抗体的生物素化标记实验要点：

1. 成对样同源结构域转录因子2抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-PITX2 

中文名称   成对样同源结构域转录因子2抗体说明书 

别    名  All1 responsive gene 1; ALL1 responsive protein ARP1; ALL1-responsive protein ARP1; ARP 1; ARP1; Brx 1; Brx1; Homeobox protein PITX2; IDG 2; IDG2; IGDS 2; IGDS; IGDS2; IHG 2; IHG2; IRID 2; IRID2; Otlx 2; Otlx2; Paired like homeodomain transcription factor 2; Paired-like homeodomain transcription factor 2; Pituitary homeobox 2; PITX 2; pitx2; PITX2_HUMAN; PTX 2; PTX2; RGS; RIEG 1; RIEG; Rieg bicoid related homeobox transcription factor 1; RIEG bicoid related homeobox transcription factor; RIEG bicoid-related homeobox transcription factor; RIEG1; RS antibody solurshin.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit 

产品类型   一抗  

研究领域    心 发育生物学 转录调节因子 表观遗传学

蛋白分子量  predicted molecular weight: 35kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PITX2 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

成对样同源结构域转录因子2抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Pitx2 also regulates lung symmetry by encoding “leftness” of the lung. Pitx2 is asymmetrically expressed in the left lateral-plate mesoderm, and mutant mice with laterality defects show altered patterns of Pitx2 expression that correlate with changes in the visceral symmetry. The genes which encode Pitx1 and Pitx2 map to human chromosomes 5q31 and 4q25-q26, respectively.May play an important role in development and maintenance of anterior structures. Isoform PTX2C is involved in left-right asymmetry the developing embryo.

Function : Controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, exerts a role in the expansion of muscle progenitors. May play a role in the proper localization of asymmetric organs such as the heart and stomach. Isoform PTX2C is involved in left-right asymmetry the developing embryo.

Subcellular Location : Nucleus.

Post-translational modifications : Phosphorylation at Thr-90 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1.

Defects in PITX2 are the cause of iridogoniodysgenesis type 2 (IRID2) [MIM:137600]; also known as iridogoniodysgenesis syndrome 2 (IGDS2). It is an autosomal dominant inherited disease.

Defects in PITX2 are a cause of Peters anomaly (PAN) [MIM:604229]. It is a congenital defect of the anterior chamber of the eye.

Defects in PITX2 are associated with ring dermoid of cornea (RDC) [MIM:180550]. RDC is an autosomal dominantly inherited syndrome characterized by bilateral annular limbal dermoids with corneal and conjunctival extension.

Similarity : Belongs to the paired homeobox family. Bicoid subfamily.

Contains 1 homeobox DNA-binding domain.

Involvement in disease;

Defects in PITX2 are the cause of Axenfeld-Rieger syndrome type 1 (RIEG1); also known as Rieger syndrome type 1. RIEG1 is an autosomal dominant defect characterized by hypodontia (partial anodontia), anal stenosis, hypertelorism, mental deficiency, agenesis of the facial bones, with malformation of the anterior chamber of the eye.

Defects in PITX2 are the cause of iridogoniodysgenesis type 2 (IRID2); also known as iridogoniodysgenesis syndrome 2 (IGDS2). It is an autosomal dominant inherited disease.

Defects in PITX2 are a cause of Peters anomaly (PAN). It is a congenital defect of the anterior chamber of the eye.

Defects in PITX2 are associated with ring dermoid of cornea (RDC). RDC is an autosomal dominantly inherited syndrome characterized by bilateral annular limbal dermoids with corneal and conjunctival extension.

