产品订购信息：
英文名称  Anti-Phospho-PRK1 (Thr774)/PRK2 (Thr816) 

中文名称   磷酸化腺衍生内皮生长因子抗体品牌 

别    名  Prokineticin-1/Endocrine-gland-derived vascular endothelial growth factor; PROK1; EGVEGF; PK1; EG-VEGF; PRK1.

浓    度   1mg/1ml

规 格  0.1ml/100μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit 

产品类型   一抗 磷酸化抗体  

研究领域     免疫学 内皮细胞

蛋白分子量  predicted molecular weight: 103kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthesised phosphopeptide derived from human PRK1 around the phosphorylation site of Thr774 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化腺衍生内皮生长因子抗体品牌 产品应用    ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Endocrine gland-derived vascular endothelial growth factor (EG-VEGF) induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. EG-VEGF possesses an HIF-1 binding site; its expression is induced by hypoxia and restricted to the steroidogenic glands (ovary, testis, adrenal and placenta). Expression of EG-VEGF is often complementary to the expression of VEGF, suggesting that these molecules function in a coordinated manner. EG-VEGF is an example of a class of highly specific mitogens that act to regulate proliferation and differentiation of the vascular endothelium in a tissue-specific manner. It is expressed primarily in one type of tissue and acts selectively on one type of endothelium. EG-VEGF, possibly through binding to a G protein-coupled receptor, results in the activation of MAPK p44/42 and phosphatidylinositol 3-kinase signaling pathways, leading to proliferation, migration and survival of responsive endothelial cells .

Function : Endocrine gland-derived vascular endothelial growth factor (EG-VEGF) induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. Its expression is induced by hypoxia and is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta). Its expression is often complementary to the expression of VEGF (MIM192240), suggesting that these molecules function in a coordinated manner.[supplied by OMIM].

Subcellular Location : Cytoplasm. Nucleus. Endosome. Cell membrane; Peripheral membrane protein.

Tissue Specificity : Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.

Anti-CD272/BTLA/FITC 荧光素标记B和T淋巴细胞衰减蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Anti-IL-6/FITC (mouse、rat) 荧光素标记白介素6抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CD24 CD24抗体 规格 0.1ml

MMP-17(Matrix metalloproteinase-17) 基质金属蛋白酶-17抗原 0.5mg

IFI44L 英文名称： 干扰素诱导蛋白44样蛋白抗体 0.2ml

Rhesus antibody Rh STK25/YSK1 丝氨酸/苏氨酸激酶25抗体 规格 0.2ml

Anti-IL-6/FITC (mouse、rat) 荧光素标记白介素6抗体IgGMulti-class antibodies规格： 0.2ml

AQP-0(Human Aquaporin 0) ELISA Kit 人水通道蛋白0Multi-class antibodies规格： 48T

Anti-Phospho-SEK1/MKK4 (Ser257) 磷酸化原活化蛋白激酶激酶4抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh HSPC117/C22orf28 22号染色体开放阅读框28抗体 规格 0.2ml

PL12/AlaRS(Human anti-PL12-antibody) ELISA Kit 人PL12抗体/抗丙酰tRNA合成酶 96T

PPP4C 英文名称： 丝酸/苏酸蛋酸酶4C抗体 0.2ml

CCDC153 英文名称： 卷曲螺旋结构域蛋白153抗体 0.2ml

Anti-Phospho-SEK1/MKK4 (Ser257) 磷酸化原活化蛋白激酶激酶4抗体Multi-class antibodies规格： 0.1ml

Alpha-GST ELISA Kit 大鼠α谷胱甘肽S转移酶Multi-class antibodies规格： 48T

Anti-TRIM32 TRIM32抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh GIMAP5 GTP酶IMAP家族成员5抗体 规格 0.2ml

CA-2(Mouse carbonic anhydrase 2) ELISA KIT 小鼠酶2 96T

Phospho-NMDAR2A (Tyr1325) 英文名称： 磷酸化谷酸受体2A抗体 0.1ml

ALS2CR8 英文名称： 肌侧索硬化2染色体区域候选蛋白8抗体 0.2ml

Anti-TRIM32 TRIM32抗体Multi-class antibodies规格： 0.1ml

CL-0103Hep G2(人细胞)5×106cells/瓶×2

IgG2a Others Mouse 小鼠 IgG2a-Fc 人细胞裂解液 (阳性对照)

小鼠肝窦内皮细胞完全培养基 100mL

RAG小鼠肾腺癌细胞 RAG mice renal adenocarcinoma cells MEM培养基(GIBCO)+10%FBS

LIF Protein Mouse 重组小鼠 LIF 蛋白 (Fc 标签)

RTE(大鼠气管上皮细胞) 5×106cells/瓶×2 A375(人类恶性)

CL-0100HEC-1-B(人子宫内膜腺癌细胞)5×106cells/瓶×2

CSF2RB Others Rat 大鼠 CD131 / CSF2RB / IL3RB / IL5RB 人细胞裂解液 (阳性对照)

人海马趾星形胶质细胞裂解物HA-h L

OVCaR-3细胞，人细胞 菜青虫卵巢细胞,Pr-HNV8细胞 神经胶质细胞Many types of cells包装：5 × 105次方(1ml)

CEM/C1(人急性淋巴细胞细胞) 5×106cells/瓶×2

HUtSMC Pellet 人细胞团块 > 1 mio.cells 人表皮角质细胞-cDNAHEK-acDNA

磷酸化腺衍生内皮生长因子抗体品牌 IGFBP6 Others Human 人 IGFBP6 / IBP-6 人细胞裂解液 (阳性对照)

人口腔角质细胞裂解物HOKL

BRL-3A 大鼠正常肝细胞

CL-0118HT-29(人细胞)5×106cells/瓶×2

人绒毛间充质成纤维细胞

人肝永生化细胞;THLE-3 人微内皮细胞完全培养基 100mL

抗体的生物素化标记实验要点：

1. 磷酸化腺衍生内皮生长因子抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）磷酸化腺衍生内皮生长因子抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。