技术外包服务:

★色素上皮源性因子抗体说明书 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

实验流程：

色素上皮源性因子抗体说明书 英文名称  Anti-PEDF/SERPINF1

中文名称  色素上皮源性因子抗体说明书 

别    名 pigment epithelium-derived factor; Serpin-F1; Stromal cell-derived factor 3; SDF-3; Caspin; Alpha 2 antiplasmin; EPC 1; EPC1; PIG 35; PIG35; Pigment epithelium derived factor; Proliferation inducing protein 35; SERPIN F1; Serpin peptidase inhibitor clade F member 1; SERPINF 1; SERPINF1.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow

产品类型   一抗  

研究领域     细胞生物 免疫学 神经生物学 生长因子和激素

蛋白分子量  predicted molecular weight: 46kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human PEDF C-terminus

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

色素上皮源性因子抗体说明书 产品应用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Pigment epithelium derived factor, originally identified in conditioned medium of cultured human fetal retinal pigment epithelial (RPE) cells, is a neurotrophic protein that induces extensive neuronal differentiation in human Y79 retinoblastoma cells, a neoplastic counterpart of normal retinoblasts. It has been suggested that PEDF is synthesized by RPE cells and secreted into the retina interphotoreceptor matrix where it may influence development/differentiation of the neural retina. PEDF is a potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The PEDF gene is a member of the serpin gene family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.

Function : Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Subcellular Location : Secreted. Melanosome. Enriched in stage I melanosomes.

Tissue Specificity : Retinal pigment epithelial cells and blood plasma.

N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

DISEASE : Defects in SERPINF1 are the cause of osteogenesis imperfecta type 12 (OI12) [MIM:613982]. OI12 is a connective tissue disorder characterized by bone fragility, low bone mass, and recurrent fractures. OI12 is characterized by features compatible with osteogenesis imperfecta type III in the Sillence classification. Patients have normal grayish sclerae and fractures of long bones and severe vertebral compression fractures, with resulting deformities observed as early as the first year of life.

Similarity : Belongs to the serpin family.

色素上皮衍生因子（PEDF）是人及动物眼中天然存在的一种糖蛋白，属于丝氨酸蛋白酶抑制剂家族，目前研究认为：它具有新生和神经营养功能的双重作用。

过去曾对PEDF的神经保护作用及研究较多，在很多组织中都有不同的表达。

目前研究认为；PEDF是最为有效的眼内新生天然抑制剂，对新生的形成起着重要的调控作用。

全、新、优、品、好四大特点：

色素上皮源性因子抗体说明书 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

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