抗体的鉴定：

1）成对样同源结构域转录因子2抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-Phospho-FHIT (Tyr114) /FITC 荧光素标记抗磷酸化脆性组氨酸三联体抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-NFKBp65 (Ser276)/FITC 荧光素标记磷酸化细胞核因子NF-κB p65抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh BubR1 有丝分裂检验点蛋白BubR1抗体 规格 0.2ml

AVPR2(arginine vasopressin receptor 2) 精酸加压素受体2抗原 0.5mg

HOOK1 英文名称： HOOK1蛋白抗体 0.2ml

Rhesus antibody Rh RNF126 环指蛋白126抗体 规格 0.2ml

Anti-Phospho-NFKBp65 (Ser276)/FITC 荧光素标记磷酸化细胞核因子NF-κB p65抗体IgGMulti-class antibodies规格： 0.2ml

BrdU(Human Bromodeoxyuridine) ELISA Kit 人溴脱氧核苷尿嘧啶Multi-class antibodies规格： 48T

Anti-PIWIL1 piwi样1蛋白抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh HIPK3 Fas相互作用蛋白激酶3抗体 规格 0.2ml

OVA sIgG(Human ovalbumin specific IgG) ELISA Kit 人卵清蛋白特异性IgG 96T

STK18 英文名称： 丝酸/苏酸蛋白激酶18 0.2ml

Connexin-37 英文名称： 间隙连接蛋白37抗体 0.1ml

Anti-PIWIL1 piwi样1蛋白抗体Multi-class antibodies规格： 0.1ml

PLAP(Human Placental alkaline pkosphatase) ELISA Kit 人胎盘碱性磷酸酶Multi-class antibodies规格： 48T

Anti-Phospho-NPM (Thr95) 磷酸化核仁磷酸蛋白抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh IGSF21 免疫球蛋白超家族成员21抗体 规格 0.2ml

AsAb ELISA Kit 大鼠抗抗体 96T

Phospho-PTEN(Ser385) 英文名称： 磷酸化抑制基因PTEN抗体 0.1ml

CKIP-1 英文名称： 酪蛋白激酶2相互作用蛋白1抗体 0.2ml

Anti-Phospho-NPM (Thr95) 磷酸化核仁磷酸蛋白抗体Multi-class antibodies规格： 0.1ml

CL-0456TM4(正常小鼠Sertoli细胞)5×106cells/瓶×2

SIGIRR Others Human 人 SIGIRR / TIR8 人细胞裂解液 (阳性对照)

人膀胱基质成纤维细胞RNAHBdSF miRNA5 μg

T24细胞，人膀胱移行细胞癌细胞 大鼠细胞(wistar 大鼠),CBRH7919细胞 RN-c, 大鼠皮质神经元

Ishikawa(人细胞) 5×106cells/瓶×2

hPC-PL-c 人类胎盘周细胞(hPC-PL) 500,000cells 小鼠巨噬细胞MMa

CL-0454TE-11(人细胞)5×106cells/瓶×2

HA Others H7N9 甲型 H7N9 (A/Shanghai/2/2013) 血凝素 (Hemagglutinin / HA) 人细胞裂解液 (阳性对照)

人膀胱平滑肌细胞RNAHBdSMC miRNA5 μg

兔眼Tenon's囊成纤维细胞;RYTF 细胞，Hep 3B2.1-7细胞 IR983F细胞，大鼠细胞

人男性正常细胞;Hs68

HA Others H5N1 甲型 H5N1 (A/Hong Kong/483/97) 血凝素 (Hemagglutinin / HA) 人细胞裂解液 (阳性对照)

成对样同源结构域转录因子2抗体说明书 CDH13 Others Rat 大鼠 CDH13 / Cadherin-13 / H Cadherin 人细胞裂解液 (阳性对照)

大鼠肾上腺嗜铬细胞瘤细胞;PC-12 [PC12]

CM-M123小鼠角膜成纤维细胞完全培养基100mL

TU212细胞，人细胞 人脐内皮(具有T24细胞特性),ECV-304细胞 仓鼠卵巢细胞;Lec1

C4-2(人细胞) 5×106cells/瓶×2

MET Others Human 人 c-MET / HGFR 人细胞裂解液 (阳性对照)