产品订购信息：
英文名称  Anti-phospho-PRKCZ(Thr500) 

中文名称   磷酸化蛋白激酶C(T500)抗体品牌 

别    名  PRKCZ(phospho T500); rotein Kinase C; AAG6; Aging associated gene 6; MGC129900; MGC129901; MGC41878; MGC57564; PKC B; PKC beta; PKC zeta; PKC2; PKCB; PKCC; PKCD; PKCE; PKCG; PRKACA; PRKC A; PRKC G; PRKCA; PRKCB; PRKCB1; PRKCB2; PRKCD; PRKCE; PRKCG; PRKCZ ; Protein kinase C alpha; Protein kinase C alpha type; Protein Kinase C; Protein kinase C beta 1; Protein kinase C beta 1 polypeptide; Protein kinase C beta; Protein kinase C beta type; Protein kinase C delta; Protein kinase C epsilon; Protein kinase C gamma; Protein kinase C gamma type; Protein kinase C zeta; SCA14; Spinocerebellar ataxia 14; KPCB_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Cow, Horse, Rabbit 

产品类型   一抗 磷酸化抗体  

研究领域     细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 77kDa

性    状   Lyophilized or Liquid

免 疫 原 LH conjugated Synthesised phosphopeptide derived from human PRKCZ around the phosphorylation site of Thr500 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化蛋白激酶C(T500)抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Function : Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity).

Subunit : Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1. Interacts with PHLPP1 and PHLPP2; both proteins mediate its dephosphorylation. Interacts with KDM1A/LSD1, PKN1 and ANDR.

Subcellular Location : Cytoplasm. Nucleus. Membrane; Peripheral membrane protein.

Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 C2 domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

Database links : UniProtKB/Swiss-Prot: P05771.4

Anti- Beta-catenin/ Beta-cats /FITC 荧光素标记 β-连环蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Anti-HCMV UL23/HHV5 UL23/FITC 荧光素标记人巨细胞病毒UL23抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Centaurin γ1 中心体肌动蛋白γ1抗体(增强型PI3K蛋白) 规格 0.2ml

Tie2(Porcine tyrosine kinase with immunoglobulin-like and EGF-like domains 2) 上皮生长因子样域酪酸激酶2抗原 0.5mg

IGF1R 英文名称： 样生长因子1受体抗体 0.1ml

Rhesus antibody Rh TIS11D/ERF2 表皮生长因子反应蛋白2抗体 规格 0.2ml

Anti-HCMV UL23/HHV5 UL23/FITC 荧光素标记人巨细胞病毒UL23抗体IgGMulti-class antibodies规格： 0.2ml

HB- Beta(Human Beta-subunits of hemoglobin) ELISA Kit 人血红蛋白β亚基Multi-class antibodies规格： 48T

Anti-Phospho-RasGRF1 (Ser916) 磷酸化Ras特异性鸟嘌呤核苷酸释放因子1Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh HA tag HA tag标签抗体 规格 0.1ml

MUC5AC(Human Mucin-5 subtype AC) ELISA Kit 人粘蛋白/粘液素5AC 96T

PRR15 英文名称： 富含脯酸蛋白15抗体 0.2ml

BTNL8 英文名称： 嗜乳脂蛋白样8抗体 0.2ml

Anti-Phospho-RasGRF1 (Ser916) 磷酸化Ras特异性鸟嘌呤核苷酸释放因子1Multi-class antibodies规格： 0.1ml

IL-4 ELISA KIT 大鼠白介素4Multi-class antibodies规格： 48T

Anti-p-BAD/FITC 荧光素标记抗磷酸化相关死亡促进因子p-BAD抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh ETK/BMX 非受体性蛋白酪氨酸激酶ETK抗体 规格 0.1ml

IAP(Human inhibitor of apoptosis) ELISA Kit 人细胞凋亡抑制因子 96T

Phospho-MYPT1 (Ser507) 英文名称： 磷酸化肌球蛋酸酶抗体 0.1ml

Angiotensin II 英文名称： 紧张素Ⅱ抗体 0.1ml

Anti-p-BAD/FITC 荧光素标记抗磷酸化相关死亡促进因子p-BAD抗体IgGMulti-class antibodies规格： 0.2ml

CL-00074T1(小鼠癌细胞)5×106cells/瓶×2

EFNA5 Others Rat 大鼠 Ephrin-A5 / EFNA5 人细胞裂解液 (阳性对照)

小鼠细胞完全培养基 100mL

TM3小鼠间质细胞 TM3 mouse Leydig cells D-MEM/F-12培养基(GIBCO)+5%马血清+2.5%FBS

IL20 Protein Human 重组人 IL20 / Ierleukin-20 蛋白

SNU-5(人细胞) 5×106cells/瓶×2 293(转染腺病毒E1A基因的人肾上皮细胞)

CL-000422RV1(人细胞)5×106cells/瓶×2

FKBP14 Others Human 人 FKBP14 人细胞裂解液 (阳性对照)

尿道上皮细胞生长添加物UCGS

HEC-1-A细胞，人子宫内膜腺癌细胞 鼠杂交细胞,K6H6/B5细胞 新生儿细胞Many types of cells包装：5 × 105次方(1ml)

FC33(人胚胎肾细胞(Asp-2基因修饰)) 5×106cells/瓶×2

HWP subcutaneous Pellet 人类皮下白前脂肪细胞团块 > 1 mio.cells 人少突胶质前体细胞(悬浮生长)总RNAHOPC-os NA

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CM-H111人成骨细胞完全培养基100mL

人肾上腺皮质细胞HACC

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人癌细胞;MCF7

TNFRSF4 Others Human 人 TNFRSF4 / CD134 人细胞裂解液 (阳性对照)

抗体的生物素化标记实验要点：

1. 磷酸化蛋白激酶C(T500)抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及*基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）磷酸化蛋白激酶C(T500)抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。