抗体的生物素化标记实验要点：

1. G蛋白偶联嘌呤受体p2y9抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-P2Y9 

中文名称   G蛋白偶联嘌呤受体p2y9抗体品牌 

别    名  5730485F04Rik; G protein coupled receptor 23; G-protein coupled receptor 23; GPR23; LPA receptor 4; LPA-4; LPA4; Lpar4; LPAR4_HUMAN; Lysophosphatidic acid receptor 4; Lysophosphatidic Acid Receptor P2Y9; P2RY9; P2Y purinoceptor 9; P2Y5 like; P2Y5-like receptor; P2Y9; Purinergic receptor 9.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep 

产品类型   一抗  

研究领域    神经生物学 通道蛋白 细胞膜受体 G蛋白偶联受体 G蛋白信号

蛋白分子量  predicted molecular weight: 42kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human P2Y9 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

G蛋白偶联嘌呤受体p2y9抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Nucleotides are emerging as important extracellular signaling molecules that mediate several effects, such as proliferation, differentiation , chemotaxis and cytokine release. The P2 receptor family is activated by the binding of nucleotides and is divided into two subfamilies, P2X and P2Y. The P2X receptor family is comprised of ligand-gated ion channels that allow for the increased permeability of calcium into the cell in response to extracellular ATP. The P2Y receptor family are G protein-coupled receptors which mediate the effects of extracellular nucleotides, primarily through the activation of phospholipase C. To some extent, the P2Y receptors can also activate potassium channels or, alternatively, inhibit adenylate cyclase and N-type calcium channels in response to extracellular nucleotides. P2Y9 is activated by lysophosphatidic acid (LPA), a lipid mediator involved in cell proliferation, differentiation, survival and death. In hamsters, P2Y9 mRNA is significantly expressed in ovary tissue compared to other tissues, and innervation with 1-oleoly LPA increases intracellular calcium ion concentration and stimulates adenylyl cyclase activity. P2Y9 is structurally related to nucleotide receptors, and shares 20-24% amino acid homology with the three other LPA receptors (LPA1, LPA2, LPA3).

Function : Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Transduces a signal by increasing the intracellular calcium ions and by stimulating adenylyl cyclase activity. The rank order of potency for agonists of this receptor is 1-oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1-alkenyl-LPA.

Subcellular Location : Cell membrane; Multi-pass membrane protein.

Tissue Specificity : High expression in ovary. Not detected in the brain regions thalamus, putamen, caudate, frontal cortex, pons, hypothalamus and hippocampus.

Database links : UniProtKB/Swiss-Prot: Q99677.1

抗体的鉴定：

1）G蛋白偶联嘌呤受体p2y9抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-Collagen VI /FITC 荧光素标记抗Ⅵ型胶原抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Kiss-1/FITC 荧光素标记转移抑制基因抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CCDC43 卷曲螺旋结构域蛋白43抗体 规格 0.2ml

HER2 receptor(NT)(erbB-2 isoform 2 receptor N-Terminus) c-erbB-2受体抗体(N端) 0.5mg

HOXA5 英文名称： 同源异型盒基因HOXA5蛋白抗体 0.1ml

Rhesus antibody Rh SMOC2 分泌模块化钙结合蛋白2抗体(平滑肌相关蛋白2) 规格 0.2ml

Anti-Kiss-1/FITC 荧光素标记转移抑制基因抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-Jak1 (Tyr1022/1023) /FITC 荧光素标记磷酸化蛋白质酪氨酸激酶JAK-1抗体IgGMulti-class antibodies规格： 0.2ml

Anti-PILR Beta/FDFACT/FITC 荧光素标记Ⅱ型成对免疫球蛋白样受体β蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh AMPD3 红细胞腺苷脱氨酶3抗体 规格 0.2ml

Rabbit Anti-mouse IgG-Fc/Alexa Fluor 555 Alexa Fluor 555标记的兔抗小鼠IgG-Fc 0.1ml

GNS 英文名称： 基葡萄糖6-酯酶抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-horse IgG/Cy5.5 Cy5.5标记的兔抗马IgG 规格 0.1ml

Anti-PILR Beta/FDFACT/FITC 荧光素标记Ⅱ型成对免疫球蛋白样受体β蛋白抗体IgGMulti-class antibodies规格： 0.2ml

LCN2(Human lipocalin 2) ELISA Kit 人脂质运载蛋白2Multi-class antibodies规格： 48T

Anti-Phospho-MAPKAPK2 (Thr334) 磷酸化原活化蛋白激酶活化的蛋白激酶2抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh KCNA7 电压阀门通道混合器相关亚家族成员7抗体 规格 0.1ml

PGE1 ELISA Kit 大鼠素E1 96T

phospho-PRL (Ser163) 英文名称： 磷酸化泌乳素抗体 0.1ml

Claudin 6 英文名称： 紧密连接蛋白6抗体 0.2ml

Anti-Phospho-MAPKAPK2 (Thr334) 磷酸化原活化蛋白激酶活化的蛋白激酶2抗体Multi-class antibodies规格： 0.1ml

CL-0443SK-N-MC(人神经上皮瘤细胞)5×106cells/瓶×2

NLGN1 Others Mouse 小鼠 Neuroligin 1 / NLGN1 人细胞裂解液 (阳性对照)

大鼠角膜内皮细胞完全培养基 100mL

MDBK (NBL-1)牛肾细胞 MDBK (NBL-1) of bovine kidney cell RPMI-1640(GIBCO)+10%FBS

IL18 Protein Mouse 重组小鼠 IL18 / IL-18 蛋白

NCI-H524(人非小细胞细胞) 5×106cells/瓶×2 MCF7(癌细胞)

CL-0440SK-MEL-1(人皮肤细胞)5×106cells/瓶×2

TNFSF11 Others Human 人 RANKL / OPGL / TNFSF11 / CD254 人细胞裂解液 (阳性对照)

人上皮细胞RNAHBEpiC miRNA5 μg

BIU-87细胞，人细胞 稳定转染了neor和LIF基因的STO细胞株,SNL细胞 CSC, 小鼠心肌细胞

M1(小鼠细胞) 5×106cells/瓶×2

NHEK adult single donor Pellet 正常人类表皮角质细胞团块(捐献者) > 1 mio.cells 小鼠肾小管上皮细胞MREpiC

G蛋白偶联嘌呤受体p2y9抗体品牌 ERBB4 Others Human 人 / 恒河猴 HER4 / ErbB4 杆状病毒-昆虫细胞裂解液 (阳性对照)

颌下腺上皮细胞Many types of cells包装：5 × 105次方(1ml)

人侵袭性脉络膜细胞;M619

叙利亚仓鼠肌肉细胞;GH-M1 猪髋动脉内皮细胞，PIEC细胞 CRL-1548细胞，大鼠细胞

人羊膜细胞;HA

CD68 Others Human 人 CD68 / Macrosialin / Gp110 人细胞裂解液 (阳性对照)