瘤促活化因子3抗体规格 英文名称  Anti-PEA3

中文名称  瘤促活化因子3抗体规格 

别    名 polymoma virus enhancer 3; ETV4; ets variant 4; ets; E1A-F; E1AF; PEA3; PEAS3.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Guinea Pig

产品类型   一抗  

研究领域     神经生物学 转录调节因子 表观遗传学

蛋白分子量  predicted molecular weight: 53kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human PEA3

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

瘤促活化因子3抗体规格 产品应用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Several members of the Ets gene family are known to encode sequence-specific DNA binding proteins. These include mouse PU.1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2 and rat GABP-a. Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element. For instance, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), but does not bind to the sequence 5'-AGGAAC-3', recognized by PU.1, although PU.1 binds equally well to both sequences. It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells. This is in contrast to other members of the Ets gene family, such as Ets-1, Ets-2 and Fli-1, each of which is expressed primarily in cells of hematopoietic origin.

Function : Pea3 is a member of the Ets gene family which are known to encode sequence specific DNA binding proteins. Pea3 binds the motif 5-AGGAAG-3 (the Pea-3 motif). It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. Pea3 is detected in cells of epithelial and fibroblastic origin. It is also an transcriptional activator that binds to the enhancer of the adenovirus E1A gene.

Subcellular Location : Nucleus.

Post-translational modifications : Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.

Contains 1 ETS DNA-binding domain.

PEA3属癌基因的转录因子Ets家族成员之一,多定位于细胞核。多瘤促活化因子(polymoma virus enhancer-3,PEA3)广泛表达于小鼠中,现有的研究已证实,PEA3与神经细胞的迁移、分化以及神经突触的形成过程有关.

实验流程：

全、新、优、品、好四大特点：

瘤促活化因子3抗体规格 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

正在热销的相关产品：

Anti-EAAT1/Glast /FITC 荧光素标记胶质细胞谷氨酸运载蛋白1 抗体IgGMulti-class antibodies规格： 0.2ml

Anti-MITF/FITC 荧光素标记小眼相关转录因子抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh C4orf27 4号染色体开放阅读框27抗体 规格 0.2ml

CD36 CD36(多肽) 0.5mg

HSP27 英文名称： 热休克蛋白27抗体 0.1ml

Rhesus antibody Rh SCF 干细胞生长因子抗体 规格 0.1ml

Anti-MITF/FITC 荧光素标记小眼相关转录因子抗体IgGMulti-class antibodies规格： 0.2ml

CH ELISA Kit 大鼠胆固醇Multi-class antibodies规格： 48T

Anti-Phospho-TAK1 (Ser412) 磷酸化转化生长因子β活化激酶1Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Goat Anti-Chicken IgG/PE-Cy7 PE-Cy7标记的羊抗鸡IgG 规格 0.1ml

5-HT(Mouse 5-Hydroxytryptamine) ELISA Kit 小鼠5羟色胺 96T

NTN3 英文名称： 轴突生长诱导因子3抗体 0.2ml

BMPER 英文名称： BMP内皮细胞调节蛋白抗体 0.2ml

Anti-Phospho-TAK1 (Ser412) 磷酸化转化生长因子β活化激酶1Multi-class antibodies规格： 0.1ml

ANG-1(Human Angiopoietin 1) ELISA Kit 人生成素1Multi-class antibodies规格： 48T

Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) 磷酸化p21激活激酶4、5、6抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Hpt/Haptoglobin 结合珠蛋白/触珠蛋白抗体 规格 0.1ml

ATGA/TGAB ELISA Kit 大鼠抗甲状腺球蛋白抗体 96T

Phospho-PDGFRA(Tyr849)/PDGFRB(Tyr857) 英文名称： 磷酸化血小板源性生长因子受体α/β抗体 0.1ml

CACNB4 英文名称： L型电压依赖型钙通道β4抗体 0.2ml

Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) 磷酸化p21激活激酶4、5、6抗体Multi-class antibodies规格： 0.1ml

CL-0291MKN-28(人高转移细胞)5×106cells/瓶×2

LTF Others Mouse 小鼠 Lactoansferrin / LTF 人细胞裂解液 (阳性对照)

小鼠*导管上皮细胞完全培养基 100mL

PC14人细胞 PC14 human lung cancer cells DMEM+10% FBS

IL36RN Protein Mouse 重组小鼠 IL1F5 / IL1RP3 蛋白

P3/NSI/1-Ag4-1 [NS-1] (小鼠细胞) 5×106cells/瓶×2 MC3T3-E1 Subclone 14(小鼠原成骨细胞)

CL-0288MA-891(小鼠癌高转移细胞)5×106cells/瓶×2

CLEC4F Others Rat 大鼠 CLEC4F / CLECSF13 人细胞裂解液 (阳性对照)

人前脂肪细胞-内脏cDNAHPA-v cDNA

BCaP-37细胞，人癌细胞 猴肾细胞系,MA-104细胞 肠内皮细胞Many types of cells包装：5 × 105次方(1ml)

TE-13(人细胞) 5×106cells/瓶×2

HBdMEC Pellet 人膀胱微内皮细胞团块 > 1 mio.cells 胰酶/EDTA消化液T/E

瘤促活化因子3抗体规格 FLT3 Others Mouse 小鼠 FLT-3 / CD135 / FLK-2 人细胞裂解液 (阳性对照)

细胞冻存液CFM

人胃腺癌细胞;BGC-823

CCC-HEL-1细胞，人胚肝二倍体细胞 小鼠腹水瘤细胞,SAC-II B2细胞 EB病毒转化的绒猴淋巴细胞;B95-8

MLTC-1(小鼠间质细胞瘤细胞) 5×106cells/瓶×2

Promocell C-23110 Fibroblast Growth Medium KIT,成纤维细胞生长培养基套装 500ml

技术外包服务:

★瘤促活化因子3抗体规格 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。