抗体的生物素化标记实验要点：

1. 蛋白酶体PSMα1抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-Proteasome 20S C2 

中文名称   蛋白酶体PSMα1抗体品牌 

别    名  20S Proteasome α1; 30 kDa prosomal protein; HC 2; HC2; Macropain subunit C2; Macropain subunit nu; Multicatalytic endopeptidase complex subunit C2; NU antibody PROS 30; PROS-30; PROS30; Proteasome (prosome macropain) subunit alpha type 1; Proteasome alpha 1 subunit; Proteasome component C2; Proteasome nu chain; Proteasome subunit alpha type 1; Proteasome subunit alpha type I; Proteasome subunit alpha type-1; Proteasome subunit nu; Protein P30 33K; PSA1_HUMAN; PSC 2; PSC2; PSMA 1; psmA1.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Cow, Horse 

产品类型   一抗  

研究领域    细胞生物 免疫学

蛋白分子量  predicted molecular weight: 30kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Proteasome 20S C2 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

蛋白酶体PSMα1抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Ubiquitin-dependent proteolysis mediates selective destruction of various cell cycle regulators, transcription factors and tumor suppressors. In eukaryotic cells, selective breakdown of cellular proteins is ensured by their ubiquitination and subsequent degradation by the 26S proteasome. At specific stages of development, embryo- and tissue-specific components of the 26S proteasome form, facilitating proteolysis. 20S Proteasome ?, also designated macropain subunit C2 or PROS-30, is a prosomal protein involved in a non-lysosomal ATP/ubiquitin-dependent proteolytic pathway. The entire proteasome is composed of at least 15 non-identical subunits which form a highly-ordered ring-shaped structure.

Function : The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.

Subunit : The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Interacts with bacterial

Subcellular Location : Cytoplasm. Nucleus.

Database links : UniProtKB/Swiss-Prot: P25786.1

抗体的鉴定：

1）蛋白酶体PSMα1抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-GDF8/MSTN/FITC 荧光素标记羊抗生长分化因子8/肌肉抑制素抗体IgGMulti-class antibodies规格： 0.2ml

Anti-NSE/FITC 荧光素标记神经元特异性烯醇化酶抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh BMPR1A/CD292 骨成型蛋白受体1A抗体 规格 0.1ml

GHRF (GHRH) 生长激素释放激素(因子)(抗原) 0.5mg

hnRNP U 英文名称： 异质核糖核蛋白U抗体 0.2ml

Rhesus antibody Rh RED/CSA2 软相关蛋白2抗体 规格 0.2ml

Anti-NSE/FITC 荧光素标记神经元特异性烯醇化酶抗体IgGMulti-class antibodies规格： 0.2ml

POMC(Human pro-opiomelanocortin) ELISA Kit 人阿黑皮素原Multi-class antibodies规格： 48T

Anti-PKB/AKT-1 蛋白激酶B(小鼠来源抗体)Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh HIP2 泛素蛋白连接酶E2抗体 规格 0.2ml

ANG- I R(Human angiotension I receptor Antibody) ELISA Kit 人紧张素Ⅰ受体抗体 96T

EV71 polyprotein VP1 英文名称： 肠道病毒71型/病毒抗体 0.2ml

CD34 英文名称： CD34抗体 0.1ml

Anti-PKB/AKT-1 蛋白激酶B(小鼠来源抗体)Multi-class antibodies规格： 0.1ml

RF(Human rheumatoid factor) ELISA Kit 人类因子Multi-class antibodies规格： 48T

Anti-Phospho-SEK1/MKK4 (Thr261) 磷酸化原活化蛋白激酶激酶4抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh HSPBAP1 热休克蛋白27相关蛋白1抗体 规格 0.2ml

T(Mouse Testoterone)ELISA Kit 小鼠睾酮 96T

phospho-PTPN6(Tyr564) 英文名称： 磷酸化蛋白酪酸磷酸酶1C抗体 0.1ml

CCDC148 英文名称： 卷曲螺旋结构域蛋白148抗体 0.2ml

Anti-Phospho-SEK1/MKK4 (Thr261) 磷酸化原活化蛋白激酶激酶4抗体Multi-class antibodies规格： 0.1ml

CL-0467WPMY-1(人正常基质永生化细胞)5×106cells/瓶×2

CXCL16 Others Rat 大鼠 CXCL16 / SR-PSOX 人细胞裂解液 (阳性对照)

大鼠虹膜色素上皮细胞完全培养基 100mL

U-373MG人 U-373MG glioma cells DMEM+10% FBS

IL1B Protein Rabbit 重组兔 IL-1 beta / IL1B 蛋白

NCI-H358(人非小细胞细胞) 5×106cells/瓶×2 PC-12(肾上腺嗜铬细胞瘤细胞)

CL-0464USMC(大鼠平滑肌细胞)5×106cells/瓶×2

CD44 Others Human 人 CD44 人细胞裂解液 (阳性对照)

人肝内胆管上皮细胞RNAHIBEpiC miRNA5 μg

5637(HTB-9)细胞，人细胞 大鼠细胞(癌细胞接种到*成瘤),Walker-256细胞 MLF, 小鼠淋巴成纤维细胞

HO-8910PM(人高转移细胞) 5×106cells/瓶×2

hMSC-AT-c 人类脂肪组织间质干细胞(hMSC-AT) 500,000cells 小鼠背脊髓神经元MN-dsc

蛋白酶体PSMα1抗体品牌 PROCR Others Cynomolgus 食蟹猴 Epcr / PROCR 人细胞裂解液 (阳性对照)

罗猴胎肾细胞;MA104

CL-0229T84(人腺癌肺转移细胞)5×106cells/瓶×2

SH-77细胞，小细胞 人T细胞细胞,T-CEM细胞 人T转基因细胞;Jurkat D,E

A-375(人恶性细胞) 5×106cells/瓶×2

BACE1 Others Human 人 BACE1 / ASP2 人细胞裂解液 (阳性对照)