAKitforMYOG(Humanmyogenin)ELISAKit人肌细胞生成素规格：48T/96T

体液半胱氨酸(cysteine)含量高效液相色谱法定量检测试剂盒20次

ELISAKitD2D人D二聚体规格：48T/96T

大鼠γ干扰素(IFN-γ)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人磷脂转移蛋白β(PITPNB)免疫试剂盒 Human Phosphatidylinositol ansfer protein beta isoform,PITPNB ELISA kit

Porcineai-thrombieceptor,AELISAKit猪抗凝血酶受体(A)ELISA试剂盒规格：96T/48T

石蜡切片组织钙交换体(NCX)蛋白表达NBT显色光学显微镜检测试剂盒10/20次

humaESTcorepressor3,RCOR3ELISA试剂盒人REST协阻抑因子3(RCOR3)ELISA试剂盒规格：96T/48T

Humaumorspecificaigen,TSAELISAKit人特异性抗原(TSA)ELISA试剂盒规格：96T/48T

区带蛋白PZP抗体规格 大鼠抑制因子(LIF)ELISA试剂盒 ，英文名： LIF ELISA Kit

Opioid receptor in mouse beta (beta -EPR) ELISA Kit 小鼠β内啡肽受体(β-EPR)ELISA试剂盒

ELISA 小鼠纤维蛋白原(mouse Fibrinogen) 48T/96T 进口分装

CLIAKitforIrna(HumanImmuneRNA)ELISAKit人免疫核糖核酸规格：48T/96T

通用型动物细胞周期(G1/S和G2/M期)同步(SYNCHRONY)处理试剂盒10次

ELISAKitTNF-β大鼠坏死因子β规格：48T/96T