产品订购信息：
英文名称  Anti-FAK/PTK2 

中文名称   粘着斑激酶抗体品牌 

别    名  FADK 1; FADK; FAK 1; FAK related non kinase polypeptide; FAK1; Focal adhesion kinase 1; FRNK; pp125FAK; Protein tyrosine kinase 2; Protein Tyrosine Kinase Cytoplasmic; PTK 2; FAK1_HUMAN; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat 

产品类型   一抗  

研究领域     细胞生物 免疫学 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 116kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human FAK C-terminus 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

粘着斑激酶抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. [SUBCELLULAR LOCATION] Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Constituent of focal adhesions.

Function : Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

Subunit : Interacts (via first Pro-rich region) with CAS family members (via SH3 domain), including BCAR1, BCAR3, CASS4 and NEDD9. Interacts with GIT1. Interacts with SORBS1. Interacts with RGNEF. Interacts with SHB. Interacts with PXN and TLN1. Interacts with STAT1. Interacts with DCC. Interacts with WASL. Interacts with ARHGEF7. Interacts with GRB2 and GRB7 (By similarity). Component of a complex that contains at least FER, CTTN and PTK2/FAK1. Interacts with BMX. Interacts with TGFB1I1. Interacts with STEAP4. Interacts with ZFYVE21. Interacts with ESR1. Interacts with PIK3R1 or PIK3R2. Interacts with SRC, FGR, FLT4 and RET. Interacts with EPHA2 in resting cells; activation of EPHA2 recruits PTPN11, leading to dephosphorylation of PTK2/FAK1 and dissociation of the complex. Interacts with EPHA1 (kinase activity-dependent). Interacts with CD4; this interaction requires the presence of HIV-1 gp120. Interacts with PIAS1. Interacts with ARHGAP26 and SHC1. Interacts with RB1CC1; this inhibits PTK2/FAK1 activity and activation of downstream signaling pathways. Interacts with P53/TP53 and MDM2. Interacts with LPXN (via LD motif 3).

Subcellular Location : Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Nucleus. Note=Constituent of focal adhesions. Detected at microtubules.

Post-translational modifications : Phosphorylated on tyrosine residues upon activation, e.g. upon integrin signaling. Tyr-397 is the major autophosphorylation site, but other kinases can also phosphorylate this residue. Phosphorylation at Tyr-397 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-576, Tyr-577 and at additional tyrosine residues. FGR promotes phosphorylation at Tyr-397 and Tyr-576. FER promotes phosphorylation at Tyr-577, Tyr-861 and Tyr-925, even when cells are not adherent. Tyr-397, Tyr-576 and Ser-722 are phosphorylated only when cells are adherent. Phosphorylation at Tyr-397 is important for interaction with BMX, PIK3R1 and SHC1. Phosphorylation at Tyr-925 is important for interaction with GRB2. Dephosphorylated by PTPN11; PTPN11 is recruited to PTK2 via EPHA2 (tyrosine phosphorylated). Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly; this dephosphorylation could be catalyzed by PTPN11 and regulated by ZFYVE21.

Sumoylated; this enhances autophosphorylation.

DISEASE : Note=Aberrant PTK2/FAK1 expression may play a role in cancer cell proliferation, migration and invasion, in tumor formation and metastasis. PTK2/FAK1 overexpression is seen in many types of cancer.

Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family.

FAK subfamily.

Contains 1 FERM domain.

Database links : UniProtKB/Swiss-Prot: Q05397.2

FAK是整合蛋白介导的信号转导中的重要成员,有酪氨酸蛋白激酶活性,并可自身磷酸化,FAK本身是胱冬肽酶(caspase)的底物。作为信号分子的FAK参与的调节。

1.FAK 局部粘着斑激酶，是一种酪氨酸激酶;细胞的侵袭性生长是一个多步骤的复杂过程，有多种生物化学因子参与其中,局部粘着斑激酶（focal adhesion kinase, FAK）介导的信号转导系统就是其中最为重要的细胞信号转导途径之一。细胞必须黏附于细胞外基质，通过促进依赖于PTK激酶活性的细胞外基质信号转导，进而影响细胞的黏附、运动与迁移。

2.粘着斑激酶(focal adhesion kinase,FAK)是整合蛋白介导的信号转导中的重要成员,有酪氨酸蛋白激酶活性,并可自身磷酸化；为信号分子的FAK,还与细胞内其他信号转导通路存在串话(crosstalk),直接参与了细胞多种功能的调节。

