抗体的生物素化标记实验要点：

1. P选择素糖蛋白配体1抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-PSGL-1/CD162 

中文名称   P选择素糖蛋白配体1抗体品牌 

别    名  CD 162; CD162; CD162 antigen; CLA; Cutaneous lymphocyte associated associated antigen; P selectin glycoprotein ligand 1; P selectin glycoprotein ligand 1 precursor; P-selectin glycoprotein ligand 1; PSGL 1; PSGL-1; PSGL1; Selectin P ligand; SELPL_HUMAN; SELPLG.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human 

产品类型   一抗  

研究领域    细胞生物 免疫学 细胞粘附分子 细胞表面分子

蛋白分子量  predicted molecular weight: 41kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PSGL-1 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

P选择素糖蛋白配体1抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011].

Function : A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Subunit : Homodimer; disulfide-linked. Interaction with P-, E- and L-selectins, through their lectin/EGF domains, is required for promoting recruitment and rolling of leukocytes. These interactions require sialyl Lewis X glycan modification but there is a differing dependence for tyrosine sulfations. Sulfation on Tyr-51 of PSGL1 is most important for high affinity L-selectin/SELL binding while P-selectin/SELP requires sulfation on Tyr-48. E-selectin/SELE binds with much lower affinity and requires the sLe(x) epitope, but apparantly not tyrosine sulfation. Dimerization appears not to be required for P-selectin/SELP binding. Interacts with SNX20. Interacts with MSN and SYK; mediates the activation of SYK by SELPLG.

Subcellular Location : Membrane; Single-pass type I membrane protein.

Post-translational modifications : Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.

Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.

DISEASE : Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.

Similarity : Belongs to the selectin/LECAM family.

Contains 1 C-type lectin domain.

Contains 1 EGF-like domain.

Contains 8 Sushi (CCP/SCR) domains.

Database links : UniProtKB/Swiss-Prot: Q14242.1

PSGL-1广泛分布于免疫细胞表面，在生物体内可与P、L、E选择素结合。在发生、发展中有重要作用.

抗体的鉴定：

1）P选择素糖蛋白配体1抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

NAP-2/CXCL7(Human neutrophil activating protein-2) ELISA Kit 人中性粒细胞趋化蛋白2Multi-class antibodies规格： 48T

Anti-CXCL4/PF-4 血小板因子4抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh MMP-9 基质金属蛋白酶-9抗体 规格 0.1ml

MMP-13(Human Matrix metalloproteinase 13) ELISA kit 人基质金属蛋白酶13 96T

RSL1D1 英文名称： 细胞衰老抑制基因蛋白抗体 0.2ml

phospho-Cdc6 (Ser54) 英文名称： 磷酸化细胞分裂周期蛋白6抗体 0.1ml

Anti-CXCL4/PF-4 血小板因子4抗体Multi-class antibodies规格： 0.2ml

PIBF(progesterone induced blocking factor) 孕激素诱导阻断因子(抗原)Multi-class antibodies规格： 0.5mg

Anti-CD57 CD57抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh P21/CDKN1A p21蛋白抗体 规格 0.1ml

TNFRSF19 Kit Mouse 小鼠 TNFRSF19 / TROY ELISA配对抗体 ELISA

TRIB1 英文名称： G蛋白偶联诱导蛋白2受体抗体 0.2ml

COLEC10(liver) 英文名称： 凝集蛋白家族10抗体(肝) 0.2ml

Anti-CD57 CD57抗体Multi-class antibodies规格： 0.1ml

Anti-HCV-NS4a/FITC 荧光素标记病毒-NS4a抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-Paxillin (Tyr118)/FITC 荧光素标记磷酸化桩蛋白Paxillin抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh BAG5 BCL2分子伴侣调节蛋白5抗体 规格 0.2ml

ALK (Anaplastic Lymphoma Kinase)(CD246Ag) 间变型激酶(抗原) 0.5mg

GBP2 英文名称： G蛋白结合蛋白2抗体 0.1ml

Rhesus antibody Rh Rabbit anti-Rat IgG whole serum 兔抗大鼠IgG抗血清 规格 1ml

Anti-Phospho-Paxillin (Tyr118)/FITC 荧光素标记磷酸化桩蛋白Paxillin抗体IgGMulti-class antibodies规格： 0.2ml

大鼠基质金属蛋白酶3(MMP-3)ELISA 试剂盒 96T/48T 试剂盒 组装/原装

人鞘氨醇(SPH)免疫试剂盒 Human Sphingosine,SPH Elisa Kit

MouseMethemoglobin,MHBELISAKit小鼠高铁血红蛋白(MHB)ELISA试剂盒规格：96T/48T

PAR-1蛋白表达西方杂交分析试剂盒5次

humanoncoproteininducedanscript3,OIT3ELISA试剂盒人癌蛋白诱导转录物3(OIT3)ELISA试剂盒规格：96T/48T

Humaecombinationactivatinggene1,RAG-1ELISAKit人重组激活基因1(RAG-1)ELISA试剂盒规格：96T/48T

大鼠β半乳糖苷酶(βGAL)ELISA试剂盒 ，英文名： βGAL ELISA Kit

Rabbit endothelial niic oxide syhase 3 (eNOS-3) ELISA Kit 兔子内皮型合成酶3(eNOS-3)ELISA试剂盒

ELISA 小鼠骨保护素配体(mouse OPGL) 48T/96T 进口分装

CLIAKitforIL-1Beta(HumanIerleukin1Beta)ELISAKit人白介素1β(IL-1B)规格：48T/96T

通用型牛(BovineLeukemiaVirus/EnzooticBovineLeukosis;BLV/EBL)试剂盒20次

ELISAKitAng-Ⅰ大鼠紧张素Ⅰ规格：48T/96T

P选择素糖蛋白配体1抗体品牌 大鼠白介素7受体(IL-7R)ELISA试剂盒 ，英文名： IL-7R ELISA Kit

Mouse ierleukin 16 (IL-16) ELISA Kit 小鼠白介素16(IL-16)ELISA试剂盒

ELISA 小鼠心肌转录因子3(mouse GATA3) 48T/96T 进口分装

CLIAKitforIP-10/CXCL10(Humanierferon-inducibleprotein10)ELISAKit人干扰素诱导蛋白10规格：48T/96T

通用型福氏志贺氏菌(Shigellaflexneri)试剂盒20次

ELISAKitLp-α大鼠脂蛋白α规格：48T/96T