抗体的生物素化标记实验要点：

1. 磷酸化蛋白酶体PSMα3抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-Proteasome 20S alpha 3(Ser250) 

中文名称   磷酸化蛋白酶体PSMα3抗体费用 

别    名  Proteasome 20S alpha 3 (phospho S250); 20S Proteasome α3; HC8; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome (prosome macropain) subunit alpha type 3; Proteasome alpha 3 subunit; Proteasome component C8; Proteasome subunit alpha type 3; Proteasome subunit alpha type-3; Proteasome subunit C8; PSA3_HUMAN; PSC3; PSMA3.

浓    度   1mg/1ml

规 格  0.1ml/100μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep 

产品类型   一抗 磷酸化抗体  

研究领域    细胞生物 细胞周期蛋白 细胞分化

蛋白分子量  predicted molecular weight: 28kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Proteasome 20S alpha 3 around the phosphorylation site of Ser250 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

磷酸化蛋白酶体PSMα3抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.

Function : The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.

Subunit : The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Interacts with AURKB. Interacts with CDKN1A. Interacts with -1 TAT protein. Interacts with hepatitis C virus (HCV) F protein. Interacts with Epstein-Barr virus EBNA3 proteins. Interacts with MDM2 and RB1. Interacts with the C-terminus of TBXA2R isoform 2.

Subcellular Location : Cytoplasm. Nucleus.

Database links : UniProtKB/Swiss-Prot: P25788.2

抗体的鉴定：

1）磷酸化蛋白酶体PSMα3抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

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PDGF-A 血小板源性生长因子-1(抗原) 0.5mg

hnRNP M 英文名称： 异质性核糖核蛋白M抗体 0.2ml

Rhesus antibody Rh RNF32 环指蛋白32抗体 规格 0.2ml

Anti-Phospho-NDRG1 (Thr346)/FITC 荧光素标记磷酸化分化相关基因NDRG1抗体IgGMulti-class antibodies规格： 0.2ml

Anti-COX10/FITC 荧光素标记COX10抗体IgGMulti-class antibodies规格： 0.2ml

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Donkey Anti-rabbit IgG/Alexa Fluor 350 Alexa Fluor 350标记的驴抗兔IgG 0.1ml

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phospho-PTPN7 (Ser93) 英文名称： 磷酸化非受体型蛋白酪酸磷酸酶7抗体 0.1ml

CD15 英文名称： 阶段特异性胚胎表面抗原-1抗体 0.1ml

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CM-H004人肺大内皮细胞完全培养基100mL

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原代软骨细胞特制基础培养基Many types of cells包装：500/250/100ml

DU145细胞，人细胞 人癌细胞,LCC1细胞 间充质干细胞培养基MSCM-acf-prf

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IL2RG Others Mouse 小鼠 IL2RG 人细胞裂解液 (阳性对照)

CM-H003人肺大动脉平滑肌细胞完全培养基100mL

NGFR Others Human 人 NGFR/ P75 人细胞裂解液 (阳性对照)

I型胶原酶(含10mL酶解缓冲液) 1mL

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IL21 Protein Mouse 重组小鼠 IL21 / Ierleukin 21 蛋白

人表皮黑色素细胞-深色素(HEM)( 5×105 ) MN-h, 小鼠海马神经元 Mouse

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SHG-44 (人细胞) 5×106cells/瓶×2

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