3.尽管FAK的确切功能尚不清楚,但若干实验均提示FAK可能有两个作用,一是在细胞铺展和移动时,FAK参与粘着斑形成和调节；二是FAK参与信号转导过程,以告知细胞核其细胞已锚定了。近年有关FAK在细胞凋亡中的作用也业已肯定。

Anti-Gastrin/FITC 荧光素标记胃泌素/蛙皮素抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-NMDAR1 (Ser896) /FITC 荧光素标记磷酸化谷氨酸受体1抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Bone Sialoprotein/BSP 骨涎蛋白 规格 0.1ml

HSP-60( Heat shock protein-60) 热休克蛋白-60(抗原) 0.5mg

HSPABP/HIP/HSC70 Interacting Protein HIP 英文名称： 热休克蛋白70相互作用蛋白抗体 0.2ml

Rhesus antibody Rh Reprimo 细胞质内的糖基化蛋白抗体 规格 0.1ml

Anti-Phospho-NMDAR1 (Ser896) /FITC 荧光素标记磷酸化谷氨酸受体1抗体IgGMulti-class antibodies规格： 0.2ml

GHRP-Ghrelin(Human growth hormone releasing peptide-Ghrelin) ELISA Kit 人生长激素释放肽ghrelinMulti-class antibodies规格： 96T

Anti-Phospho-Stathmin (Ser16) 磷酸化原癌基因蛋白18Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh LAG-3/CD223 淋巴细胞活化基因-3抗体 规格 0.2ml

U S-GH(Human Ultra Sensitive growth hormone) ELISA Kit 人超敏生长激素 96T

PPP2R1B 英文名称： 蛋白质磷酸酶2调节亚基1B抗体 0.2ml

CHCHD1 英文名称： 卷曲螺旋结构域蛋白CHCHD1抗体 0.1ml

Anti-Phospho-Stathmin (Ser16) 磷酸化原癌基因蛋白18Multi-class antibodies规格： 0.1ml

AChE(Human Acetylcholinesterase) ELISA Kit 人乙酰胆碱酯酶Multi-class antibodies规格： 48T

Anti-NFBD1/MDC1 DNA损伤关卡蛋白1抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh IL-1RA 白介素-1受体拮抗剂抗体 规格 0.2ml

LIF ELISA Kit 大鼠抑制因子 96T

Phospho-Paxillin(Tyr88) 英文名称： 磷酸化桩蛋白Paxillin抗体 0.1ml

COX IV 英文名称： 细胞色素c氧化酶IV亚型1抗体 0.2ml

Anti-NFBD1/MDC1 DNA损伤关卡蛋白1抗体Multi-class antibodies规格： 0.2ml

CL-0261BC-022(人癌细胞)5×106cells/瓶×2

IFNB1 Others Mouse 小鼠 IFNB1 / IFN-beta / Ierferon beta 人细胞裂解液 (阳性对照)

人髓核细胞cDNAHNPC cDNA

T47D细胞，人管癌细胞 猪肾细胞系,IBRS-2细胞 Many types of cells包装：5 × 105次方(1ml)

MC3T3-E1(小鼠胚胎成骨细胞前体细胞) 5×106cells/瓶×2

HCMEC Pellet 人微内皮细胞团块 > 1 mio.cells 纤维母细胞粘附检测试剂盒Fibro

CL-0259BC-020(人癌细胞)5×106cells/瓶×2

FAM171B Others Human 人 FAM171B / KIAA1946 人细胞裂解液 (阳性对照)

人颅骨造骨细胞cDNAHCO cDNA

猪肾细胞;PK(15) 细胞，FO细胞 G422细胞，KM小鼠脑神经胶质母细胞瘤瘤株

表达SV40T和EBNA1的人胚肾细胞;293ET

HA Others H5N1 甲型 H5N1 (A/Egypt/2321-NAMRU3/2007) 血凝素 (Hemagglutinin / HA) 人细胞裂解液 (阳性对照)

粘着斑激酶抗体品牌 IL12A & IL12B Others Mouse 小鼠 IL12A & IL12B Heterodimer 人细胞裂解液 (阳性对照)

兔主动脉平滑肌细胞;SMC

J82 人膀胱移行细胞癌

NCI-H716 [H716]人结直肠腺癌细胞 NCI-H716 [H716] human colorectal adenocarcinoma cells RPMI-1640(GIBCO)+10%FBS

VEGFA Protein Human 重组人 / 食蟹猴 VEGF / VEGFA / VEGF165 蛋白

小鼠中脑缝神经细胞(脑脊)(MN-r)(1×106) HT-29, 人细胞 Human

抗体的生物素化标记实验要点：

1. 粘着斑激酶抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）粘着斑激酶抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。