磷酸化前整合位点蛋白1抗体品牌 英文名称  Anti-Phospho-PIM1(Tyr218)

中文名称  磷酸化前整合位点蛋白1抗体品牌 

别    名 Oncogene PIM 1; Oncogene PIM1; PIM 1; Pim 1 kinase; Pim 1 oncogene; PIM; Pim2; PIM3; Proto oncogene serine/threonine protein kinase Pim 1; Proviral integration site 1; Proviral integration site 2.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep

产品类型   一抗 磷酸化抗体  

研究领域     免疫学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 45kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human PIM1 around the phosphorylation site of Tyr218

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化前整合位点蛋白1抗体品牌 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 PIM1 is a member of the serine/threonine kinase PIM oncogene family. PIM1 has been implicated in lymphomagenesis, cell proliferation, apoptosis, differentiation and tumourigenesis. The PIM1 protein kinase is upregulated in prostate cancer.

Function : Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerced by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B,induces 14-3-3-proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis.

Subunit : Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Binds to RP9. Isoform 1, but not isoform 2, binds BMX. Isoform 2 interacts with CDKN1B and FOXO3. Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM1, ubiquitination and proteasomal degradation. Interacts with HSP90, this interaction stabilizes PIM1 protein levels. Interacts (ubiquitinated form) with HSP70 and promotes its proteosomal degradation. Interacts with CDKN1A. Interacts with CDC25C. Interacts (via N-terminal 96 residues) with CDC25A. Interacts with MAP3K5. Interacts with MYC.

Subcellular Location : Isoform 2: Cytoplasm. Nucleus. Isoform 1: Cell membrane.

Post-translational modifications : Autophosphorylated on both serine/threonine and tyrosine residues. Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.

Ubiquitinated, leading to proteasomal degradation.

Similarity : Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.

Contains 1 protein kinase domain.

Database links : UniProtKB/Swiss-Prot: P11309.3

PIM1蛋白是丝氨酸/苏氨酸蛋白激酶2家族成员，他参与细胞信号转导、细胞凋亡、转录调节，在很多中表达，尤其是，表达水平较高。

实验流程：

全、新、优、品、好四大特点：

磷酸化前整合位点蛋白1抗体品牌 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

正在热销的相关产品：

Anti-CD27/TNFRSF7/FITC 荧光素标记CD27抗体IgGMulti-class antibodies规格： 0.2ml

Anti-Hrasls3/HREV107/FITC 荧光素标记HRAS样抑制因子3抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CDAN1 先天性红细胞生成异常性蛋白1抗体 规格 0.2ml

Polycystin 1(Polycystic Kidney Disease 1) 蛋白1抗原 0.5mg

IFIT5 英文名称： 干扰素诱导的三角形四肽重复蛋白5抗体 0.2ml

Rhesus antibody Rh TCF3/E2A/E47 转录因子3抗体(螺旋环螺旋蛋白HE47) 规格 0.2ml

Anti-Hrasls3/HREV107/FITC 荧光素标记HRAS样抑制因子3抗体IgGMulti-class antibodies规格： 0.2ml

Human Immunoglobulin M,IgM ELISA Kit 人免疫球蛋白MMulti-class antibodies规格： 48T

Anti-phospho-RUNX1/AML1(Ser249) 磷酸化急性髓细胞1蛋白抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh GRM2/GLUR2 促代谢型谷氨酸受体2抗体 规格 0.1ml

CEA/CD66(Human carcinoembryonic antign) ELISA Kit 人癌胚抗原 96T

1-Oct 英文名称： 阳离子转运器1抗体 0.1ml

phospho-Band3 (Tyr359) 英文名称： 磷酸化红细胞阴离子交换蛋白1抗体 0.1ml

Anti-phospho-RUNX1/AML1(Ser249) 磷酸化急性髓细胞1蛋白抗体Multi-class antibodies规格： 0.1ml

GAL ELISA Kit 大鼠甘丙肽/甘丙素Multi-class antibodies规格： 48T

Anti-Phospho-Tie2 (Ser1119) 磷酸化生成素受体2抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh glypican 3/GPC3 磷脂酰基醇蛋白聚糖-3抗体 规格 0.1ml

MUC5B(Human mucin-5 subtype B) ELISA kit 人粘蛋白/粘液素5B 96T

phospho-NIFK (Thr230) 英文名称： 磷酸化NIFK抗体 0.1ml

Annexin VI 英文名称： 膜粘连蛋白6抗体 0.1ml

Anti-Phospho-Tie2 (Ser1119) 磷酸化生成素受体2抗体Multi-class antibodies规格： 0.1ml

CL-0087H4(人)5×106cells/瓶×2

IgG2 Others Human 人 IgG2-Fc (257 Ser/Ala) 人细胞裂解液 (阳性对照)

小鼠直肠平滑肌细胞完全培养基 100mL

SV40 MES 13小鼠肾小球系膜细胞 SV40 MES 13 in mouse glomerular mesangial cells DMEM培养基+5%FBS

CF Protein Rat 重组大鼠 CF / Ciliary Neuroophic Factor 蛋白

SC-1(小鼠胚胎细胞) 5×106cells/瓶×2 K562(慢性髓原)

CL-0084FRhK-4(恒河猴胚肾细胞)5×106cells/瓶×2

FAS Others Rat 大鼠 FAS / CD95 / APO-1 / TNFRSF6 人细胞裂解液 (阳性对照)

人平滑肌细胞裂解物HBVSMCL

HO-8910细胞，人细胞 小鼠淋巴结上皮样细胞,SVEC4-10细胞 肾动脉内皮细胞Many types of cells包装：5 × 105次方(1ml)

COLO 320DM(人结直肠腺癌细胞) 5×106cells/瓶×2

HNEpC Pellet 人鼻粘膜上皮细胞团块 > 1 mio.cells 人食道上皮细胞cDNAHEEpiC cDNA

磷酸化前整合位点蛋白1抗体品牌 IGFBP6 Others Mouse 小鼠 IGFBP6 / IBP-6 人细胞裂解液 (阳性对照)

人真皮成纤维细胞-新生儿裂解物HDF-n L

C2C12 小鼠成肌细胞

CL-0110HL-60(人早幼粒急性细胞)5×106cells/瓶×2

人羊膜间充质基质细胞

人胚肾细胞(SV40T基因修饰);293T/17 人主动脉内皮细胞完全培养基 100mL

技术外包服务:

★磷酸化前整合位点蛋白1抗体品牌 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。