产品订购信息：
英文名称  Anti-PSD93 

中文名称   突触后密度蛋白93抗体费用 

别    名  Channel associated protein of synapse 110; Channel associated protein of synapses 110kD; Channel-associated protein of synapse-110; Chapsyn 110; Chapsyn-110; Chapsyn110; discs large homolog 2; Discs, large homolog 2 (Drosophila); Disks large homolog 2; DKFZp781D1854; DKFZp781E0954; Dlg 2; dlg2; DLG2_HUMAN; Dlgh 2; Dlgh2; FLJ37266; Gm1197; MGC131811; Postsynaptic density protein PSD 93; Postsynaptic density protein PSD-93; Postsynaptic density protein PSD93; PSD 93; PSD93.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit 

产品类型   一抗  

研究领域    细胞生物 免疫学 神经生物学 信号转导 细胞类型标志物

蛋白分子量  predicted molecular weight: 98kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PSD93 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

突触后密度蛋白93抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 PSD 93 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD 93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with PSD 93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD 93 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Function : Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses (By similarity).

Subunit : Interacts through its PDZ domains with NETO1. Interacts with NOS1/nNOS through second PDZ domain (By similarity). Interacts with KCNJ2/Kir2.1 (via C-terminus) through one of its PDZ domains. Interacts with FRMPD4 (via C-terminus). Interacts with LRFN1, LRFN2 and LRFN4. Interacts with FASLG.

Subcellular Location : Membrane; Lipid-anchor (By similarity). Cell junction, synapse, postsynaptic cell membrane, postsynaptic density (By similarity). Cell junction, synapse (By similarity). Note=Concentrated in soma and postsynaptic density of a subset of neurons (By similarity).

Similarity : Belongs to the MAGUK family.

Contains 1 guanylate kinase-like domain.

Contains 3 PDZ (DHR) domains.

Contains 1 SH3 domain.

Database links : UniProtKB/Swiss-Prot: Q15700.3

Anti-CD146(MCAN)/FITC 荧光素标记抗细胞粘付分子CD146抗体IgGMulti-class antibodies规格： 0.2ml

Anti-CD122/IL-2RB/FITC 荧光素标记CD122/白介素2受体β链抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CD336/NKp44/NCR2 细胞毒性受体NK-p44抗体 规格 0.2ml

Nephrin Protein 去氧 0.5mg

IFI78 英文名称： 干扰素诱导蛋白P78抗体 0.1ml

Rhesus antibody Rh SULT2A1 胆盐磺基转移酶 规格 0.1ml

Anti-CD122/IL-2RB/FITC 荧光素标记CD122/白介素2受体β链抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-IRAK1 (Thr209) /FITC 荧光素标记磷酸化白介素-1受体相关激酶1抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Phospho-SATB1 (Ser47)/FITC 荧光素标记磷酸化核基质结合区结合蛋白1抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ANKRD12 锚蛋白重复结构域蛋白12抗体 规格 0.2ml

Rabbit Anti-MK IgM/PE PE标记的兔抗猴IgM 0.1ml

GABPB2 英文名称： 核呼吸因子2抗体 0.1ml

Rhesus antibody Rh Rabbit Anti-human IgG F(ab')2/Alexa Fluor 350 Alexa Fluor 350标记的兔抗人IgG F(ab')2 规格 0.1ml

Anti-Phospho-SATB1 (Ser47)/FITC 荧光素标记磷酸化核基质结合区结合蛋白1抗体IgGMulti-class antibodies规格： 0.2ml

AIF ELISA Kit 大鼠凋亡诱导因子Multi-class antibodies规格： 48T

Anti-TEM8 内皮标志物8抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Goat Anti-Bovine IgG/Cy5.5 Cy5.5标记的羊抗IgG 规格 0.1ml

ALP-B(Mouse Bone-specific Alkphase B) ELISA Kit 小鼠骨特异性碱性磷酸酶B 96T

Phospho-NMDAR1(Ser896) 英文名称： 磷酸化谷酸受体1抗体 0.1ml

Beta-lactoglobulin 英文名称： β-乳球蛋白抗体 1ml

Anti-TEM8 内皮标志物8抗体Multi-class antibodies规格： 0.2ml

CL-0098HCT-8(人盲肠腺癌细胞)5×106cells/瓶×2

RSPO1 Others Human 人 R-Spondin 1 / RSPO1 CHO细胞裂解液 (阳性对照)

人小脑星形胶质细胞裂解物HA-c L

新生牛眼Tenon's囊成纤维细胞;NBTF 腺癌细胞，RKO细胞 TA2细胞，小鼠自发高癌细胞

人原胚肾转化细胞;293 Ad5

TIMP1 Others Mouse 小鼠 TIMP1 / TIMP / CLGI 人细胞裂解液 (阳性对照)

CL-0096HCT 116(人细胞)5×106cells/瓶×2

SPG21 Others Human 人 SPG21 杆状病毒-昆虫细胞裂解液 (阳性对照)

人神经束膜细胞裂解物HPCL

TT细胞，人骨髓样细胞 人肾小球系膜细胞,HMC细胞 神经小胶质细胞Many types of cells包装：5 × 105次方(1ml)

兔肾细胞;RK13

SLIK1 Others Human 人 SLIK1 人细胞裂解液 (阳性对照)

突触后密度蛋白93抗体费用 CN2 Others Mouse 小鼠 Coactin 2 / CN2 人细胞裂解液 (阳性对照)

脑动脉平滑肌细胞Many types of cells包装：5 × 105次方(1ml)

大鼠主动脉内皮细胞完全培养基 100mL

PC-12(高分化)大鼠肾上腺嗜铬细胞瘤细胞(高分化) PC-12 (high differeiation) rat pheochromocytoma cells (high differeiation) RPMI-1640(GIBCO)+10%FBS

IFNA8 Protein Human 重组人 Ierferon alpha-B / IFNA8 蛋白 (His 标签)

HHCC(人细胞) 5×106cells/瓶×2 粘质沙雷伯氏菌

抗体的生物素化标记实验要点：

1. 突触后密度蛋白93抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）突触后密度蛋白93抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。