抗体的生物素化标记实验要点：

1. P73抑制基因抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-P73 protein 

中文名称   P73抑制基因抗体规格 

别    名  p53 like transcription factor; p53 Related Protein; p73; TP 73; TP73; Tumor Protein p73; p53-like transcription factor; p53-related protein; P73_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat 

产品类型   一抗  

研究领域     细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子

蛋白分子量  predicted molecular weight: 70kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from mouse P73 protein (591-631aa) 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

P73抑制基因抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 P73 protein is a structural and functional homologue of p53, a tumor suppressor gene. In this study, The p73 protein, p19ras, by the yeast two-hybrid screening method. Alternative splicing of the proto-oncogene H-ras pre-mRNA has led to two distinct transcripts, Ras proteins are known to be small membrane-localized guanine nucleotide-binding proteins. However, unlike other Ras proteins, p19ras is localized in the nucleus and the cytosol and its interaction with P73 protein occurred exclusively in the nucleus. Oncogenic MDM2 (mouse double minutes 2) is a known repressor of p73 transcriptional activity. In this study, when p19ras was bound to MDM2, it further inhibited the association of MDM2 to the p73 protein. Therefore, this study presents a novel pathway of Ras signaling that occurs in the nucleus, involving p19ras and p73.

Function : Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. [COFACTOR] Binds 1 zinc ion per subunit

Subunit : Found in a complex with p53/TP53 and CABLES1. The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Interacts with HECW2. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9. Isoform Beta interacts with WWOX. Interacts (via SAM domain) with FBXO45 (via B30.2/SPRY domain). Interacts with YAP1 (phosphorylated form). Interacts with HCK (via SH3 domain); this inhibits TP73 activity and degradation.

Subcellular Location : Nucleus. Cytoplasm. Note=Accumulates in the nucleus in response to DNA damage.

Post-translational modifications : Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Phosphorylation by PLK1 and PLK3 inhibits the transcription regulator activity and pro-apoptotic function.

Higher levels of phosphorylation seen in the brain from patients with Huntington disease.

Polyubiquitinated by RCHY1/PIRH2; leading to its degradation by the proteasome.

Similarity : Belongs to the p53 family.

Contains 1 SAM (sterile alpha motif) domain.

Database links : UniProtKB/Swiss-Prot: O15350.1 (human)

UniProtKB/Swiss-Prot: Q9JJP2.1 (mouse)

P73是p53基因家族的第一个成员p53基因是一个经典的基因,与p53在结构和功能方面具有很高的相似性。

抗体的鉴定：

1）P73抑制基因抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

Anti-Fabp2 /FITC 荧光素标记脂肪酸结合蛋白2抗体IgGMulti-class antibodies规格： 0.2ml

Anti-NB3/CNTN6/FITC 荧光素标记抗神经细胞NB3特定蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh C17orf49 17号染色体开放阅读框49抗体 规格 0.2ml

PR (Progestogerone receptor) 孕激素受体(抗原) 0.5mg

HSPBAP1 英文名称： 热休克蛋白27相关蛋白1抗体 0.1ml

Rhesus antibody Rh Robo 轴突导向受体抗体 规格 0.1ml

Anti-NB3/CNTN6/FITC 荧光素标记抗神经细胞NB3特定蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Anti-HMGA2/FITC 荧光素标记高迁移率族蛋白A2抗体IgGMulti-class antibodies规格： 0.2ml

Anti-phospho-STAT6(pThr641)/FITC 荧光素标记抗磷酸化信号转导和转录激活因子6抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ATG1/ULK1 自噬相关蛋白1抗体 规格 0.2ml

名 价 格(RMB)

GK3 英文名称： 甘油激酶3抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-mouse IgG-Fc/PE-Cy3 PE-Cy3标记的兔抗小鼠IgG-Fc 规格 0.1ml

Anti-phospho-STAT6(pThr641)/FITC 荧光素标记抗磷酸化信号转导和转录激活因子6抗体IgGMulti-class antibodies规格： 0.2ml

5-LO/LOX(Human 5-lipoxygenase) ELISA Kit 人5脂加氧酶Multi-class antibodies规格： 48T

Anti-Nogo-A 轴索过度生长抑制因子-A抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh IL-1 Alpha 白介素1α/IL-1α抗体 规格 0.1ml

IL-17 ELISA KIT 大鼠白介素17 96T

Phospho-Paxillin(Ser83) 英文名称： 磷酸化桩蛋白Paxillin抗体 0.1ml

CXCR1 英文名称： 细胞表面趋化因子受体1抗体 0.1ml

Anti-Nogo-A 轴索过度生长抑制因子-A抗体Multi-class antibodies规格： 0.1ml

CL-0256AM(人腺样囊性癌细胞(高转移))5×106cells/瓶×2

SCN3B Others Human 人 SCN3B 人细胞裂解液 (阳性对照)

小鼠*上皮细胞完全培养基 100mL

B16小鼠细胞 B16 mouse melanoma cells RPMI-1640(GIBCO)+10%FBS

IL33 Protein Human 重组人 IL33 / Ierleukin-33 / NF-HEV 蛋白

PC-3M-2B4(人低转移细胞株) 5×106cells/瓶×2 CHO/dhFr-(中国仓鼠二氢叶酸还原酶缺陷细胞)

CL-0253A-427(人细胞)5×106cells/瓶×2

PGF Others Mouse 小鼠 PIGF / PLGF 人细胞裂解液 (阳性对照)

人膀胱平滑肌细胞cDNAHBdSMC cDNA

SK-BR-3细胞，人癌细胞 草鱼肾细胞系,GIK细胞 肺大平滑肌细胞Many types of cells包装：5 × 105次方(1ml)

WL1(大鼠肺细胞) 5×106cells/瓶×2

NHDF Pellet 正常人皮肤成纤维细胞团块(少年者) > 1 mio.cells 硝基苯磷酸(pNPP)磷酸酶检测试剂盒pNPP

P73抑制基因抗体规格 CLEC1B Others Human 人 CLEC1B / CLEC2 人细胞裂解液 (阳性对照)

大额牛皮肤成纤维样细胞;BOS-2

HS683 人脑细胞

SW 780 [SW-780, SW780]人膀胱移行细胞癌 SW 780 [SW-780, SW780] in human bladder ansitional cell carcinoma L-15培养基(GIBCO)+10%FBS

VEGFA Protein Danio rerio (zebrafish) 重组斑马鱼 VEGF / VEGFA / VEGF165 蛋白

大鼠淋巴纤维细胞(RLF)(5×105) BPH-1, 人细胞 Human