产品订购信息：
英文名称  Anti-PIM1 

中文名称   丝氨酸/苏氨酸激酶蛋白Pim1抗体规格 

别    名  Oncogene PIM 1; Oncogene PIM1; PIM 1; pim 1 kinase 44 kDa isoform; Pim 1 kinase; pim 1 oncogene (proviral integration site 1); Pim 1 oncogene; PIM; PIM1; pim1 kinase 44 kDa isoform; PIM1_HUMAN; Pim2; PIM3; Proto oncogene serine/threonine protein kinase Pim 1; Proto-oncogene serine/threonine-protein kinase Pim-1; Proviral integration site 1; Proviral integration site 2.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig 

产品类型   一抗  

研究领域     免疫学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 45kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PIM1 (257-301aa) 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

丝氨酸/苏氨酸激酶蛋白Pim1抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 PIM1 is a member of the serine/threonine kinase PIM oncogene family. PIM1 has been implicated in lymphomagenesis, cell proliferation, apoptosis, differentiation and tumourigenesis. The PIM1 protein kinase is upregulated in prostate cancer.

Function : Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerced by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B,induces 14-3-3-proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis.

Subunit : Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Binds to RP9. Isoform 1, but not isoform 2, binds BMX. Isoform 2 interacts with CDKN1B and FOXO3. Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM1, ubiquitination and proteasomal degradation. Interacts with HSP90, this interaction stabilizes PIM1 protein levels. Interacts (ubiquitinated form) with HSP70 and promotes its proteosomal degradation. Interacts with CDKN1A. Interacts with CDC25C. Interacts (via N-terminal 96 residues) with CDC25A. Interacts with MAP3K5. Interacts with MYC.

Subcellular Location : Isoform 2: Cytoplasm. Nucleus. Isoform 1: Cell membrane.

Post-translational modifications : Autophosphorylated on both serine/threonine and tyrosine residues. Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.

Ubiquitinated, leading to proteasomal degradation.

Similarity : Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.

Contains 1 protein kinase domain.

Database links : UniProtKB/Swiss-Prot: P11309.3

PIM1蛋白是丝氨酸/苏氨酸蛋白激酶2家族成员，参与细胞信号转导、细胞凋亡、转录调节，在很多中表达，尤其是，表达水平较高。

Anti-CD28/FITC 荧光素标记CD28蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Anti-HRG- Alpha/FITC 荧光素标记抗HRGα抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CD99/E2 antigen CD99抗体 规格 0.2ml

Podoplanin Protein 平足蛋白抗原 0.5mg

IFIT3 英文名称： 干扰素诱导的三角形四肽重复蛋白3抗体 0.2ml

Rhesus antibody Rh TCF12/HEB 转录因子12抗体 规格 0.2ml

Anti-HRG- Alpha/FITC 荧光素标记抗HRGα抗体IgGMulti-class antibodies规格： 0.2ml

Human anti-thrombin receptor,ATR ELISA Kit 人抗凝血酶受体Multi-class antibodies规格： 48T

Anti-Phospho-S6 Ribosomal Protein (Ser235/236) 磷酸化S6核糖体蛋白抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh GSK-3 Beta (CT) 葡萄糖合成激酶-3β抗体 规格 0.1ml

LHRH(Mouse Luteinizing Hormone-Releasing Hormone) ELISA Kit 小鼠黄体生成素释放激素 96T

OAS1 英文名称： 寡腺苷酸合成酶-1 0.1ml

Bim 英文名称： 细胞死亡调解子抗体 0.1ml

Anti-Phospho-S6 Ribosomal Protein (Ser235/236) 磷酸化S6核糖体蛋白抗体Multi-class antibodies规格： 0.1ml

Human Agrin ELISA Kit 人聚集蛋白Multi-class antibodies规格： 48T

Anti-SOD2 超氧化物歧化酶2抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Goat anti-Mouse IgM whole serum 羊抗小鼠IgM抗血清 规格 1ml

C4a ELISA Kit 大鼠过敏毒素/补体片断4a 96T

phospho-NIFK (Thr234) 英文名称： 磷酸化NIFK抗体 0.1ml

BMP11 英文名称： 骨形态发生蛋白11抗体 0.1ml

Anti-SOD2 超氧化物歧化酶2抗体Multi-class antibodies规格： 0.1ml

CL-0088H4-IIE(大鼠细胞 )5×106cells/瓶×2

VEGFA Others Human 人 VEGF / VEGFA / VEGF165 杆状病毒-昆虫细胞裂解液 (阳性对照)

人脉络丛成纤维细胞裂解物HCPFL

ES-2细胞，人卵巢透明细胞癌细胞 人肾上腺皮质腺癌细胞,NCI-295R细胞 肾成纤维细胞Many types of cells包装：5 × 105次方(1ml)

家兔皮肤细胞;DR-S1

SLIK1 Others Human 人 SLIK1 人细胞裂解液 (阳性对照)

CL-0087H4(人)5×106cells/瓶×2

IgG2 Others Human 人 IgG2-Fc (257 Ser/Ala) 人细胞裂解液 (阳性对照)

小鼠直肠平滑肌细胞完全培养基 100mL

SV40 MES 13小鼠肾小球系膜细胞 SV40 MES 13 in mouse glomerular mesangial cells DMEM培养基+5%FBS

CF Protein Rat 重组大鼠 CF / Ciliary Neuroophic Factor 蛋白

SC-1(小鼠胚胎细胞) 5×106cells/瓶×2 K562(慢性髓原)

丝氨酸/苏氨酸激酶蛋白Pim1抗体规格 CADM3 Others Rat 大鼠 CADM3 / NECL1 / IGSF4B 人细胞裂解液 (阳性对照)

CL-0395NCI-H2227(人小细胞细胞)5×106cells/瓶×2

小鼠肺平滑肌细胞完全培养基 100mL

NIH/3T3小鼠胚胎细胞 NIH/3T3 mouse embryo cells DMEM+10% FBS

IL36B Protein Human 重组人 IL36B / IL1F8 蛋白 (His 标签)

NCI-H2170(人肺鳞癌细胞) 5×106cells/瓶×2 HT-29(细胞)

抗体的生物素化标记实验要点：

1. 丝氨酸/苏氨酸激酶蛋白Pim1抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）丝氨酸/苏氨酸激酶蛋白Pim1抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。