产品订购信息：
英文名称  Anti-PDE4A 

中文名称   磷酸二酯酶4A抗体说明书 

别    名  5''-cyclic phosphodiesterase 4A; cAMP specific 3 5 cyclic phosphodiesterase 4A; cAMP specific phosphodiesterase; cAMP-specific 3''; cAMP-specific 3',5'-cyclic phosphodiesterase 4A; DPDE2; dunce like phosphodiesterase E2; PDE 4A; PDE4; PDE46; PDE4A; PDE4A_HUMAN; PDE4A11; Phosphodiesterase 4A; phosphodiesterase 4A cAMP specific; Phosphodiesterase 4A, cAMP-specific (dunce; phosphodiesterase E2 dunce homolog; Phosphodiesterase E2 dunce homolog, Drosophila; phosphodiesterase isozyme 4; phosphodiesterase type 4A11.

浓    度   1mg/1ml

规 格  0.1ml/100μg 0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit 

产品类型   一抗  

研究领域    免疫学 信号转导 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 98kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PDE4A 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸二酯酶4A抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are also important molecular targets for a variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants. Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases. Protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling. In brain members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling. There are 5 isoforms.

Function : Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Subunit : Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

Subcellular Location : Isoform 1: Cytoplasm, perinuclear region.

Isoform 4: Membrane; Peripheral membrane protein. Note=Isoform 4 has propensity for association with membranes.

Isoform 6: Cytoplasm, perinuclear region.

Isoform 7: Cytoplasm. Membrane. Note=Predominantly cytoplasmic.

Tissue Specificity : Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).

Post-translational modifications : Phosphorylation by MAPKAPK2 its activation through PKA phosphorylation (By similarity). Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.

Proteolytically cleaved by caspase-3.

DISEASE : Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).

Similarity : Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

Database links : UniProtKB/Swiss-Prot: P27815.3

Anti-BCRP/ABCG2/BXP-21 /FITC 荧光素标记三磷酸腺苷结合转运蛋白G超家族成员2抗体IgGMulti-class antibodies规格： 0.2ml

Anti-GLP-2/FITC 荧光素标记胰素样肽-2抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CK8 细胞角蛋白8抗体 规格 0.1ml

E7 protein/HPV 16 (Human papillomavirus type 16) 人瘤病毒16型E7蛋白抗原 0.5mg

IFNK 英文名称： 干扰素kappa蛋白抗体 0.2ml

Rhesus antibody Rh TRAILR2/DR5/CD262 细胞调亡素/死亡受体5抗体 规格 0.1ml

Anti-GLP-2/FITC 荧光素标记胰素样肽-2抗体IgGMulti-class antibodies规格： 0.2ml

PDH E1(Human pyruvate dehydrogenase-E1) ELISA Kit 人酸脱氢酶E1Multi-class antibodies规格： 48T

Anti-Phospho-REV-ERB alpha (Ser55/59) 磷酸化细胞核受体Rev-Erbα抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh IGF II/IGF2 样生长因子-II抗体 规格 0.1ml

ANA(Human Anti-nuclear Antibody) ELISA Kit 人抗核抗体 96T

PCNP 英文名称： PEST含核蛋白抗体 0.2ml

CNG3 英文名称： 视锥感光细胞环磷酸鸟苷门控通道蛋白CNG3抗体 0.2ml

Anti-Phospho-REV-ERB alpha (Ser55/59) 磷酸化细胞核受体Rev-Erbα抗体Multi-class antibodies规格： 0.1ml

human phospho-inhibitory subunit of NF-κB Alpha,pIKB Alpha ELISA kit 人磷酸化核因子κB抑制蛋白α(IKKα human ELISA kit)Multi-class antibodies规格： 96T

Anti-Phospho-SHIP2 (Tyr1135) 磷酸化蛋白酪氨酸磷酸酶2抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Goat Anti-rat IgG/PE-Cy7 PE-Cy7标记的羊抗大鼠IgG 规格 0.1ml

MC Ab(Human melanocyte antibody) ELISA Kit 人黑色素细胞抗体 96T

phospho-NFATC4 (Ser168 + Ser170) 英文名称： 磷酸化T细胞激活核转录因子4抗体 0.1ml

BEND3 英文名称： BEND3蛋白抗体 0.2ml

Anti-Phospho-SHIP2 (Tyr1135) 磷酸化蛋白酪氨酸磷酸酶2抗体Multi-class antibodies规格： 0.1ml

CL-0044C2C12(小鼠成肌细胞)5×106cells/瓶×2

GPNMB Others Human 人 GPNMB / Osteoactivin 人细胞裂解液 (阳性对照)

肌细胞分离液MDS

OVAC细胞，人细胞 猴肾C细胞,M145细胞 视网膜微内皮细胞Many types of cells包装：5 × 105次方(1ml)

EB (NBL-4) (牛胚气管细胞) 5×106cells/瓶×2

HREpC Pellet 人类肾小管上皮细胞团块 > 1 mio.cells 人心肌细胞cDNAHCM cDNA

CL-0042BxPC-3(人原位*腺癌细胞)5×106cells/瓶×2

IgG Others Human 人 IgG1 Fc CHO细胞裂解液 (阳性对照)

抗霉菌溶液ABAMS

大额牛肺细胞;BFR-L1 中国仓鼠卵巢细胞，CHO细胞 Lncap(细胞)

EB病毒转化的人B淋巴细胞;KM933

SERPINE1 Others Human 人 PAI-1 / SerpinE1 人细胞裂解液 (阳性对照)

磷酸二酯酶4A抗体说明书 KYNU Others Mouse 小鼠 KYNU / Kynureninase 杆状病毒-昆虫细胞裂解液 (阳性对照)

人脑微内皮细胞总RNAHBMEC NA

大鼠细胞;RH-35

SPC-A-1细胞， 人细胞,HB细胞 tTA基因修饰的小鼠(B类);P19-CAG-tTA-1D3

杂交瘤(B类);IB3C10H12A12G2H8F3

PVRL2 Others Mouse 小鼠 CD112 / PVRL2 人细胞裂解液 (阳性对照)

抗体的生物素化标记实验要点：

1. 磷酸二酯酶4A抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）磷酸二酯酶4A抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。