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肽基脯氨酞顺反异构酶Pinl抗体说明书 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

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肽基脯氨酞顺反异构酶Pinl抗体说明书 英文名称  Anti-Pin1

中文名称  肽基脯氨酞顺反异构酶Pinl抗体说明书 

别    名 DOD; NIMA interacting 1; Peptidyl prolyl cis trans isomerase NIMA interacting 1; Peptidyl prolyl cis/trans isomerase NIMA interacting; Pin 1; PPIase Pin1; Prolyl isomerase; Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1; Protein NIMA interacting 1; Rotamase Pin1; UBL 5; UBL5; PIN1_HUMAN.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep

产品类型   一抗  

研究领域     免疫学 神经生物学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 18kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Pin1

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

肽基脯氨酞顺反异构酶Pinl抗体说明书 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Pin1 is a Peptidyl-prolyl isomerases (PPIase). Peptidyl-prolyl isomerases (PPIase) facilitate the cis-trans interconversion of the peptidyl-prolyl bond thereby affecting protein folding. Pin1 is a PPIase which specifically recognizes phosphorylated S/T-P bonds.

Pin1 has been implicated in tau pathologies that underlie Alzheimer's Disease. Pin1 binds to tau phosphorylated specifically on the Thr231-Pro site and induces conformational changes in tau. Such conformational changes can directly restore the ability of phosphorylated Tau to bind microtubules and promote microtubule assembly and/or facilitate tau dephosphorylation. Pin1 expression inversely correlates with the predicted neuronal vulnerability in normally aged brain and also with actual neurofibrillary degeneration in AD brain. Pin1 could be pivotal for maintainance of normal neuronal function and preventing age-dependent neurodegeneration.

Function : Essential PPIase that regulates mitosis presumably byinteracting with NIMA and attenuating its mitosis-promotingactivity. Displays a preference for an acidic residue N-terminal tothe isomerized proline bond. Catalyzes pSer/Thr-Pro cis/transisomerizations. Down-regulates kinase activity of BTK. Cantransactivate multiple oncogenes and induce centrosomeamplification, chromosome instability and cell transformation.Required for the efficient dephosphorylation and recycling of RAF1after mitogen activation.

Subunit : Interacts with STIL (By similarity). Interacts withKIF20B. Interacts with NEK6. Interacts (via WW domain) with PRKX.Interacts with BTK. Interacts (via PpiC domain) with DAPK1.Interacts with the phosphorylated form of RAF1. Interacts (via WWdomain) with ATCAY; upon NGF stimulation.

Tissue Specificity : The phosphorylated form at Ser-71 is expressedin normal breast tissue cells but not in breast cancer cells.

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR.Phosphorylation at Ser-71 by DAPK1 results in inhibition of itscatalytic activity, nuclear localization, and its ability to inducecentrosome amplification, chromosome instability and celltransformation.

Similarity : Contains 1 PpiC domain.

Contains 1 WW domain.

Database links : UniProtKB/Swiss-Prot: Q13526.1

肽基脯氨酞顺反异构酶Pinl，是调节细胞有丝分裂相关蛋白酶，对细胞周期运行起着非常重要的调控作用。近年来研究表明，肽基脯氨酞顺反异构酶Pinl在多种中过表达，是多个致癌信号通路的关键效应分子，可加强和催化多种致癌信号，使其无限放大，最终引起细胞转化和增殖失控。因次，肽基脯氨酞顺反异构酶Pinl越来越引起人们关注，被称为发展的催化分子。近来该蛋白用于研究的也日渐增多。

实验流程：

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