产品订购信息：
英文名称  Anti-PARP (N-Terminus) 

中文名称   多腺苷二磷酸多聚酶抗体(N端)说明书 

别    名  ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP1; Poly (ADP ribose) polymerase 1; poly (ADP ribose) polymerase family, member 1; Poly adenosine diphosphate ADP ribose polymerase; Poly ADP ribose polymerase 1; Poly ADP ribose polymerase family member 1; Poly ADP ribose synthetase 1; poly(ADP ribose) synthetase; poly(ADP ribosyl)transferase; Poly[ADP ribose] synthetase 1; PPOL; sPARP 1; sPARP1; PARP1_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Cow 

产品类型   一抗  

研究领域    染色质和核信号 细胞凋亡

蛋白分子量  predicted molecular weight: 111kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PARP 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

多腺苷二磷酸多聚酶抗体(N端)说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008].

Function : Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Subunit : Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity). Interacts (when poly-ADP-ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.

Subcellular Location : Nucleus.

Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.

S-nitrosylated, leading to inhibit transcription regulation activity.

Similarity : Contains 1 BRCT domain.

Contains 1 PARP alpha-helical domain.

Contains 1 PARP catalytic domain.

Contains 2 PARP-type zinc fingers.

Database links : UniProtKB/Swiss-Prot: P09874.4

PARP(poly ADP-ribose polymerase)是DNA修复酶。

PARP是细胞凋亡核心成员半胱胺酸蛋白酶(caspase)的切割底物。因此,它在DNA损伤修复与细胞凋亡中发挥着重要作用。Anti-PARP p85 是特意的PARPp85片段的特异抗体，由caspase剪切116kDa完整分子而得到的。

PARP是存在于多数真核细胞中的一个多功能蛋白质翻译后修饰酶。它通过识别结构损伤的DNA片段而被激活，对聚腺苷二磷酸核糖聚合酶PARP被认为是DNA损伤的感受器。它还能对许多核蛋白进行聚腺苷二磷酸核糖基化。因此，在DNA损伤修复与细胞凋亡中发挥着重要作用，端锚聚合酶在癌细胞端粒结构的调控机制中有重要作用。

CA(Human Cholic acid) ELISA Kit 人胆酸Multi-class antibodies规格： 48T

Anti-Phospho-HSP27 (Ser15) 磷酸化热休克蛋白27抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh MARK4 丝氨酸/苏氨酸蛋白激酶MARK4抗体 规格 0.1ml

PPIL1(Human peptidylprolyl isomerase(cyclophilin)-like 1) ELISA Kit 人肽酰脯酰异构酶(亲环蛋白)样1 96T

RSBN1L 英文名称： 细胞碱性蛋白1样蛋白抗体 0.1ml

Cecropin 英文名称： 杀菌肽/天蚕抗菌肽单克隆抗体 1ml

Anti-Phospho-HSP27 (Ser15) 磷酸化热休克蛋白27抗体Multi-class antibodies规格： 0.1ml

M2-PK(Mouse Pyruvate Kinase) ELISA Kit 小鼠酸激酶M2型同工酶Multi-class antibodies规格： 48T

Anti-CD58/LFA-3/FITC 荧光素标记淋巴细胞功能相关抗原-3抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh DPY19L1 短粗矮胖19蛋白样1抗体 规格 0.2ml

RV IgM 胞病毒 IgM(捕获法二步法)

Midkine 英文名称： 中期因子/肝素结合生长因子抗体 0.1ml

Annexin A7 英文名称： 膜粘连蛋白7抗体 0.1ml

Anti-CD58/LFA-3/FITC 荧光素标记淋巴细胞功能相关抗原-3抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-Lyn (Tyr507) /FITC 荧光素标记膜相关蛋白酪氨酸激酶Lyn抗体IgGMulti-class antibodies规格： 0.2ml

Anti-phospho-Tau protein (Ser422)/FITC 荧光素标记磷酸化微管相关蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ADCK3/CABC1 伴侣蛋白bc1同源复合体抗体 规格 0.2ml

Rabbit Anti-dog IgG/FITC FITC标记的兔抗狗IgG 0.3ml

GBP5 英文名称： G蛋白结合蛋白5抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-chicken IgG/Cy7 Cy7标记的兔抗鸡IgG 规格 0.1ml

Anti-phospho-Tau protein (Ser422)/FITC 荧光素标记磷酸化微管相关蛋白抗体IgGMulti-class antibodies规格： 0.2ml

大鼠基质金属蛋白酶7(MMP-7)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人鞘脂激活蛋白原(PSAP)免疫试剂盒 human prosaposin,PSAP Elisa kit

Mousemaixmetalloproteinase2/GelatinaseA,MMP-2ELISAKit小鼠基质金属蛋白酶2/明胶酶A(MMP-2/GelatinaseA)ELISA试剂盒规格：96T/48T

P27蛋白表达西方杂交分析试剂盒5次

Humanoctameranscriptionfactor2B,OTF2BELISA试剂盒人八聚体转录因子(OTF2B)ELISA试剂盒规格：96T/48T

humaeceptoNFRSF-ieractingserine-threoninekinase2,RIPK2ELISAKit人受体TNFRSF结合丝氨酸苏氨酸激酶2(RIPK2)ELISA试剂盒规格：96T/48T

大鼠β淀粉样蛋白1-40(Aβ1-40)ELISA试剂盒 ，英文名： Aβ1-40 ELISA Kit

Mouse coicoopin releasing hormone (CRH) ELISA Kit 小鼠促肾上皮质激素释放激素(CRH)ELISA试剂盒

ELISA 小鼠抑瘤素mouse Oncostatin M(mouse OSM) 48T/96T 进口分装

CLIAKitforIL-1ELISAKit大鼠白介素1规格：48T/96T

通用型南瓜黄化花叶病毒(ZucchiniYellowMosaicVirus;ZYMV)试剂盒20次

ELISAKitaGT大鼠紧张素原规格：48T/96T

多腺苷二磷酸多聚酶抗体(N端)说明书 大鼠白介素8(IL-8/CXCL8)ELISA试剂盒 ，英文名： IL-8/CXCL8 ELISA Kit

Mouse ierleukin 13 (IL-13) ELISA Kit 小鼠白介素13(IL-13)ELISA试剂盒

ELISA 小鼠神经胶质细胞衍化营养因子(mouse GDNF) 48T/96T 进口分装

CLIAKitfori-PTHELISAKit大鼠全段甲状旁腺素规格：48T/96T

通用型非洲(Africanswinefevervirus;ACSF)试剂盒20次

ELISAKitLPL大鼠脂蛋白脂酶规格：48T/96T

抗体的生物素化标记实验要点：

1. 多腺苷二磷酸多聚酶抗体(N端)说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）多腺苷二磷酸多聚酶抗体(N端)说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。