抗体的生物素化标记实验要点：

1. 凋亡相关蛋白4抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-PDCD4 

中文名称   凋亡相关蛋白4抗体品牌 

别    名  PDCD-4; PDCD 4; Death up-regulated gene protein; Dug; H731; Ma3; MGC33046; MGC33047; Neoplastic transformation inhibitor; Neoplastic transformation inhibitor protein; Nuclear antigen H731; Nuclear antigen H731 like; Nuclear antigen H731 like protein; PDCD 4; Programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a; Protein MA-3; RP11 348N5.4; Tis; Nuclear antigen H731-like; Pdcd4; PDCD4_HUMAN; Topoisomerase-inhibitor suppressed protein; Topoisomerase-inhibitor suppressed protein (programmed cell death 4).

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat 

产品类型   一抗  

研究领域     细胞生物 染色质和核信号 细胞凋亡 表观遗传学

蛋白分子量  predicted molecular weight: 52kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PDCD4 C-terminus 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

凋亡相关蛋白4抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. Alternative names: H731; MGC33046; MGC33047; Neoplastic transformation inhibitor; Neoplastic transformation inhibitor protein; Nuclear antigen H731; Nuclear antigen H731 like; Nuclear antigen H731 like protein; OTTHUMP00000020483; PDCD 4; Programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor) ; Programmed cell death protein 4; Protein 197/15a ; RP11 348N5.4 .

Function : Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity).

Subunit : Interacts (via MI domains) with EIF4A2 (By similarity). Interacts (via MI domains) with EIF4A1 (via N-terminal domain). Heterotrimer with EIF4A1; one molecule of PDCD4 binds two molecules of EIF4A1. Interacts with EIF4G1. May form a complex with EIF4A1 and EIF4G1. The interaction between PDCD4 and EIF4A1 interferes with the interaction between EIF4A1 and EIF4G. When phosphorylated, interacts with BTRC and FBXW11.

Subcellular Location : Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.

Post-translational modifications : Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.

Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens; phosphorylation promotes proteasomal degradation of PDCD4.

Similarity : Belongs to the PDCD4 family.

Contains 2 MI domains.

Database links : UniProtKB/Swiss-Prot: Q53EL6.2

程序性细胞死亡因子4是近期发现一种新的基因，在很多组织中有不同的表达。

抗体的鉴定：

1）凋亡相关蛋白4抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

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Anti-GABA-AR Alpha1/FITC 荧光素标记γ1氨基受体α1抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CNDP1 肌肽二肽酶1抗体 规格 0.1ml

NR2A (Glutamate receptor2A) 谷酸受体(N端抗原) 0.5mg

IDH3G 英文名称： 异柠檬酸脱氢酶gamma亚基抗体 0.2ml

Rhesus antibody Rh TSARG3/TSARG1 生精细胞凋亡相关基因3抗体 规格 0.1ml

Anti-GABA-AR Alpha1/FITC 荧光素标记γ1氨基受体α1抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Jun D/FITC 荧光素标记活化蛋白激酶D抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-SHIP2 (Ser576) /FITC 荧光素标记磷酸化蛋白酪氨酸磷酸酶2抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh AMBRA1 自噬相关基因AMBRA1抗体 规格 0.2ml

Rabbit Anti-human sIgA/Alexa Fluor 488 Alexa Fluor 488标记的兔抗人分泌型IgA 0.1ml

GNB5 英文名称： G蛋白β5/Gβ 5 抗体 0.2ml

Rhesus antibody Rh Rabbit Anti-horse IgG/Alexa Fluor 350 Alexa Fluor 350标记的兔抗马IgG 规格 0.1ml

Anti-Phospho-SHIP2 (Ser576) /FITC 荧光素标记磷酸化蛋白酪氨酸磷酸酶2抗体IgGMulti-class antibodies规格： 0.2ml

人高香草酸(HVA) ELISA Kit 人高香草酸Multi-class antibodies规格： 48T

Anti-PI3K p85 (Tyr458)/p55 (Tyr199) 磷酸化磷脂酰肌醇激酶抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Histone H2A.x 组蛋白H2AX抗体 规格 0.2ml

SCA(Human steroid producing cell autoantibody) ELISA Kit 人抗类固醇生成细胞抗体 96T

phospho-p23 (Ser113) 英文名称： 磷酸化端粒酶结合蛋白P23抗体 0.1ml

Crkl 英文名称： 接头蛋白CrkL抗体 0.2ml

Anti-PI3K p85 (Tyr458)/p55 (Tyr199) 磷酸化磷脂酰肌醇激酶抗体Multi-class antibodies规格： 0.1ml

CL-0151MDA-MB-435S(人导管癌细胞)5×106cells/瓶×2

CD3E Others Cynomolgus 食蟹猴 CD3e / CD3 epsilon 人细胞裂解液 (阳性对照)

小鼠肝动脉内皮细胞完全培养基 100mL

HCAEC人内皮细胞 HCAEC in human coronary artery endothelial cells ECM(Sicencell Cat#1001)

IL11 Protein Human 重组人 IL11 / Ierleukin 11 / IL-11 蛋白

RKO-E6(人转基因细胞) 5×106cells/瓶×2 Jecket(细胞)

CL-0148MADB106(大鼠癌细胞)5×106cells/瓶×2

SELL Others Cynomolgus 食蟹猴 CD62L / L-Selectin / SELL 人细胞裂解液 (阳性对照)

人上皮细胞裂解物HPEpiCL

1590细胞，人癌细胞系 小鼠,EL-4细胞 脑平滑肌细胞Many types of cells包装：5 × 105次方(1ml)

Beta-TC-6(小鼠瘤胰岛β细胞) 5×106cells/瓶×2

HCASMC Pellet 人平滑肌细胞团块 > 1 mio.cells 人成纤维细胞-心室裂解物HCF-av L

凋亡相关蛋白4抗体品牌 TGM3 Others Human 人 TGM3 / ansglutaminase 3 杆状病毒-昆虫细胞裂解液 (阳性对照)

动物上皮细胞培养基EpiCM-a

人;HFL1

CRFK细胞，猫肾细胞 结细胞,LOVE1细胞 CM-H069人膀胱平滑肌细胞完全培养基100mL

大鼠胰岛β细胞瘤细胞;RIN-m5F

RSV-G Others Respiratory syncytial virus/RSV 人类合胞病毒 hRSV (A, rsb1734) glycoprotein G / RSV-G 人细胞裂解液 (阳性对照)